한국축산식품학회지 (Food Science of Animal Resources)

  • 제38권5호
  • /
  • Pages.878-888
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  • 2018
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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Comparative Genome Analysis and Evaluation of Probiotic Characteristics of Lactobacillus plantarum Strain JDFM LP11

  • Heo, Jaeyoung (International Agricultural Development and Cooperation Center, Chonbuk National University) ;
  • Shin, Donghyun (Department of Animal Biotechnology, Chonbuk National University) ;
  • Chang, Sung Yong (Department of Animal Science and Institute of Milk Genomics, Chonbuk National University) ;
  • Bogere, Paul (Graduate School of Agricultural Convergence Technology, Chonbuk National University) ;
  • Park, Mi Ri (Department of Animal Science and Institute of Milk Genomics, Chonbuk National University) ;
  • Ryu, Sangdon (Department of Animal Science and Institute of Milk Genomics, Chonbuk National University) ;
  • Lee, Woong Ji (Department of Animal Science and Institute of Milk Genomics, Chonbuk National University) ;
  • Yun, Bohyun (Department of Animal Science and Institute of Milk Genomics, Chonbuk National University) ;
  • Lee, Hak Kyo (Department of Animal Biotechnology, Chonbuk National University) ;
  • Kim, Younghoon (Department of Animal Science and Institute of Milk Genomics, Chonbuk National University) ;
  • Oh, Sangnam (Department of Functional Food and Biotechnology, Jeonju University)
  • 투고 : 2018.05.28
  • 심사 : 2018.07.24
  • 발행 : 2018.10.31
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초록

In the current study, the probiotic potential of approximately 250 strains of lactic acid bacteria (LAB) isolated from piglet fecal samples were investigated; among them Lactobacillus plantarum strain JDFM LP11, which possesses significant probiotic potential, with enhanced acid/bile tolerance, attachment to porcine intestinal epithelial cells (IPEC-J2), and antimicrobial activity. The genetic characteristics of strain JDFM LP11 were explored by performing whole genome sequencing (WGS) using a PacBio system. The circular draft genome have a total length of 3,206,883 bp and a total of 3,021 coding sequences were identified. Phylogenetically, three genes, possibly related to survival and metabolic activity in the porcine host, were identified. These genes encode p60, lichenan permease IIC component, and protein TsgA, which are a putative endopeptidase, a component of the phosphotransferase system (PTS), and a major facilitator in the gut environment, respectively. Our findings suggest that understanding the functional and genetic characteristics of L. plantarum strain JDFM LP11, with its candidate genes for gut health, could provide new opportunities and insights into applications in the animal food and feed additive industries.

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