• The Plant Pathology Journal
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• 제32권1호
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• pp.65-69
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• 2016

In plant virus ecology, weeds are regarded as wild reservoirs of viruses and as potential sources for insect-mediated transmission of viruses. During field surveys in 2013-2014, three Leonurus sibiricus plants showing virus-like symptoms were collected from pepper fields in Daegu, Seosan, and Danyang in Korea. Molecular diagnosis assays showed that the collected L. sibiricus samples were infected with either Tomato spotted wilt virus (TSWV), Pepper mild mottle virus (PMMoV), or Beet western yellow virus (BWYV), respectively. Since this is the first identification of TSWV, PMMoV, and BWYV from L. sibiricus, complete genome sequences of three virus isolates were determined to examine their phylogenetic relationships with the previously reported strains and isolates. Phylogenetic analyses performed using full genome sequences of the viruses showed the isolates of TSWV and PMMoV obtained from L. sibiricus are closely related to the pepper isolates of the corresponding viruses. Our results suggest that L. sibiricus could act an alternative host and reservoir of viruses that cause damages in pepper fields.

• 한국식품저장유통학회지
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• 제20권4호
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• pp.592-596
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• 2013

익모초 가공을 위한 기본 추출조건을 확립하기 위하여 3가지 조건으로 익모초 물추출물(열수추출물(I), 고온고압-열수추출물(II), 습식분쇄-열수추출물(III))을 제조하고 이들의 생리적 특성을 비교하였다. 각 추출조건에 대한 수율은 13.02~15.90%로 유의적인 차이가 없었고, 총 폴리페놀 함량은 추출물(II)와 (III)에서 유의적으로 높았다. 전자 공여능에 대한 $IC_{50}$은 추출물(II)에서 가장 낮았고 그 뒤로 추출물(III) < 추출물(I)순으로 유의적인 차이를 나타내었으며, ABTS 라디칼 소거활성은 추출물(II)와 (III)에서 유의적으로 높았다. 아질산염 소거능은 추출조건간 유의적인 차이가 없었으며, ACE저해 활성은 고농도(5 mg/mL)의 추출물에 대해 추출물(I)보다 추출물(II)와 (III)에서 유의적으로 높았다.

• 한국가축번식학회지
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• 제19권4호
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• pp.245-250
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• 1996

This study was undertaken to illustrate the uterotonic effect of Leonurus sibiricus. It was dissolved in sterile water and several different dosages were administered both in vitro and in vivo study. Rat uterine tissue for in vitro bioassay was obtained from estrous rat. From the low to high dosages of Leonurus sibiricus were tried and each uterine contraction was recorded and integrated. Anesthetized estrous rat for in vivo study was cannulated into the jugular vein for infusion of the compound. Another cannula with a balloon tipped and water filled was inserted into the uterus to measure uterine activity. While the uterine tissue did not respond to low dosage of compound, high dosage of compound stimulated the tissue to contract less than 1 minute with low amplitude. In vivo rat uterus showed a certain, consistent pattern of contractions which was initial relaxation and followed by prolonged and increased amplitude of contractions. It also caused a short breathing stop which might be due to acute acidosis.

• 한국식품영양학회지
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• 제9권3호
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• pp.294-298
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• 1996

By the 505 chromotest which utilized Escherichia bolt PQ 37, Korean medicinal plants had been screened to Investigate the antimutagenic effect to aflatoxin B1(AFBl). Ikmocho(IMC, Leonurus sibiricus L.) was extracted with hot water. The extract was not found to be mutagenic in the Salmonella mutation test with or without metabolic activation, and the extract was showed to possess the antimutagenic properties towards AFB1-induced metation. The mutagenicity of AFB1 was inhibited by methanol soluble fracstion (IMC-MS) in dose-dependent. However, water-soluble fraction exhibited comutagenic activity. The greatest inhibitory effect of IMC-MS on AFB1 mutagenicity occurred when IMC-MS was first incubated, AFB1 followed by a second incubation with the cells and 59 mixture. Also lower inhibition was occurred when S9 mixtures were first incubated, with IMC-MS followed by a second incubation with AFBI. The results of the sequential incubation study support the probability that one mechanism of inhibition could involve the formation of chemical complex between IMC-MS and AFB1 rather than deactivation of S9 enzyme.

