Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Regulatory Effects of Chrysanthemi Zawadskii Herba on NO Production and Vascular Adhesion Molecule Expression

구절초(Chrysanthemi Zawadskii Herba)의 항염증 인자 생성 및 혈관부착인자 발현 억제 효과

  • Sohn, E.S. (Center for Global R&D Data Analysis, Korea Institute of Science and Technology Information) ;
  • Kim, S.H. (Department of Bio-Health Convergence, Kangwon National University) ;
  • Ha, C.W. (Department of Bio-Health Convergence, Kangwon National University) ;
  • Jang, S. (Department of Bio-Health Convergence, Kangwon National University) ;
  • Sohn, E.H. (Department of Bio-Health Convergence, Kangwon National University) ;
  • Chae, C.J. (Department of Liberal Arts, Korea National University of Agriculture and Fisheries) ;
  • Koo, H.J. (Department of Crops and Forestry, Korea National College of Agriculture & Fisheries)
  • 손은수 (한국과학기술정보연구원) ;
  • 김성혁 (강원대학교 바이오헬스융합학과) ;
  • 하창우 (강원대학교 바이오헬스융합학과) ;
  • 장소희 (강원대학교 바이오헬스융합학과) ;
  • 손은화 (강원대학교 바이오헬스융합학과) ;
  • 채철주 (국립한국농수산대학교 교양학부) ;
  • 구현정 (국립한국농수산대학교 작물산림학부)
  • Received : 2022.03.19
  • Accepted : 2022.04.05
  • Published : 2022.04.18
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Abstract

The purpose of this study is to provide evidence for discovering functional materials through the anti-inflammatory efficacy screening of randomly selected medicinal herbs. We prepared 70% ethanol extracts from 10 herbs and evaluated for the inhibitory effect of NO production on LPS-stimulated mouse macrophage cell line Raw 264.7. As a result, it was confirmed that the Chrysanthemi Zawadskii Herba (CZ) extract had the highest effect of inhibiting NO production induced by LPS. We therefore measured and compared NO inhibitory effects at different concentrations (10, 50, 250 ㎍/mL) of 70% ethanol and water extract of CZ. It was observed that both ethanol and water treatment groups inhibited NO production in a concentration-dependent manner in both ethanol and water treatment groups. In particular, it was confirmed that the CZ 70% ethanol extract (99.97%) had a higher NO inhibitory effect than the water extract (93.32%) in the high concentration (250 ㎍/mL) treatment group. There was no effect of CZ extract on cell viability at all concentrations used in the experiment. Moreover, it was shown that CZ ethanol extract remarkably inhibited the expression of VCAM-1 induced by TNF-𝛼, and it was slightly decreased even by treatment with water extract. This study suggests that Chrysanthemi Zawadskii Herba has potential as a functional substance that regulates vascular inflammation.

본 연구에서는 시중에 유통되는 생약 10종 (금전초, 목천료, 냉초, 현초, 계심, 진피, 율초, 구절초, 총백, 고량강)을 무작위로 선정하여 생약의 생리활성 효과를 스크리닝하였다. 각 생약을 70% 에탄올로 추출한 다음 추출물의 마우스 대식세포에 대한 NO 생성 억제 효능을 확인한 결과, 구절초 추출물이 LPS에 의해 유도된 NO 생성을 억제하는 효과가 가장 높은 것으로 확인되었다. 따라서, 구절초 70% ethanol 및 물 추출물에 대한 농도별 NO 생성 억제 효능을 측정하였다. 구절초 추출물은 두 용매 추출물 모두에서 대식세포의 NO 조절 효과가 우수한 것으로 나타났으며, 70% ethanol 추출물의 고농도 (250 ㎍/mL) 처리군에서는 LPS에 의해 유도된 NO를 99% 이상 억제하는 것으로 확인되었다. 또한, 구절초 70% ethanol 및 물 추출물이 인체 대동맥 평활근 세포주 HASMCs에서 TNF-α에 의한 부착인자의 발현 억제 효능을 확인하였다. 그 결과, 구절초의 70% ethanol 추출물 및 물 추출물이 TNF-α로 자극된 인체 대동맥평활근 세포주 HASMC에서 세포 부착 인자의 발현을 억제하였으며, 이 결과는 구절초가 혈관 염증을 조절할 수 있는 가능성을 제시한다. 본 연구 결과는 구절초의 항염증 및 혈관 염증 조절 기능 소재로서 개발을 위한 기초 자료로 활용될 수 있을 것으로 사료된다.

Keywords

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