Browse > Article
http://dx.doi.org/10.14400/JDC.2018.16.7.309

Effect of Violae Herba Water Extract on the Proinflammatory Factors of LPS-Induced Macrophages  

Han, Hyo-Sang (Department of Health Administration, Joongbu University)
Publication Information
Journal of Digital Convergence / v.16, no.7, 2018 , pp. 309-316 More about this Journal
Abstract
The purpose of this study was to investigate the effects of Violae Herba Water Extract (VH) on the proinflammatory factors of lipopolysaccharide (LPS)-induced on the production of inflammatory mediators in RAW 264.7 mouse macrophages cells. We examined effect of Violae Herba Water Extract on the cell viability of RAW 264.7 mouse macrophages cells. Futhermore, After 24 hours treatment we investigated anti-inflammatory effect of Violae Herba Water Extract by the production of Bio-Plex cytokine assay, concentrations of various cytokines such NO, $interleukin(IL)-1{\beta}$, tumor necrosis factor ${\alpha}(TNF-{\alpha})$ and IL-6. The water extract of Violae Herba significantly inhibited the production of NO, $IL-1{\beta}$, $TNF-{\alpha}$ and IL-6 at the concentration of 25, 50, 100 and $200{\mu}g/mL$ in the LPS-induced RAW 264.7 mouse macrophages cells with no changes in the cell viability of them. These results suggest that water extract of Violae Herba has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as $IL-1{\beta}$, $TNF-{\alpha}$ and IL-6 in the LPS-induced RAW 264.7 mouse macrophages cells. Further research is needed to develop therapeutic agents for inflammatory diseases using Violae Herba.
Keywords
Violae Herba; Cytokine; Nitric oxide; Anti-inflammatory effect; Macrophage;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 J. B. Calixto, M. M. Campos, M. F. Otuki & A. R. Santos. (2004). Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion melecules. Planta Med, 70(2), 93-103.   DOI
2 W. P. Arend & J. M. Daye. (2005). Cytokines and cytokine inhibitors or antagonists in rheumatoid arthritis, Arthritis and Rheumatism, 33(3), 305-15.   DOI
3 Y. B Lee et al. (2013). The Effect of Mulberry Leaf Extract on Blood biochemical parameters in White Rats Exposed to 2,3,7,8-tetrachlorodibenzo-pdioxin(TCDD). Journal of Digital Convergence, 11(1), 299-308.   DOI
4 J. Kim. (2014). Antibacter ial and anti-inflammatory effects of Platycodon grandiflorum extracts. Journal of Digital Convergence, 12(3), 359-366.   DOI
5 A. Y Jang, Y. C. Sueng & J. G. Ji. (2016). The comparative study on physiological activity of White ginseng, Red ginseng and Black ginseng extract. Journal of Digital Convergence, 14(5), 459-471.   DOI
6 M. S. Kim, S, H. Park & H. R. Park. (2016). Convergence Studies Vascular Relaxation and Safty Evaluation in Viscum Coloratumma, Chrysantheum Morifolium, Citri Percarpium, and Ophiopoginis Radix Mixture. Journal of Digital Convergence, 14(9), 479-484.   DOI
7 S. H. Park, B. J. Park & H. R. Park. (2016). Studies on Nutritional Analysis and Antioxidant activity of Oriental Medicines with Bloodstream Improvement. Journal of Digital Convergence, 14(10), 563-570.   DOI
8 T. Mosmann. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65(1-2), 55-63.   DOI
9 J. A. Politch, L. Tucker, F. P. Bowman & D. J. Anderson. (2007). Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men. Hum Reprod, 22(11), 2928-2935.   DOI
10 B, B Lee et al. (2008). Antioxidant Activity and Inhibition Activity against ${\alpha}$-Amylase and ${\alpha}$-Glucosidase of Viola mandshurica Extracts. J Korean Soc Food Sci Nutr, 37(4), 405-409.   DOI
11 J. N. Sharma, A. Al-Omran & S. S. Parvathy. (2007). Role of nitric oxide in inflammatory diseases. Inflammopharmacol, 15(6), 252-259.   DOI
12 K. S. Ko. (2012). A study on Antioxidant Effect of Methanol Extract from Viola Mandshurica. Journal of the korean socity of Cosmetology, 18(5), 1082-1086.
