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Solubilization of Arabinogalactan by Extrusion from Portulaca oleracea L. and Its In Vitro Antioxidant Activity (마치현 아라비노갈락탄의 압출 수용화와 항산화 특성)

  • Choi, Ae-Jin;Jee, Ho-Kyun;Ko, Bo Sung;Kim, Yangha;Lee, Soo-Jeong;Kim, Chul-Jin;Cho, Yong-Jin;Kim, Chong-Tai
    • Food Engineering Progress
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    • v.13 no.3
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    • pp.169-175
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    • 2009
  • Water soluble polysaccharides (WSP) and arabinogalactan of Portulaca oleracea L. (POL) were increased after extrusion and commercial cellulase treatment. Arabinose and galactose content increased more about 1.5 times than those of raw POL, and rhamnose also increased about 2.6 times in WSP. High molecular weight fraction (I) of POL depending on extrusion condition including Ext I, Ext II and Ext III degraded into low molecular weight fraction (II) about 37, 29, and 26%, respectively, ranged from 67,000-69,000 Da of molecular weight. Especially, the molecular weight and composition of WSP with extruded, were increased from 9 to 13% in low molecular weight fraction, compared to those of raw POL. Solubilization and degradation of polysaccharides were a directly propotional to specific mechanical energy in POL extrusion. WSP obtained by extrusion at Ext I and Ext II were found to be effective antioxidants in different in vitro assays with regards to 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC). However, these results suggest that WSP obtained using extrusion and subsequent enzymatic treatment may be an effective method to produce arabinogalactan from POL and be used as a functional food ingredients.

A Study on the Morphological Analysis of Sperm (정자의 형태학적 특성 분석에 관한 연구)

  • Paick, Jae-Seung;Jeon, Seong-Soo;Kim, Soo-Woong;Yi, Won-Jin;Park, Kwang-Suk
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.2
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    • pp.153-165
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    • 1997
  • In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%${\sim}$-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%${\sim}$-5.7% and 6.0%${\sim}$-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.

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Weed Management Technology with Host Specific of Biological Control Agents (기주특이성 잡초 활성 미생물을 이용한 잡초방제 기술)

  • Hong, Yeon-Kyu;Lee, Bong-Choon;Song, Seok-Bo;Park, Sung-Tae;Kim, Jeong-Nam;Geon, Min-Goo;Kim, In-Seob
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.175-190
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    • 2006
  • The term mycoherbicide started in 1970, but its interest heightened due to increase costs of chemical herbicides. A classical biocontrol agent is expected to become a permanent part of its new environment and do no harm to it. Contemporary biological control agent(BCA) must be produced by artificial culture and could be applied like chemical herbicides over weeds. BCA is different from the classical approach in that it released through natural spread. To date 26 species of fungi are used as classical BCA against 26 species of weeds in seven countries. There are a number of examples of pathogens attacking non-target plants. But through risk assessments which include understanding the taxonomy, biology and ecology, the target and non-target species, it will be safe to introduce of exotic pathogens to control weeds. But pathogens have not been successfully used in practice. Many mycoherbicides show potential in laboratories, but are ineffective in the field and not consistent from year to year or field to field. There is also a lack of understanding humidity, dew formation and temperature and their effects on suppression of weeds by plant pathogens. Potential pathogen must be selected as a BCA. Previous studies suggest that these pathogens must (1) produce abundant and durable inoculum in artifical culture, (2) be genetically stable and weed specific and (3) kill weeds in control. A granular preparation of mycoherbicide into sodium alginate is lighter than liquids and less bulky than organic matter. Gel forms have also been used.

An Epidemiological Analysis of 28 Vivax Malaria Cases in Gimpo-si, Korea, 2020

  • Bahk, Young Yil;Cho, Shin-Hyeong;Kim, Kyoung-Nam;Shin, Eun-Hee;Jeon, Byoung-Hak;Kim, Jeong-Hyun;Park, Sookkyung;Kwon, Jeongran;Kan, Hyesu;Kim, Miyoung;Kim, Tong-Soo
    • Parasites, Hosts and Diseases
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    • v.59 no.5
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    • pp.507-512
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    • 2021
  • Since 1993, vivax malaria has been recognized as a public health burden in Korea. Despite of pan-governmental malaria-control efforts and the dramatic reduction in the burden of this disease over the last 10 years, vivax malaria has not been well controlled and has remained continuously endemic. We focused interviewed and examined the charts of 28 confirmed vivax malaria patients given malarial therapy for whom daily records were kept from Gimpo-si, Gyeonggi-do of Korea. Various epidemiological characteristics of vivax malaria, including the incubation period, medication used, and recurrence, and an evaluation of the parasitic characteristics from the focused interviews of patients from this region are described here. Most of the participants indicated the 3 most common symptoms of malaria (headache, chills and fever). Of the 28 cases, 2 experienced a second attack and there were 17 and 11 cases with short- and long-term incubation periods, respectively, yielding a short-term to long-term ratio of 1.5. Based on the parasitemia stages, most of the participants were tested at 5 to 7 days (11 cases) and 7 to 15 days (11 cases) after initial wave of asexual parasites. In conclusion, public health authorities should consider developing management measures to decrease the time lag for diagnosis and drafting unified and robust guidelines for drug use for malaria and drawing up unified and robust guidelines on the use of medication for malaria. It also suggests that routine monitoring, surveillance, and precise medical surveys in high-risk vivax malaria endemic areas are pivotal to controlling this persistent public disease and finally eliminating it from Korea.

