• Title/Summary/Keyword: Leaf water content

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Physiological Activities of Extracts of Cedrela sinensis leaves (참죽나무 잎 추출물의 생리활성)

  • Shin, Hee-June;Jeon, Young-Jin;Shin, Hyun-Jae
    • KSBB Journal
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    • v.23 no.2
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    • pp.164-168
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    • 2008
  • The purpose of this study was to confirm the content of total polyphenol, antioxidative and immune activities of the extracts of Cedrela sinensis leaf. The content of total polyphenol of water extracts ranged from 46.5-59.6 mg/100 g, which was higher than other extracts using organic solvents such as EtOAc, $CH_2Cl_2$ and $C_6H_{14}$. The antioxidant activity of the water and organic solvents extracts showed 6-33% in terms of 2,2-diphenyl-picryl-hydrazyl (DPPH) scavenging activity. To analyze the immuno-stimulation activity of C. sinensis leaf extract, we investigated the effect of the extracts on NO synthesis which is important in host defense against bacterial infection. Hot water extracts significantly increased NO generation by RAW 264.7, macrophage cell line, while organic solvent extract has no significant effect on NO production. To further analyzed the anti-inflammatory effect of the extracts, we investigated the effects of the extracts on lipopolysaccharide(LPS)-induced NO generation. Organic solvent extracts of C. sinensis leaves showed strong inhibitory effect on NO production in LPS-stimulated RAW 264.7 cells. These results suggest that C. sinensis leaf extract may represent a useful immune stimulating agent and anti-inflammatory agent.

The research on antioxidative effect of Sasa quelpaertensis extractum and assessment of cytotoxicity (제주조릿대 잎 추출물의 항산화효능 및 세포독성 평가에 관한 연구)

  • Lee, Song Hee;Lee, Moo Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.687-693
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    • 2017
  • This research analyzed cell survival rate according to antioxidative effect and cytotoxicity using Sasa quelpaertensis and Ethanol extracts. Sasa quelpaertensis used extracts of $95^{\circ}C$ boiled-water extract and 70% ethanol liquid each. For total polyphenol content, tannin content, and flavonoid content, polyphenol content of $95^{\circ}C$ boiled-water extract was 26.6 mg/g while that of 70% ethanol extract liquid was 22.3 mg/g, which means polyphenol content was higher in $95^{\circ}C$ boiled-water extract. Tannin content was 72.1 mg/g in $95^{\circ}C$ boiled-water extract which was higher than 61.2 mg/g in 70% ethanol extract liquid. Total flavonoid content was higher in 70% ethanol extract liquid (25.4 mg/g) than in $95^{\circ}C$ boiled-water extract (17.8 mg/g). Further, the researcher measured DPPH radical elimination and ABTS radical elimination. As a result, as the concentration of each extract increased, DPPH radical elimination and ABTS radical elimination increased. In the DPPH radical elimination, L-ascorbic acid was eliminated at 500 ppm and showed no change even though the concentration increased, whereas it increased in BHT and Sasa quelpaertensis leaf extracts according to concentration. ABTS radical elimination indicated the similar phenomenon, whereas BHT showed maximum elimination at 500 ppm and decreased gradually as the concentration increased. On the other hand, it gradually increased in Sasa quelpaertensis leaf extract and showed 90% elimination at 10,000 pm, and it increased in $95^{\circ}C$ boiled-water extract. Using human skin fibroblasts (3T3) to check the cell survival rate of Sasa quelpaertensis leaves showed that cell survival rate decreased between 85.6 and 66.6% in $95^{\circ}C$ boiled-water extract. The cell survival rate was between 95.4 and 92.1% when using 70% ethanol extracts. Sasa quelpaertensis has efficacy as a natural antioxidant as well as a material hyangjang.

