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Development of Marine Toxicity Standard Method for Marine Luminescent Bacteria: Introduction of N-Tox test  

Lee, Kyu-Tae (NeoEnBiz Co.)
Park, Gyung-Soo (Department of Marine Biotechnology, Anyang University)
Kim, Pyoung-Joong (Environment Research Division, West Sea Fisheries Research Institute, NFRDI)
Publication Information
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY / v.13, no.2, 2008 , pp. 156-163 More about this Journal
Abstract
Luminescent bacterial toxicity test was first introduced in the early 1980s, registered as international standard method in 1998 and now widely used as a common toxicity test method. This toxicity test uses luminescent bacterium, Vibrio fischeri, originated from marine environment as a test organism. The degree of toxicity can be evaluated from the comparison of luminescent emission intensity between control and treatment groups to toxicants and materials from various environmental matrix for 30 min. This test can be carried out by using commercial products and its results are sensitive and precise. This research is on the feasibility of adopting luminescent bacterial test as a domestic standard test protocol. Using commercial products, a series of experiments were conducted to identify the precision and accuracy of injection volume and light emission, and to evaluate concentration-response relationship between chemical concentrations and light emissions. Also, the feasibility of the application to environmental media and quality assurance/quality control were checked. The results of serial toxicity tests revealed that the preliminary luminescent bacterial toxicity test was robust and suitable as a standard method.
Keywords
Luminescent Bacteria; Vibrio fischeri; Toxicity Test; Bioassay; Standard Method;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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