• 한국식품영양과학회지
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• 제16권1호
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• pp.22-28
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• 1987

These studies were conducted to investigate the effects of Leonurus sibiricus extract (L.S.E.) on experimentally $CCl_4-induced$ liver damage and alloxan-induced diabetes in rabbits; Leonurus sibiricus juice (L.S.J.) showed the most rapid recuperation of liver function among samples compared to the control group in $CCl_4-intoxicated$ rabbits. SGPT activity stowed an apparant decreasing effect in 4 days, and the blood level of total cholesterol and total bilirubin in 6 days, and not in alkaline phosphatase level. L.S.J. exhibited more excellent hypoglycemic effect in alloxan-induced diabetes of rabbits, that is, significant to the control group after 2 days and adjacent to the normal level on 8th day. And SGPT activity and total cholesterol level were gradually decreased and showed markedly decreasing effect after 4 days.

• 생약학회지
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• 제38권2호통권149호
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• pp.148-151
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• 2007

Ig Mo Cho (益母草) has been used as remedy for invigorating blood circulation to remove blood stasis and relieving edema by inducing diuresis. With regard to the botanical origin of Ig Mo Cho, it has been considered to be Leonurus spiecies of Labiatae, but there has never been pharmacognostical confirmation on it. To clarify the botanical origin of Ig Mo Cho from Korea, the anatomical characteristics of Leonurus japonicus and L. sibiricus were studied. As a result, the botanical origin of Ig Mo Cho from Korea was proved the herba of Leonurus japonicus.

• 식물병연구
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• 제8권2호
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• pp.113-116
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• 2002

A sclerotinia rot of Leonurus sibiricus caused by Sclerotinia sclerotio겨m occurred severely in farmer's fields at Seosang-myon, Hamyang-gun, Gyeongnam Province, Korea in 2001. The typical symptoms of the disease were that leaves, stems or collar of the infected plants at first became darker green and then watery soft rotted, and white fluffy mycelia formed on the lesion developed into black sclerotia later, Sclerotia on the plant and PDA medium were globose to cylindrical or irregular in shape, and 0.8~10.3$\times$0.8~6.4 m in size. Aphothecia with numerous asci were cup-shape, and 0.6~1.2 cm in diameter, Asci with 8 spores were cylindrical, and 87~246$\times$4.6~21.6 $\mu\textrm{m}$ in size. Ascospores of one cell were hyaline, ellipsoid to ovoid in shape, and 10.2~14.6$\times$4.7~7.2 $\mu\textrm{m}$ in size. The range of temperature for mycelial growth was from 5 to 3$0^{\circ}C$, and the optimum was $25^{\circ}C$. This is the first report on the sclerotinia rot of L. sibiricus caused by Sclerotinia sclerotioum in Korea.

• 동의생리병리학회지
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• 제30권2호
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• pp.81-87
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• 2016

This study was performed to investigate the cholesterol-lowering effect of liquid extract from Leonurus sibiricus Linne (LS). SD rats were fed atherogenic diet for 6 weeks. During the experimental period, LS liquid extract (3 g/kg and 6 g/kg) was orally given to the rats. The liver weight and levels of total cholesterol, LDL-cholesterol, and triglycerides in serum were significantly increased in rats fed with atherogenic diets. Oral treatment of LS liquid extract to the rats fed atherogenic diet significantly reduced the liver weights and significantly decreased the levels of total cholesterol, LDL-cholesterol, and triglycerides. The level of serum HDL-cholesterol was significantly increased at a high dose group compared with rats fed normal diet chow. These results indicate that LS liquid extract has the potential to restore altered lipid profiles induced by cholesterol rich diets and prevent the development of atheroclerosis.

• 생약학회지
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• 제32권4호통권127호
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• pp.322-326
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• 2001

For the quality control of Leonuri Herba, alkaloid compound, leonurine, was isolated from the MeOH extract of Leonurus sibiricus L. (Labiatae) and identified by the spectroscopic analysis. A quantitative analysis of leonurine using HPLC method showed that the average contents were $0.124{\pm}0.065%$ in 34 samples collected throughout the regions of Korea.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2012년도 심포지엄 및 춘계학술발표회
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• pp.275-276
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• 2012