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http://dx.doi.org/10.23097/JPAF.2022.24(1):14

Regulatory Effects of Chrysanthemi Zawadskii Herba on NO Production and Vascular Adhesion Molecule Expression  

Sohn, E.S. (Center for Global R&D Data Analysis, Korea Institute of Science and Technology Information)
Kim, S.H. (Department of Bio-Health Convergence, Kangwon National University)
Ha, C.W. (Department of Bio-Health Convergence, Kangwon National University)
Jang, S. (Department of Bio-Health Convergence, Kangwon National University)
Sohn, E.H. (Department of Bio-Health Convergence, Kangwon National University)
Chae, C.J. (Department of Liberal Arts, Korea National University of Agriculture and Fisheries)
Koo, H.J. (Department of Crops and Forestry, Korea National College of Agriculture & Fisheries)
Publication Information
Journal of Practical Agriculture & Fisheries Research / v.24, no.1, 2022 , pp. 14-22 More about this Journal
Abstract
The purpose of this study is to provide evidence for discovering functional materials through the anti-inflammatory efficacy screening of randomly selected medicinal herbs. We prepared 70% ethanol extracts from 10 herbs and evaluated for the inhibitory effect of NO production on LPS-stimulated mouse macrophage cell line Raw 264.7. As a result, it was confirmed that the Chrysanthemi Zawadskii Herba (CZ) extract had the highest effect of inhibiting NO production induced by LPS. We therefore measured and compared NO inhibitory effects at different concentrations (10, 50, 250 ㎍/mL) of 70% ethanol and water extract of CZ. It was observed that both ethanol and water treatment groups inhibited NO production in a concentration-dependent manner in both ethanol and water treatment groups. In particular, it was confirmed that the CZ 70% ethanol extract (99.97%) had a higher NO inhibitory effect than the water extract (93.32%) in the high concentration (250 ㎍/mL) treatment group. There was no effect of CZ extract on cell viability at all concentrations used in the experiment. Moreover, it was shown that CZ ethanol extract remarkably inhibited the expression of VCAM-1 induced by TNF-𝛼, and it was slightly decreased even by treatment with water extract. This study suggests that Chrysanthemi Zawadskii Herba has potential as a functional substance that regulates vascular inflammation.
Keywords
Medicinal herb; Chrysanthemi Zawadskii Herba; Anti-inflammation; Vascular adhesion molecule;
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