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http://dx.doi.org/10.4014/mbl.1609.09004

Development of a Rapid Assay for Peach Rosette Mosaic Virus Using Loop-mediated Isothermal Amplification  

Lee, Siwon (Environmental Infrastructure Research Department, National Institute of Environmental Research)
Lee, Jin-Young (Department of Microbiology, College of Natural Sciences, Dankook University)
Kim, Jin-Ho (Department of Chemistry, College of Natural Science, Dankook University)
Rho, Jae-Young (Department of Microbiology, College of Natural Sciences, Dankook University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.4, 2016 , pp. 493-496 More about this Journal
Abstract
Peach rosette mosaic virus (PRMV) is a plant virus that was first reported in 1933 by Peach. It can infect hosts including peach, grape, blueberry, dandelion, plum, cherry tree, and weeds. PRMV is non-reportable in Korea, but it is designated as a controlled virus requiring plant quarantine. In this study, for the rapid and specific detection of PRMV, we developed an assay using loop-mediated isothermal amplification (LAMP). Comparison between conventional polymerase chain reaction (PCR) methods (real time-PCR and nested PCR) and LAMP for the detection of PRMV revealed an equivalent level of sensitivity by all the tested methods. For the LAMP assay, outer primer sets were used to amplify a 264-bp PCR product, which was then digested using the restriction enzyme Pvu II (CAG/CTG), and the visualization of two digestion fragments (207 + 57 bp) indicated a positive reaction. The developed LAMP assay for PRMV is expected to enable the rapid monitoring of PRMV in plants.
Keywords
LAMP; Loop-mediated isothermal amplification; Peach rosette mosaic virus; PRMV; rapid detection;
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Times Cited By KSCI : 3  (Citation Analysis)
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