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http://dx.doi.org/10.3746/jkfn.2012.41.9.1326

Detection of Clostridium difficile by Loop-Mediated Isothermal Amplification  

In, Ye-Won (Dept. of Food and Nutrition, Kookmin University)
Ha, Su-Jeong (Dept. of Food and Nutrition, Kookmin University)
Yang, Seung-Kuk (Dept. of Food and Nutrition, Kookmin University)
Oh, Se-Wook (Dept. of Food and Nutrition, Kookmin University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.41, no.9, 2012 , pp. 1326-1330 More about this Journal
Abstract
This study was conducted to develop a loop-mediated isothermal amplification (LAMP) method for the detection of Clostridium difficile. The tested target gene was 16S ribosomal RNA. Five different LAMP primer sets were designed, and LAMP was performed. All primer sets targeting the 16S rRNA gene (BIP, FIP, B3, F3, LF, PF) were determined as positive in tcdA-positive, tcdB-postive ($A^+B^+$) and tcdA-negative, tcdB-negative ($A^-B^-$) Clostridium difficile strains. As the LAMP reaction took less than 80 min and did not require expensive machine such as thermocycler, it can be used as a rapid and simple detection method for foodborne pathogens.
Keywords
Clostridium difficile; LAMP; rapid detection; 16S rRNA gene;
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1 Noren T, Alriksson I, Andersson J, Akerlund T, Unemo M. 2011. Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard. J Clin Microbiol 49: 710-711.   DOI
2 Larson HE, Price AB, Honour P, Borriello SP. 1978. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet 1: 1063-1066.
3 Lee YJ, Choi MG, Lim CH, Jung WR, Cho HS, Sung HY, Nam KW, Chang JH, Cho YK, Park JM, Kim SW, Chung IS. 2010. Change of Clostridium difficile colitis during recent 10 years in Korea. Korean J Gastroenterol 55: 169-174.   과학기술학회마을   DOI   ScienceOn
4 Ricciardi R, Rothenberger DA, Mandoff RD, Baxter NN. 2007. Increasing prevalence and severity of Clostridium difficile colitis in hospitalized patients in the United States. Arch Surg 142: 624-631.   DOI   ScienceOn
5 Pepin J, Valiquette L, Cossette B. 2005. Mortality attributable to nosocomial Clostridium difficile-associated disease during an epidemic caused by hyper virulent strain in Quebec. CMAJ 173: 1037-1042.   DOI   ScienceOn
6 Tae CH, Jung SA, Song HJ, Kim SE, Choi HJ, Lee M, Hwang Y, Kim H, Lee K. 2009. The first case of antibiotic-associated colitis by Clostridium difficile PCR ribotype 027 in Korea. J Korean Med Sci 24: 520-524.   DOI   ScienceOn
7 O'Connor JR, Johnson S, Gerding DN. 2009. Clostridium difficile infection caused by the epidemic BI/NAP1/027 strain. Gastroenterology 136: 1913-1924.   DOI   ScienceOn
8 Rodriguez-Palacios A, Staempfli HR, Duffield T, Weese JS. 2007. Clostridium difficile in retail ground meat, Canada. Emerg Infect Dis 13: 485-487.   DOI
9 Songer JG. 2004. The emergence of Clostridium difficile as a pathogen of food animals. Anim Health Res Rev 5: 321-326.   DOI
10 McDonald LC. 2009. Surveillance for Disease and Sources of Infection: Initiatives at the Federal Level and International. Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention. Atlanta, GA, USA.
11 FDA. 2010. Draft Guidance for Industry and Food and Drug Administration Staff－Establishing the Performance Characteristics of In Vitro Diagnostic Devices for the Detection of Clostridium difficile. November 29, 2010. Food and Drug Administration, New Hampshire, MD, USA.
12 Gould LH, Limbago B. 2010. Clostridium difficile in food and domestic animals: a new foodborne pathogen? Clin Infec Dis 51: 577-582.   DOI
13 Lawley R. Clostridium difficile-a food safety hazard? http://www.foodsafetywatch.com/public/617.cfm
14 Patel JB, Leonard DG, Pan X, Musser JM, Berman RE, Nachamkin I. 2000. Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system. J Clin Microbiol 38: 246-251.
15 Braun V, Hundsberger T, Leukel P, Sauerborn M, von Eichel-Steiber C. 1996. Definition of the single integration site of the pathogenicity locus in Clostridium difficile. Gene 181: 29-38.   DOI
16 Harmsen D, Rothgänger J, Frosch M, Albert J. 2002. RIDOM: Ribosomal differentiation of medical microorganisms database. Nucleic Acids Res 30: 416-417.   DOI
17 Lemee L, Dhalluim A, Testelin S, Mattrat MA, Maillard K, Lemeland JF, Pons JL. 2004. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxinA), and tcdB (toxin B) genes for toxigenic culture of Clostridium difficile. J Clin Microbiol 42: 5710-5714.   DOI
18 Matamouros S, England P, Dupuy B. 2007. Clostridium difficile toxin expression is inhibited by the novel regulator TcdC. Mol Microbiol 64: 1274-1288.   DOI
19 Cohen SH, Tang YJ, Silva J Jr. 2000. Analysis of the pathogenicity locus in Clostridium difficile strains. J Infect Dis 181: 659-663.   DOI
20 Mori Y, Nagamine K, Tomita N, Notomi T. 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289: 150-154.   DOI   ScienceOn
21 Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. 2000. Loop-mediated isothermal amplification of DNA. Nucl Acid Res 28: e63.   DOI
22 Kato H, Yokoyama T, Kato H, Arakawa Y. 2005. Rapid and simple method for detecting the toxin B gene of Clostridium difficile in stool specimens by loop-mediated isothermal amplification. J Clin Microbiol 43: 6108-6112.   DOI