• Journal of Practical Agriculture & Fisheries Research
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• v.24 no.1
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• pp.14-22
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• 2022

The purpose of this study is to provide evidence for discovering functional materials through the anti-inflammatory efficacy screening of randomly selected medicinal herbs. We prepared 70% ethanol extracts from 10 herbs and evaluated for the inhibitory effect of NO production on LPS-stimulated mouse macrophage cell line Raw 264.7. As a result, it was confirmed that the Chrysanthemi Zawadskii Herba (CZ) extract had the highest effect of inhibiting NO production induced by LPS. We therefore measured and compared NO inhibitory effects at different concentrations (10, 50, 250 ㎍/mL) of 70% ethanol and water extract of CZ. It was observed that both ethanol and water treatment groups inhibited NO production in a concentration-dependent manner in both ethanol and water treatment groups. In particular, it was confirmed that the CZ 70% ethanol extract (99.97%) had a higher NO inhibitory effect than the water extract (93.32%) in the high concentration (250 ㎍/mL) treatment group. There was no effect of CZ extract on cell viability at all concentrations used in the experiment. Moreover, it was shown that CZ ethanol extract remarkably inhibited the expression of VCAM-1 induced by TNF-𝛼, and it was slightly decreased even by treatment with water extract. This study suggests that Chrysanthemi Zawadskii Herba has potential as a functional substance that regulates vascular inflammation.

• Journal of the Korean Society of Marine Environment & Safety
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• v.30 no.4
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• pp.308-316
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• 2024

Maintaining the appropriate number of national seafarers is essential for securing the competitiveness of maritime industry and security in Korea. However, the number of national seafarers in Korea has been decreased continuously recently. The government recognizes the repercussion of this issue and has been attempting to solve it. One of the most significant reasons contributing to the rapid decrease in national seafarers is presumed to be the high initial turnover rate due to the decreased preference for maritime careers among people in the 20s and 30s. Therefore, plans have been devised to prepare various policies that allow young people to select long-term boarding. This study analyzed the determinants of long-term boarding decisions among students enrolled in the Maritime College of 'M' University and verifies the relationship with the desired boarding period. Additionally, the crew composition (CC), long-term planning (LP), work environment (WE), and family environment (FE) were derived as the determinants of long-term boarding recognized by prospective seafarers. Among them, LP and WE significantly affect the desired boarding period, thus suggesting that the stronger the perception toward long-term planning and the more insensitive one is to the work environment, the longer is the desired boarding period. In terms of group differences in the perception of long-term boarding, analysis results show that the several determinants of long-term boarding are recognized differently depending on gender. This study may facilitate the preparation of factors and group-specific policy measures to promote long-term boarding among prospective seafarers.

• Journal of the Institute of Convergence Signal Processing
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• v.3 no.2
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• pp.83-88
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• 2002

In this paper, a POS interfacing and temperature compensable LPG dispensing control system(LDCS) has been developed. A LDCS includes a 16-bit 80C196 microprocessor, RAM, ROM, video driver, and programmable peripheral devices. Based on gas flow encoding pulse, temperature-voltage conversion values and apparatus calibration values, the LDCS controls the LPG dispensing quantity with switching on or off the solenoid valves. The temperature compensation is performed with a 10-bit A/D conversion and its range is from +7$0^{\circ}C$ to -3$0^{\circ}C$ with a 0.5$^{\circ}C$ resolution.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.11
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• pp.1506-1511
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• 2018

This study presents a human sensibility evaluation method using neural network and multiple-template method on electroencephalogram(EEG). We used a multi-layer perceptron type neural network as the sensibility classifier using EEG signal. For our research objective, 10-channel EEG signals are collected from the healthy subjects. After the necessary preprocessing is performed on the acquired signals, the various EEG parameters are estimated and their discriminating performance is evaluated in terms of pattern classification capability. In our study, Linear Prediction(LP) coefficients are utilized as the feature parameters extracting the characteristics of EEG signal, and a multi-layer neural network is used for indicating the degree of human sensibility. Also, the estimation for human comfortableness is performed by varying temperature and humidity environment factors and our results showed that the proposed scheme achieved good performances for evaluation of human sensibility.