Effect of Soil Water Content on Growth, Photosynthetic Rate, and Stomatal Conductance of Kimchi Cabbage at the Early Growth Stage after Transplanting (정식 후 초기 생장기 배추의 생장, 광합성 속도 및 기공전도도에 미치는 토양수분의 영향)

  • Kim, Sung Kyeom;Lee, Hee Ju;Lee, Hee Su;Mun, Boheum;Lee, Sang Gyu
    • Journal of Bio-Environment Control
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    • v.26 no.3
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    • pp.151-157
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    • 2017
  • The objectives of this study were to determine the impact of soil water content on the growth, stomatal conductance, and photosynthesis of Kimchi cabbage and to evaluate proper parameters for development of growth models. There were five levels of irrigation amount treatments (0, 200, 300, 400, and 500 mL/d/plant) and those were commenced at one day after transplanting (DAT). We measured soil water content, stomatal conductance, photosynthesis characteristics, and the A-Ci curve. The growth of Kimchi cabbage as affected by irrigation amount was evaluated at 38 days after transplanting, however, the growth with 0 and 200 mL/d/plant irrigation amount treatments measured at 29 DAT. The relationship between soil water content and stomatal conductance was highly correlated ($r^2=0.999$) and the function represented by y = 6097.4x - 4.2984. The stomatal conductance of Kimchi cabbage leaves showed $300mmol{\cdot}m^{-2}{\cdot}s^{-1}$ when the soil water content was below $0.05m^3/m^3$. The stomatal conductance was rapidly decreased by scarcity of soil moisture. A-Ci curve indicated normal curve in fully irrigation treatment (500 mL/d/plant), however, $CO_2$ couldn't diffuse through the intercellular Kimchi cabbage leaves treated with 0 mL/d/plant. The dry weight of full irrigation treatment was greater approximately 6.8 times than that of deficit irrigation (0 mL/d/plant). In addition, leaf area index showed a logarithmic function (y = 16.573 + 3.398 ln x) with soil water content and that of R-squared represents 0.913. Results indicated that the soil water content was highly correlated with stomatal conductance and leaf area index. Indeed, the scarcity soil moisture reduced photosynthesis and retarded growth.

Growth Dynamics and Carbon Incorporation of the Seagrass, Zostera marina L. in Jindong Bay and Gamak Bay on the Southern Coast of Korea (진동만과 가막만에 서식하는 잘피 개체군의 생장 동태 및 탄소고정량 추정)

  • Kim, Tae-Hwan;Rark, Sang-Rul;Kim, Young-Kyun;Kim, Jong-Hyeob;Kim, Seung-Hyeon;Kim, Jeong-Ha;Chung, Ik-Kyo;Lee, Kun-Seop
    • ALGAE
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    • v.23 no.3
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    • pp.241-250
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    • 2008
  • Since seagrasses in the coastal and estuarine ecosystems achieve high levels of production, they require high inorganic carbon and nutrient incorporation. Thus, seagrasses may play a significant role in carbon and nutrient cycling in the coastal and estuarine ecosystems. To examine growth dynamics of Zostera marina L. environmental factors such as underwater irradiance, water temperature, and salinity, and biological parameters such as shoot density, biomass, shoot morphology, and leaf productivity were measured in two bay systems (Jindong Bay and Gamak Bay) on the southern coast of Korea. While underwater irradiance did not show distinct seasonal trend, water temperature at both sites exhibited clear seasonal trend throughout the experimental period. Shoot density increased dramatically during winter due to the increased seedlings through germination of seeds in Jindong Bay and due to the increased lateral shoots in Gamak Bay. Eelgrass biomass increased during winter and decreased during summer. Maximum biomass in Jindong Bay and Gamak Bay was 250.2 and 232.3 g dry weight m–a2, respectively. Carbon incorporation into the eelgrass leaf tissues was estimated from productivity and leaf tissues carbon content. The calculated annual carbon incorporations at the Jindong Bay and Gamak Bay sites were 163 and 295 g C m–`2 y–`1, respectively. This high carbon incorporation into seagrass tissues suggests that seagrass habitats play an important role as a carbon absorber in the coastal and estuarine ecosystems.

Antioxidative Activity of Ethanol Extract from Lotus (Nelumbo nucifera) Leaf (연잎 에탄올 추출물의 항산화 효과)

  • Lee, Kyung-Seok;Kim, Min-Gue;Lee, Ki-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.2
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    • pp.182-186
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    • 2006
  • This study was investigated on the antioxidative activity of $70\%$ ethanol extracts from lotus (Nelumbo nucifera) leaf. The extraction yields of $70\%$ ethanol extract was $8.16\%$. The extract was further fractionated subsequently by hexane, chloroform, ethyl acetate, butanol and water. Antioxidative activity was examined by electron donating ability (EDA) using DPPH, TBA value, acid value (AV), and peroxide value (POV), in comparison with commercial antioxidants. Butanol fraction showed the highest extraction yields but ethyl acetate fraction showed the highest phenol content. The ethyl acetate fraction showed the highest EDA. The antioxidative activity of ethyl acetate fraction determined by AV and POV in accelerated oxidation condition of lard was similar to that of BHA.