A Study on the Application of BIPV for the Spread of Zero Energy Building (제로에너지 건축물 확산을 위한 건물 일체형 태양광 적용방안 연구)

  • Park, Seung-Joon;Jeon, Hyun-Woo;Lee, Seung-Joon;Oh, Choong-Hyun
    • Journal of Digital Convergence
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    • v.19 no.9
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    • pp.189-199
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    • 2021
  • In order to increase the self-reliance rate of new and renewable energy in order to respond to the mandatory domestic zero-energy buildings, the taller the building, the more limited the site area, and installing PV modules on the roof is not enough. Therefore, BIPV (Building integrated photovoltaic, hereinafter BIPV) is the industry receiving the most attention as a core energy source that can realize zero-energy buildings. Therefore, this study conducted a survey on the problems of the BIPV industry in a self-discussing method for experts with more than 10 years of experience of designers, builders, product manufacturers, and maintainers in order to suggest the right direction and revitalize the BIPV industry. Industrial problems of BIPV adjustment are drawn extention range of standard and certification for products, range improvement for current small condition of various kind productions, need to revise standards for capable of accomodating roof-type, color-module and louver-module, necessary of barrier in flow of foreign modules into korea through domestic certification mandatory, difficulty in obtaining BIPV information, request to prevent confusion among participants by exact guidelime about architectural application part of BIPV, and lack of the BIPV definition clearness, support policy, etc. Based on the improvements needed for the elements, giving change and competitiveness impacts aims to present and propose counter measures and direction.

An Experimental Study on the Influence of the Spread of Firebrand on Building Exterior Materials and Roofing Materials in Urban Areas (도심지 인접 산불의 불티 확산이 건축물 외장재와 지붕재에 미치는 영향에 관한 실험적 연구)

  • Min, Jeong-Ki
    • Journal of the Society of Disaster Information
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    • v.17 no.3
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    • pp.617-626
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    • 2021
  • Purpose: The purpose of this study is to evaluate the fire srpead risk of building exterior and roofing materials due to the firebrand of forest fire occurring in the urban areas. Method: In order to achieve this research purpose, by selecting building materials used for exterior and roofing materials of buildings, the time to ignition, total heat release, and heat release rate were investigated, and a forest fire firebrand system was established to the possibility of fire spread was confirmed. Result: As a result of the cone calorimeter test, the roofing material had a similar or faster ignition time due to radiant heat compared to the exterior material with the steel plate exposed to the outside, and showed a higher heat release rate and total heat release than the exterior material. Although it was affected by the flammable material, it was confirmed that it did not spread easily due to the limited amount of combustible material, and carbonization marks appeared inside. Conclusion: The cone calorimeter test method has been shown to be useful in understanding the combustion characteristics of building materials by radiant heat, but the fire spread due to a firebrand in a forest fire is directly affected by the flame due to the ignition of surrounding combustibles, so finding a direct correlation with the cone calorimeter method is difficult. It is judged that the roof material may be more vulnerable to the spread of fire due to the fire than the exterior material.

Development of Flood Rapid Defense System(FRDS) suitable for Southeast Asian Disaster (동남아시아 재난에 적합한 도심형 홍수임시차수시스템 개발)