• Journal of Digital Convergence
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• v.11 no.8
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• pp.273-279
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• 2013

The purpose of the study was to present basic data to evaluation of the spatial resolution for exposure class(EC) in computed radiography(CR) by using the modulation transfer function(MTF). In this study, MTF was measured the edge method by using image plate(IP) of $100{\mu}mm$ pixels. A standard beam quality RQA5 based on an international electro-technical commission(IEC) standard was used to perform the X-ray imaging studies. Digital imaging began to set the sensitivity to EC 50, 100, 200, 300, 400, 600, 800, 1200 in X-ray irradiated to IP. The MTF 50% and 10% in the final images was analysis by using an authorized image analysis program the Origin 8.0 and the image J. As a results, the EC 200 was the best spatial resolution at MTF 50% ($1.979{\pm}0.114lp/mm$) and MTF 10% ($3.932{\pm}0.041$). Therefore, the EC 200 could be useful for the diagnosis of diseases that require high spatial resolution such as fractures.

• Nutrition Research and Practice
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• v.18 no.1
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• pp.46-61
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• 2024

BACKGROUND/OBJECTIVES: An increasing life expectancy in society has burdened healthcare systems substantially because of the rising prevalence of age-related metabolic diseases. This study compared the effects of animal protein hydrolysate (APH) and casein on metabolic diseases using aged mice. MATERIALS/METHODS: Eight-week-old and 50-week-old C57BL/6J mice were used as the non-aged (YC group) and aged controls (NC group), respectively. The aged mice were divided randomly into 3 groups (NC, low-APH [LP], and high-APH [HP] and fed each experimental diet for 12 weeks. In the LP and HP groups, casein in the AIN-93G diet was substituted with 16 kcal% and 24 kcal% APH, respectively. The mice were sacrificed when they were 63-week-old, and plasma and hepatic lipid, white adipose tissue weight, hepatic glucose, lipid, and antioxidant enzyme activities, immunohistochemistry staining, and mRNA expression related to the glucose metabolism on liver and muscle were analyzed. RESULTS: Supplementation of APH in aging mice resulted in a significant decrease in visceral fat (epididymal, perirenal, retroperitoneal, and mesenteric fat) compared to the negative control (NC) group. The intraperitoneal glucose tolerance test and area under the curve analysis revealed insulin resistance in the NC group, which was alleviated by APH supplementation. APH supplementation reduced hepatic gluconeogenesis and increased glucose utilization in the liver and muscle. Furthermore, APH supplementation improved hepatic steatosis by reducing the hepatic fatty acid and phosphatidate phosphatase activity while increasing the hepatic carnitine palmitoyltransferase activity. Furthermore, in the APH supplementation groups, the red blood cell (RBC) thiobarbituric acid reactive substances and hepatic H₂O₂ levels decreased, and the RBC glutathione, hepatic catalase, and glutathione peroxidase activities increased. CONCLUSIONS: APH supplementation reduced visceral fat accumulation and alleviated obesity-related metabolic diseases, including insulin resistance and hepatic steatosis, in aged mice. Therefore, high-quality animal protein APH that reduces the molecular weight and enhances the protein digestibility-corrected amino acid score has potential as a dietary supplement for healthy aging.

• Journal of Digital Convergence
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• v.16 no.7
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• pp.309-316
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• 2018

The purpose of this study was to investigate the effects of Violae Herba Water Extract (VH) on the proinflammatory factors of lipopolysaccharide (LPS)-induced on the production of inflammatory mediators in RAW 264.7 mouse macrophages cells. We examined effect of Violae Herba Water Extract on the cell viability of RAW 264.7 mouse macrophages cells. Futhermore, After 24 hours treatment we investigated anti-inflammatory effect of Violae Herba Water Extract by the production of Bio-Plex cytokine assay, concentrations of various cytokines such NO, $interleukin(IL)-1{\beta}$, tumor necrosis factor ${\alpha}(TNF-{\alpha})$ and IL-6. The water extract of Violae Herba significantly inhibited the production of NO, $IL-1{\beta}$, $TNF-{\alpha}$ and IL-6 at the concentration of 25, 50, 100 and $200{\mu}g/mL$ in the LPS-induced RAW 264.7 mouse macrophages cells with no changes in the cell viability of them. These results suggest that water extract of Violae Herba has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as $IL-1{\beta}$, $TNF-{\alpha}$ and IL-6 in the LPS-induced RAW 264.7 mouse macrophages cells. Further research is needed to develop therapeutic agents for inflammatory diseases using Violae Herba.