The Application of Satellite Data to Land Surface Process Parameterization in ARPS Model (ARPS 모형 지면 과정 모수화에 위성 자료의 응용)

  • Ha, Kyung-Ja;Suh, Ae-Sook;Chung, Hyo-Sang
    • Journal of the Korean Association of Geographic Information Studies
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    • v.1 no.1
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    • pp.99-108
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    • 1998
  • In order to represent the surface characteristics in local meteorological model, soil type, vegetation index, surface roughness length, surface albedo and leaf area index should be prescribed on the surface process parameterization. In this study, the $1^{\circ}/1^{\circ}leaf$ area index, surface roughness length, and snow free surface albedo and fine mesh NDVI with seasonal variation derived from the satellite observation were applied to the land surface process parameterization. From comparison between with and without satellite data in the interactions between biosphere and atmosphere, land and atmosphere, the sensitivity of the simulated heat, energy and water vapor fluxes, ground temperature, wind, canopy water content, specific humidity, and precipitation fields were investigated.

Development of Leaf Protein Concentrates II. Extraction of Leaf Protein Concentrates of Some Plants Growing in Korea (잎 단백질(蛋白質)(Leaf Protein Concentrates)의 개발(開發)에 관한 연구(硏究) II. 한국산(韓國産) 각종 식물(植物)로 부터의 잎 단백질(蛋白質)의 추출(抽出))

  • Choe, Sang;Kim, Geon-Chee;Chun, Myung-Hi;Kim, Kil-Hwan
    • Korean Journal of Food Science and Technology
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    • v.2 no.2
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    • pp.17-25
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    • 1970
  • Juice were extracted from fresh leaves of 70 species of plants growing in Korea by mincing and pressing the resulting pulp through a cotton cloth. Leaf protein concentrates could be prepared from many species of land and water plants that are at present economically unimportant. The choice of plants is of considerable important. Total-N, protein-N and pH determinations were made on the extracts, and total-N remaining in the fibre were calculated. Leaf protein concentrates were precipitated from the extracts at $75{\sim}80^{\circ}C$, and analysed total-N as protein-N of products. The present paper deals with the calculated yields of leaf protein concentrates from various plants, relations between yield of leaf protein concentrates and total-N of leaves, or pH of extracts, and the amino acid compositions of leaf protein concentrates. Results are summarized as follows. 1. Spinach and radish were the best sources of easily extractable, but good results were also obtained with indian mustard, kail, chenopod, red bean, cucumber, squash, houndberry, white flowered gourd, potato, Humulus japonicus, arrowroot and soybean as a good resources for the production of leaf protein concentrates. 2. In general, the greater the protein content of leaves the greater the yield of leaf protein concentrates. However, there are some plants difficult to make a adequate protein extraction by a simple mechanical process. 3. It was to be expected that leaf protein concentrates would be more extractable with the higher pH of extracts. There were a poor yield of the leaf protein concentrate in the pH values lower than 5.50 of the first extracts. 4. Protein content of the leaf protein concentrate shows marked differences, depending on species and season. It ranged between 29 to 80% of protein contents. However, the majority of plants yielded products containing more than 50% of protein. Products containing more than 75% of protein were obtained from two species of radish and indian mustard. Cabbage and Digitaria sanguinalis cilialis (summer) made products containing 29 to 32% of protein. 5. The amino acid composition of leaf protein concentrates was not greatly altered by species of plants. On an amino acid compositional basis, the leaf protein concentrate has a favorable balance of essential and non-essential amino acids, the only exception being methionine, which was usually low in all cases.

