• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.493-496
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• 2016

Peach rosette mosaic virus (PRMV) is a plant virus that was first reported in 1933 by Peach. It can infect hosts including peach, grape, blueberry, dandelion, plum, cherry tree, and weeds. PRMV is non-reportable in Korea, but it is designated as a controlled virus requiring plant quarantine. In this study, for the rapid and specific detection of PRMV, we developed an assay using loop-mediated isothermal amplification (LAMP). Comparison between conventional polymerase chain reaction (PCR) methods (real time-PCR and nested PCR) and LAMP for the detection of PRMV revealed an equivalent level of sensitivity by all the tested methods. For the LAMP assay, outer primer sets were used to amplify a 264-bp PCR product, which was then digested using the restriction enzyme Pvu II (CAG/CTG), and the visualization of two digestion fragments (207 + 57 bp) indicated a positive reaction. The developed LAMP assay for PRMV is expected to enable the rapid monitoring of PRMV in plants.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.711-720
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• 2009

Characteristics of spectral irradiance based on the distance from the light source, which combined metal halide lamp and high-luminance light-emitting diode (LED) light, were studied to investigate a suitable operating method for fishing lamps of the next generation. A 380-780 nm wavelength radiation was superior when using 1 W electrical power in the order of metal halide lamp, blue LED, white LED, and combined LED lights. The wavelengths at which the irradiance was at a maximum were fixed to 581 nm for the light source, which was combined for each ratio. If the irradiance characteristics at 300-1100 nm wavelengths were set as 100%, the irradiance rates at 400-599 nm were 100%, 72.7%, 88.9%, and 69.5% for the blue, white, combined LED lights, and metal halide lamp, respectively. This indicated that the color rendering of the LED lights was dependent on the metal halide lamp light source. When the horizontal profiles (450-550 nm wavelength) of irradiances were compared to a different type of light source in the ratio white LED: combined LED lights: blue LED: metal halide lamp, the irradiated area of more than $0.01\;{\mu}mol/s/m^2/nm$ was in the ratio 1.0 : 1.3 : 1.7 : 37.3, respectively. Based on the radiation characteristics and irradiance according to the distance from the light source, LED lights have an estimated economic efficiency if used before and after operation of a metal halide lamp.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.9
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• pp.1326-1330
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• 2012

This study was conducted to develop a loop-mediated isothermal amplification (LAMP) method for the detection of Clostridium difficile. The tested target gene was 16S ribosomal RNA. Five different LAMP primer sets were designed, and LAMP was performed. All primer sets targeting the 16S rRNA gene (BIP, FIP, B3, F3, LF, PF) were determined as positive in tcdA-positive, tcdB-postive ($A^+B^+$) and tcdA-negative, tcdB-negative ($A^-B^-$) Clostridium difficile strains. As the LAMP reaction took less than 80 min and did not require expensive machine such as thermocycler, it can be used as a rapid and simple detection method for foodborne pathogens.

• Fire Science and Engineering
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• v.20 no.1 s.61
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• pp.43-49
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• 2006

In this paper, we are studied on the temperature characteristics and fire progress of an incandescent electric lamp at 220V/100W. In the case of stationary state, the ignition possibility of the incandescent electric lamp due to the heat generation was low because the temperature was measured at $161.9^{\circ}C$ the temperature was increased at $538.1^{\circ}C$ in the airtight chamber, but it does not generated the fire because the oxygen was not exist in the airtight chamber. When the lamp is broken, the filament of lamp was melted in the air. The gas of lamp interior spurted to the weakest part by external flame. Thus, the incandescent electric lamp is high possibility of fire when oxygens from airtight space. Also, it is known that the possibility of ignition is very high if combustion materials(sawdust) exists on surrounding. These experimental results will be utilized for the data in the investigation electrical fire cause.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.3
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• pp.1265-1271
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• 2014

The lamp cooling system of the exposure has effect on the exposure efficiency and device lifetime. In this paper, we performed the numerical analysis about the thermal flow in the lamp housing of the exposure apparatus for the cooling air inflow rate. We set up the velocity of cooling air of side and bottom as the independent variables because cooling performance of the lamp housing is affected by the velocity of the cooling air side and bottom. The cooling state of lamp housing depend on three dependent variables; the temperature at top mirror and exhaust gas, ellipsoidal mirror. Response surface methodology was used in order to establish the efficient cooling analysis plan. The regression equation predicting the variables temperature of lamp housing according to the cooling air velocity were drawn. The velocity of cooling air to reach the optimum temperature of the lamp housing were derived.