13 M. R. Lee, J. H. Hwang, J. H. Park, H. J. Kim, E. J. Park & H. R. Park. (2010). Antioxidant Activity and Protective Effects of 9-hydroxy-${\alpha}$-tocopherone from Viola mandshurica Extracts. Kor J Pharmacogn, 41(3), 166-173.
14 J. M. Mccord, K. Wong, S. H. Stokes, W. F. Petrone & D. English. (1980). Superoxide and inflammation: a mechanism for the anti-inflammatory activity of superoxide dismutase. Acta Physiol Scand Supp, 492, 25-30.
15 R. Korhonen, A. Lahti, H. Kankaanranta & E. Moilanen. (2005). Nitric oxide production and signaling in inflammation. Curr Drug Targets Inflamm Allergy, 4(4), 471-479.   DOI
16 C. A. Dinarello. (2000). Proinflammatory cytokines. Chest, 118(2), 503-508.   DOI
17 W. P. Arend & J. M. Dayer. (1995). Inhibition of the production and effects of interleukin-1 and tumor necrosis factor ${\alpha}$ in rheumatoid arthritis. Arthritis Rheum, 38(2), 151-160.   DOI
18 F. R. Balkwill, M. S. Maylor & S. Malik. (1990). Tumor necrosis factor as an anti-cancer agent. European Journal of Cancer and Clinical Oncology, 26(5), 641-644.   DOI
19 G. E. Nedwin, L. P. Svedersky, T. S. Bringman, M. A. Jr. Palladino & D. V. Goeddel. (1985). Effect of interleukin 2, interferon-gamma and mitogens on the production of tumor necrosis factors alpha and beta. J Immunol, 135(4), 2492-2497.
20 I. R. Kim et al. Boncho-Hak. Seoul : Young-Lim Press.
21 Korea Food and Drug Administration. The Korean Herbal Pharmacopoeia. Seoul : Korea Food and Drug Administration.
22 B. W. Lee & D. H. Kim. (2003). Inhibitory effect of Violae herba extract on inflammatory cytokine production induced by IL-$1{\beta}$ and TNF-${\alpha}$ in culured human synovial cells. Kor J Herbology, 18(3), 89-96.
23 M. Liu, J. Li, F. Kong, J. Lin & Y. Gao. (1998). Induction of immunomodulating cytokines by a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes. Immunopharmacology, 40(3), 187-198.   DOI
24 C. A. Dinarello, S. Endres, S. N. Meydani, M. Meydani & M. K. Hellerstein. (1990). Interleukin-1, anorexia, and dietary fatty acids. Ann N Y Acad Sci, 587, 332-338.   DOI
25 J. S. Ko. (2012). A Study on Antioxidant Effect of Methanol Extract from Viola Mandshurica. Journal of The Korea Society of Cosmetology, 18(5), 1082-1086.
26 D. S. DS et al. (2011). Neuronal Cytoprotective Effect of Violae Herba Origin Plants. Kor J Oral Maxillofac Patho, 35(4), 209-214.
27 M. Y. Lee et al. (2010). Anti-inflammatory and anti-asthmatic effects of Viola mandshurica W. Becker(VM) ethanolic (EtOH) extract on airway inflammation in a mouse model of allergic asthma. Journal of Ethnopharmacology, 127, 159-164.   DOI
28 M. J. Rang. (2013). Anti-inflammatory and Anti-allergic Effects of Herbal Extracts on Atopic Dermatitis ( Part II ). J of Korean Oil Chemists' Soc, 30(1), 173-182.   DOI
29 Y. Y. Sung, D. S. Kim & H. K. Kim. (2014). Viola mandshurica ethanolic extract prevents high-fat-diet-induced obesity in mice by activating AMP-activated protein kinase. Environ Toxicol Pharmacol, 38(1), 41-50.   DOI
30 S. S. No. (2006). Wonsaek Pibugaw-Hak. Seoul : IBC Gihoek Press.
31 J. B. Jeong, S. C. Hong, H. J. Jeong & J. S. Koo. (2012). Anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino on LPS-stimulated RAW 264.7 and THP-1 cells. Korean J Plant Res, 25(3), 299-307.   DOI
32 H. Matsuda, T. Morikawa, S. Ando, I. Toguchida & M. Yoshikawa. (2003). Structural requirements of flavonoids for nitric oxide production inhibitory activity and mechanism of action. Bioorg Med Chem, 11(9), 1995-2000.   DOI