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Analysis of Carbon Emissions from Combustion of Three Arbor in Youngdong Area (영동지역 교목 3수종 생엽의 연소에 따른 탄소배출량 분석)

  • Park, Young-Ju;Lee, Hae-Pyeong
    • Journal of the Korean Society of Safety
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    • v.25 no.6
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    • pp.210-215
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    • 2010
  • In this study, when the forest fire occurred, in order to estimate greenhouse gas emissions, tree glow in Gangwon Youndong area, Juglans mandshurica, Alnus japonica, Acer palmatum of carbon dioxide and carbon monoxide emissions were about. Water content were measured before the experiment, Juglans mandshurica 196.24%, Alnus japonica 169.17% Acer palmatum 210.10% moisture content showed a big difference, Living leaves of Acer palmatum were a lot of moisture. Also, 50g weight of carbon dioxide on the Juglans mandshurica 53.3644g, Alnus japonica 49.4256g, was released about Acer palmatum 51.3394g, Juglans mandshurica living leaves were the most carbon dioxide emissions. Carbon monoxide emissions result, About weight 50g Juglans mandshurica 1.5329g, Alnus japonica 1.7189g, 2.5002g about Acer palmatum was released, Acer palmatum living leaves were the most carbon monoxide emissions.

Antioxidant Activities of Different Parts of Synurus deltoids Nakai Extracts in Vitro

  • Jung, Mee-Jung;Heo, Seong-Il;Wang, Myeong-Hyeon
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1156-1159
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    • 2008
  • The antioxidant activity of hot water extracts of various parts, the leaf, stem, and root of Synurus deltoides was evaluated by various antioxidant assays, including total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical (${\cdot}OH$) scavenging, superoxide dismutase (SOD), and xanthine oxidase (XOI) activities. The various antioxidant activities were compared with the standard antioxidants such as L-ascorbic acid, $\alpha$-tocopherol, and butylated hydroxyanisole (BHA). Among the different plant parts, stem has been found to possess the highest activity in all tested model systems, the activity decreased in the order stems>roots>leaves. These results indicate that stem extract could be used as potential source of natural antioxidant.

Decomposition of leaf litter of some evergreen broadleaf trees in Korea

  • Lee, Kyung Eui;Cha, Sangsub;Lee, Sang Hoon;Shim, Jae Kuk
    • Journal of Ecology and Environment
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    • v.38 no.4
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    • pp.517-528
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    • 2015
  • Litter decomposition is an important process in terrestrial ecosystem. However, studies on decomposition are rare, especially in evergreen broadleaf trees. We collected the leaf litter of five evergreen broadleaf trees (Daphniphyllum macropodum, Dendropanax morbifera, Castanopsis cuspidata var. thunbergii, Machilus thunbergii and Quercus acuta), and carried out a decomposition experiment using the litterbag method in Ju-do, Wando-gun, Korea for 731 days from December 25, 2011 to December 25, 2013. Among the five experimental tree species, C. cuspidata var. thunbergii distribution was limited in Jeju Island, and D. macropodum was distributed at the highest latitude at Mt. Baekyang (N 35°40′). About 2% of the initial litter mass of D. macropodum and D. morbifera remained, while 20.9% remained for C. cuspidata var. thunbergii, 30.4% for M. thunbergii, and 31.6% for Q. acuta. D. macropodum litter decayed four times faster (k = 2.02 yr-1) than the litter of Q. acuta (k = 0.58 yr-1). The decomposition of litter was positively influenced by thermal climate such as accumulated mean daily air temperature (year day index) and precipitation, as well as by physical characteristics such as thickness (R2=0.939, P = 0.007) and specific leaf area (SLA) (R2 = 0.964, P = 0.003). The characteristics of chemical composition such as lignin (R2 = 0.939, P = 0.007) and water-soluble materials (R2 = 0.898, P = 0.014) showed significant correlations with litter decomposition. However, the nutrients in litter showed complicated species-specific trends. The litter of D. macropodum and D. morbifera had fast decomposition despite their low nitrogen concentration and high C/N ratio. This means that the litter decomposition was more strongly affected by physical characteristics than chemical composition and nutrient content. On the other hand, the litter of Q. acuta which had the slowest decay rate had a high amount of N and low C/N ratio. Thus, the decomposition of Q. acuta litter was more affected by the P content of the litter than the N content, although all litter had similar physical characteristics.