• Journal of Biosystems Engineering
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• v.23 no.6
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• pp.591-598
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• 1998

Light intensity and spectral characteristics of different types of fluorescent lamps were tested to investigate their possibility as the artificial lighting sources for the close illumination applied in the transplant production factory. Photosynthetic photon flux densitiy(PPF), illuminance and irradiance for all lamps decreased logarithmically with an increase of the vertical distance from the lighting source. The fluorescent lamp specially designed plant growth (PG lamp) showed a maximum spectral irradiance at the wavelength of 660nm. However, it showed lower irradiance than that of a standard fluorescent lamp at the range of wavelength between 500 and 600nm. On the other hand, PG lamp showed higher PPF and lower illuminance than those of the standard fluorescent lamp. The maximum peak of spectral characteristics for both of the single and twin three-bind fluorescent lamps was shorn at the wavelength of 545m and the next peaks were shown at the wavelength of 610nm and 435nm, respectively. Since the red fluorescent lamp has a narrower peak at the wavelength of 660nm, it may be useful for the supplementary red lighting. For three of standard, single three-band and twin three-band fluorescent lamps, the values of conversion factor for converting illuminance to PPF fell within the narrow range from 76 to 791x/$\mu$molㆍm$^{-2}$ ㆍs$^{-l}$ . However, for PG lamp, it was 29.71x/$\mu$molㆍm$^{-2}$ ㆍs$^{-1}$. Also, the values of conversion factor for converting PPF to irradiance of fluorescent lamp used in this study ranged between 4.85 and 5.34$\mu$molㆍm$^{-2}$ ㆍs$^{-1}$/Wㆍm$^{-2}$ .

• Resources Recycling
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• v.15 no.6 s.74
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• pp.25-32
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• 2006

In this study, waste car tau lamps that usually gets incinerated or buried has been recovered during the dispose process in order to increase the recycling rate of waste cars and the material separation research was performed by applying triboelectrostatic separation method. After a series of charging characteristic test, PMMA materials was confirmed of being an effective charging material for waste car tail lamp material separation, a cyclone charger using PMMA material for continuous process was developed. In optimum test conditions using triboelectrostatic separator developed in this study, high quality PMMA material with recovery rate of 99.0% and 90.2% was achieved from the waste car tail lamp. Therefore, a material separation technology that can recycle tail lamps of waste cars has been established.

• Journal of the Korean Society of Safety
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• v.29 no.4
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• pp.48-53
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• 2014

This study is analyzed for electrical fire danger by electric equipments through surveys of stable owners and the field investigation at 64 stable. Also, an experiment is performed to analyze the thermal characteristics of 4 types electric wiring according to the number of operating halogen warm lamp group and automatic waterer group in Beon-young stable in the Cheongwon location. The survey is done to investigate the occurrence of electrical fire, electric equipment, causes and dangerous equipment. As a result, halogen warm lamp group and automatic waterer group are found to have dangerous loads. In the field investigation, the mode of load capacity of the halogen warm lamp group and automatic waterer group was investigated. The mode of load capacity of halogen warm lamp group (over 3 years) is found 17A and that of the auto waterer group (10 years) is 16A. In this experiment, the halogen warm lamp group aged at least 3 years showed $109^{\circ}C$ while that aged less than 1 year appeared $83^{\circ}C$, showing the max gap of $26^{\circ}C$. The automatic waterer group aged 10 years showed $90^{\circ}C$ while that aged less than 1 year appeared $68^{\circ}C$, showing the max gap of $22^{\circ}C$. These findings indicate that the mode of load capacity needs to be considered in selecting and installing wiring for the electric equipment construction of halogen warm lamp group and automatic waterer group in stable.

• Biomedical Science Letters
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• v.25 no.1
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• pp.75-82
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• 2019

We developed the multiplex LAMP assay using 16S rRNA, femA and mecA genes for direct detection of the methicillin resistance in Staphylococci from positive blood culture. To simultaneously recognize Staphylococci genus, S. aureus and methicillin resistance, three sets of six primers for 16S rRNA, femA and mecA were designed, respectively. The performance of LAMP assay was affirmed using VITEK system for the phenotypic methods of identification and for oxacillin and cefoxitin antimicrobial susceptibility. The optimal condition for LAMP assay was obtained under $64^{\circ}C$ for 50 min. The detection limit was determined to be of 20 copies and CFU/reaction ($10^4CFU/mL$). For clinical application of comparison with phenotypic methods, the sensitivity and specificity of the LAMP with femA gene for detecting S. aureus was 95.31% and 100%, respectively. The sensitivity and specificity of the LAMP with mecA gene for detecting methicillin resistance was 98.46% and 100%, respectively. The multiplex LAMP assay with femA and mecA gene successfully detected all of MRSA (38 isolates) isolates from 103 Staphylococci in blood cultures. The LAMP assay developed in this study is sensitive, specific, and of excellent agreement with the phenotypic methods.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.454-464
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• 2019

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, $MgSO_4$ concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as $89fg/{\mu}l$, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.