• Food Science of Animal Resources
• /
• v.38 no.5
• /
• pp.878-888
• /
• 2018

In the current study, the probiotic potential of approximately 250 strains of lactic acid bacteria (LAB) isolated from piglet fecal samples were investigated; among them Lactobacillus plantarum strain JDFM LP11, which possesses significant probiotic potential, with enhanced acid/bile tolerance, attachment to porcine intestinal epithelial cells (IPEC-J2), and antimicrobial activity. The genetic characteristics of strain JDFM LP11 were explored by performing whole genome sequencing (WGS) using a PacBio system. The circular draft genome have a total length of 3,206,883 bp and a total of 3,021 coding sequences were identified. Phylogenetically, three genes, possibly related to survival and metabolic activity in the porcine host, were identified. These genes encode p60, lichenan permease IIC component, and protein TsgA, which are a putative endopeptidase, a component of the phosphotransferase system (PTS), and a major facilitator in the gut environment, respectively. Our findings suggest that understanding the functional and genetic characteristics of L. plantarum strain JDFM LP11, with its candidate genes for gut health, could provide new opportunities and insights into applications in the animal food and feed additive industries.

• Journal of Digital Convergence
• /
• v.13 no.4
• /
• pp.369-375
• /
• 2015

In this study, the formation potential and the stability of nano-emulsions were evaluated according to the structure of various cosmetic oils in Tween 80/Span 80 system using PIC method at 80 oC LP 70, Isopar H and Pripure 3759 of hydrocarbons were both form a stable nano-emulsion, particle size distribution of about 40 nm. A linear structure of silicone oil formed an unstable emulsion, but cyclic or short chain oil formed was a stable nano-emulsion. In ester oils, the particle size of emulsions increases with increasing molecular weight of oils and a stable nano-emulsion could not be obtained in the molecular weight of about 450. The particle size of the nano-emulsion against required HLB value for calculating in consideration of the lipophilic and hydrophilic oil was smaller in the HLB of 8-10.

• Journal of Korean Society of Disaster and Security
• /
• v.10 no.2
• /
• pp.29-34
• /
• 2017

LPG is a substance that requires a lot of attention because it can cause fatal damage to people and environment when an accident occurs. LPG is frequently accidents in transportation facilities as well as fixed facilities, among which LPG tank lorries are the most frequent accidents. When the LPG tank is evacuated, the LP gas leaks into two phases, leaks mostly to the gas and leaks to some liquid. At this time, the leaked gas will also sink downward because it is heavier than air, and if it continues to leak, it may form an explosion and explode by the ignition source. The purpose of this study is to present the evacuation distance by analyzing the effect distance of the LPG liquefied petroleum gas in the event of explosion. As a result of calculation of the scattering radius of the fragment, the cylinder fragment was scattered up to 561 m. Therefore, it is appropriate to set the distance to be escaped when the LPG tanker leaks to 561m or more.

• Journal of Life Science
• /
• v.25 no.9
• /
• pp.961-969
• /
• 2015

Epigallocatechin gallate (EGCG), the main catechin in green tea, has been shown to have some beneficial effects against various human diseases, including diabetes, neurodegenerative disorders, cancer, cardiovascular disease and obesity. To investigate the mechanism of the suppressive effects of EGCG on inflammatory response in macrophages, alterations on the levels of nitric oxide (NO) regulatory factors and inflammatory cytokines were measured in lipopolysaccharide (LPS)-activated RAW264.7 cells. No significant toxicity was detected in RAW264.7 cells treated with 100–400 μM EGCG. Moreover, the optimal concentration of LPS was determined to be 1 μg/ml based on the results of cell viability assay, NO assay and IL-6 enzyme-linked immunsorbent assay (ELISA). Furthermore, NO levels decreased significantly by 68.2% in the 400 μM EGCG/LPS treated group, while the level of inducible nitric oxide synthase (iNOS) expression decreased by 12-17% in the 200 and 400 μM EGCG/LPS treated group. A significant decrease in transcription of pro-inflammatory cytokines (TNF- α and IL-1β) and anti-inflammatory cytokine (IL-10) was also detected in the EGCG/LPS treated group. However, IL-6 transcript and protein was maintained at a constant level when in the LPS treated group relative to the EGCG/LPS treated group. Overall, these results suggest that the differential regulation of inflammatory cytokines is an important factor influencing the suppressive effects of EGCG against LPS-activated inflammatory response in RAW264.7 cells.