• Journal of Digital Convergence
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• v.16 no.11
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• pp.613-620
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• 2018

This study was conducted to investigate of Mori Folium Water Extract (MF) on anti-inflammation activity. MF Water extracts after 24 houres cultivation were examined to ascertain the cell viability of mouse macrophage RAW 264.7 cells. The influence of the Water extracts in RAW 264.7 macrophage cells treated with LPS was investigated. nitric oxide (NO) production, nterleukin$(IL)-1{\alpha}$ IL-6 and IL-10 increased generation of cytokines. mouse macrophage RAW 264.7 cells cell viability changes were no decreas after MTT assay of MF Water extract. The MF water extracts inhibited NO generation caused by LPS in the macrophages over $25{\mu}g/mL$. The MF water extracts increased in the control group the $IL-1{\alpha}$ and IL-6 activation generated by LPS in the macrophages over $50{\mu}g/mL$. Accordingly, it was found that different MF water extract concentrations significantly influenced certain anti-inflammation activities in RAW 264.7 macrophage cells. The results of this study are expected to be highly applicable to health - friendly functional materials. Further studies are needed to confirm the signaling pathways associated with anti-inflammation of macrophages through continuous studies.

• Journal of the Korean Society of Food Culture
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• v.30 no.2
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• pp.197-205
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• 2015

This study was conducted to investigate the bioconversion of ginsenosides as well as anti-inflammatory activities of fresh ginseng Kkakdugi during fermentation. Fresh ginseng Kkakdugi reached proper ripeness, pH 4.30, and acidity 1.69% at $15^{\circ}C$ after 10 days. Lactic acid bacteria grew until reaching $1.10{\times}10^9CFU/mL$ after 20 days of fermentation, and ${\beta}$-glucosidase activity increased from 1.154 to 1.885 units/g. The bioconversion of ginsenosides was confirmed based on increased content of Rg3, an aglycone, from 0.13 to 0.17 mg/g during fermentation through HPLC. Fresh ginseng Kkakdugi did not display cytotoxicity up to the concentrations of $80{\mu}g/mL$, regardless of ripening period. Nitrite production and expression of inflammation-related proteins, iNOS and COX-2, decreased in a dose-dependent manner regardless of ripening period. From these results, fresh ginseng Kkakdugi showed the bioconversion of ginsenosides to aglycone during the lactic acid fermentation as well as an anti-inflammatory effect through the reduction of NO production and iNOS and COX-2 expression.

• Journal of the Korea Convergence Society
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• v.8 no.1
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• pp.239-243
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• 2017

This study aims to demonstrate the inhibitory effect of proinflammatory cytokines by using Frankinsense. The present data was designed to determine the production of the frankincence on pro-inflammatory factors such as $TNF-{\alpha}$ and $IL-1{\beta}$ in lipopolysaccharide(LPS) stimulated RAW264.7 macrophages cell. The cell toxicity was identified by CellTiter 96 AQueous One solution cell proliferation assay. To evaluate of anti-inflammatory effect of frankincence, pro-inflammatory cytokines were measured by ELISA kit. As a result, the frankincence reduced NO and $TNF-{\alpha}$ production without cytotoxicity. As a result, Francincense was not cytotoxic at 10 ug / ml-1000 ug / ml and significantly inhibited the proinflammatory cytokines $TNF-{\alpha}$ and $IL-1{\beta}$. The secretion inhibition effect of proinflammatory cytokine is believed to be applicable to various physiological activity data and functional materials to demonstrate the anti - inflammatory properties of frankincense.