• Journal of Naturopathy
• /
• v.10 no.2
• /
• pp.77-85
• /
• 2021

Background: It is necessary to verify the resilience of the newly improved Kneipp psychotherapy. Purpose: This study assesses whether Kneipp Lebensordnung psychotherapy; KnLP program improves resilience. Methods: The KnLP program, including salutogenesis, logotherapy, meditation, and resilience training, is reorganized in consideration of Korean culture and sentiments. It was conducted 4 times for 25 adults (once a week, 3 hours a time), and data, KRQ-53 (Korean Resilience Quotient-53) measured intervention, before, and after was compared and analyzed. Results: The data for 9 adults were selected to draw reliable analysis, and it concluded that participants' KRQ-53 mean score increased by 14.66 from 191.56 to 206.22 during and after the program. The score increase by factor in resilience training is 5.89 points for self-regulation skills, 4.89 points for in interpersonal skills, and 3.89 points for positive capacity. Conclusions: KnL program improves participants' resilience skill (p<.05), and especially in self-regulation skill. Subsequent studies with more participants are required to achieve statistically significant and generalized results in the future.

• Proceedings of the KIEE Conference
• /
• 2000.11a
• /
• pp.145-148
• /
• 2000

This paper presents new algorithm which is based on LP(Linear Programming) that guarantee convergence. It is considered to minimize generation cost and load shedding as object function subject to various constraints. The proposed algorithm use sensitivity matrix to re-dispatch generation power, so the total CPU time is saved.

• Journal of Convergence Society for SMB
• /
• v.6 no.4
• /
• pp.25-30
• /
• 2016

This paper presents on the development of a compact anti-lost device requested recently. The proposed device consists of the master and slave modules based on Bluetooth4.1 technology. To implement a low-power characteristic, an algorithm has been also developed. The transmitting and receiving circuits are designed by using BoT CLE110 module supporting Bluetooth 4.1. The ATmega 328P-AU was used for the control and LP3874EMP was used as a linear regulator. Power consumption of the fabricated product in operating mode is only 10mAh and 35mAh for MCU operating state. Alarm operation distance is $10m{\pm}30%$, the effective radiated power is less than 10mW, the frequency band is designed to operate in the Bluetooth band with 26MHz bandwidth. And algorithms have been developed to extend the battery life. The size of the product was obtained as $45{\times}45{\times}15mm$ for master and $35{\times}35{\times}10mm$ fr slave. After the optimization process, it is expected to be commercialized as a wristwatch for anti-lost device.

• Microbiology and Biotechnology Letters
• /
• v.49 no.2
• /
• pp.157-166
• /
• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.13 no.5
• /
• pp.2509-2528
• /
• 2019

There are various styles of license plates for different countries and use cases that require style-specific methods. In this paper, we propose and illustrate a multi-style license plate recognition system. The proposed system performs a series of processes for license plate candidates detection, structure classification, character segmentation and character recognition, respectively. Specifically, we introduce a license plate structure classification process to identify its style that precedes character segmentation and recognition processes. We use a K-Nearest Neighbors algorithm with pre-training steps to recognize numbers and characters on multi-style license plates. To show feasibility of our multi-style license plate recognition system, we evaluate our system for multi-style license plates covering single line, double line, different backgrounds and character colors on Korean and the U.S. license plates. For the evaluation of Korean license plate recognition, we used a 50 minutes long input video that contains 138 vehicles of 6 different license plate styles, where each frame of the video is processed through a series of license plate recognition processes. From two experiments results, we show that various LP styles can be recognized under 50 ms processing time and with over 99% accuracy, and can be extended through additional learning and training steps.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.24 no.1
• /
• pp.14-22
• /
• 2022

The purpose of this study is to provide evidence for discovering functional materials through the anti-inflammatory efficacy screening of randomly selected medicinal herbs. We prepared 70% ethanol extracts from 10 herbs and evaluated for the inhibitory effect of NO production on LPS-stimulated mouse macrophage cell line Raw 264.7. As a result, it was confirmed that the Chrysanthemi Zawadskii Herba (CZ) extract had the highest effect of inhibiting NO production induced by LPS. We therefore measured and compared NO inhibitory effects at different concentrations (10, 50, 250 ㎍/mL) of 70% ethanol and water extract of CZ. It was observed that both ethanol and water treatment groups inhibited NO production in a concentration-dependent manner in both ethanol and water treatment groups. In particular, it was confirmed that the CZ 70% ethanol extract (99.97%) had a higher NO inhibitory effect than the water extract (93.32%) in the high concentration (250 ㎍/mL) treatment group. There was no effect of CZ extract on cell viability at all concentrations used in the experiment. Moreover, it was shown that CZ ethanol extract remarkably inhibited the expression of VCAM-1 induced by TNF-𝛼, and it was slightly decreased even by treatment with water extract. This study suggests that Chrysanthemi Zawadskii Herba has potential as a functional substance that regulates vascular inflammation.