  • Jung, In-Su;Oh, Eun-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.8-17
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    • 2018
  • A large urban region in Bangkok, Thailand is often inundated due to shallow water floods along the paved roads that have poor drainage facilities, and that can cause urban flooding. Existing methods, including using sand bags are not effective to prevent flooding in urban areas where the amount of sand is not sufficient. Thus, it is necessary to install artificial flood defense structures. However flooding and overflow defense equipment, which was developed in some advanced nations in Europe and in the USA, is highly expensive and complex construction methods are needed, therefore they are not suitable to be used in Southeast Asia. Thus, it is necessary to develop a flood rapid defense system(FRDS), which is inexpensive and simple to build, but is also highly functional. Thus, this study developed an FRDS that can be applied to Southeast Asia through the careful study of FRDS overviews, an analysis on the development trends in Korea and overseas, and the proposal of development needs and directions of the region. For the system developed, Korean Standards(KS) performance evaluations on leakage ratio deformation tests and impact resistance tests were conducted at the Outdoor Demonstration Test Center(Seosan) in the Korea Conformity Laboratories(KCL) and the system satisfied the standards of KS F 2639(leakage and deformation test) and KS F 2236(impact resistance test). The present study results can not only be applied to urban floods in Southeast Asian nations to cope with flood-related disasters, but also be utilized in flood prone regions and for major facilities in Korea. They can also induce scientific and pro-active responses from major local governments and facility management organizations in relation to urban floods.

Measurement of flash point for binary mixtures of Ethanol, 1-propanol, 2-propanol and 2,2,4-trimethylpentane (Ethanol, 1-propanol, 2-propanol 그리고 2,2,4-trimethylpentane 이성분 혼합계에 대한 인화점 측정)

  • Hwang, In Chan;In, Se Jin
    • Clean Technology
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    • v.25 no.2
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    • pp.140-146
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    • 2019
  • Flammable substances, such as organic solvents, are commonly used in laboratories and industrial processes. The flash point of flammable liquid mixtures is a very important parameter for characterizing the ignition and explosion hazards, and the flash points of mixtures of $C_2{\sim}C_3$ alcohols and 2,2,4-trimethylpentane were measured in the present study. The 2,2,4-trimethylpentane is an important component of gasoline and is frequently used in the petroleum industry as a solvent. Lower flash point data were measured for the binary systems {ethanol + 2,2,4-trimethylpentane}, {1-propanol + 2,2,4-trimethylpentane}, and {2-propanol + 2,2,4-trimethylpentane}. The flash point measurements were carried out according to the standard test method (ASTM D3278) using a Stanhope-Seta closed cup flash point tester. The measured flash points were compared with the predicted values calculated using Raoult's law and also following $G^E$ models: Wilson, Non-Random Two Liquid (NRTL) and UNIversal QUAsiChemical (UNIQUAC). These models were able to predict the experimental flash points for different compositions of {$C_2{\sim}C_3$ alcohols + 2,2,4-trimethylpentane} mixtures with minimal deviations. The average absolute deviation between the predicted and measured lower flash point was less than 1.28 K. A minimum flash point behaviour was observed in all of the systems as in the many observed cases for the hydrocarbon and alcohol mixtures.

Quantification of Chloride Diffusivity in Steady State Condition in Concrete with Fly Ash Considering Curing and Crack Effect (재령 및 균열효과를 고려한 플라이애시 콘크리트의 정상상태 염화물 확산 특성의 정량화)

  • Yoon, Yong-Sik;Cheon, Ju-Hyun;Kwon, Seung-Jun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.7 no.2
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    • pp.109-115
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    • 2019
  • In case of the cracks in concrete, the penetration of deterioration ions such as chloride ions in to cracks is accelerated. According to the penetration of chloride ions, structural and durability problems to RC(Reinforced Concrete) structures are caused. In this study, the accelerated chloride diffusion coefficient which is in steady state is evaluated for 2 year aged normal and high strength FA(Fly Ash) concrete, after a range of crack depths are induced up to 1.0 mm in 56 aged day. Considering crack effect by linear regression analysis, high strength concrete has slightly less increasing ratio of diffusion coefficient by crack than normal strength concrete, and diffusion coefficient increases non-linearly as crack width is increased. Also, In two types of concrete, crack effect decrease as the curing period increase. In the case of quantifying crack and curing effect by using exponential function form, the coefficients of determination are higher than those of linear regression analysis. Under steady state, it is thought that there is not a high correlation between the crack effect and the curing effect, and considering the two independent effects, it is believed that reasonable prediction equation for diffusion of concrete with crack can be proposed.

Evaluation of the Effectiveness of Surveillance on Improving the Detection of Healthcare Associated Infections (의료관련감염에서 감시 개선을 위한 평가)

  • Park, Chang-Eun
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.1
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    • pp.15-25
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    • 2019
  • The development of reliable and objective definitions as well as automated processes for the detection of health care-associated infections (HAIs) is crucial; however, transformation to an automated surveillance system remains a challenge. Early outbreak identification usually requires clinicians who can recognize abnormal events as well as ongoing disease surveillance to determine the baseline rate of cases. The system screens the laboratory information system (LIS) data daily to detect candidates for health care-associated bloodstream infection (HABSI) according to well-defined detection rules. The system detects and reserves professional autonomy by requiring further confirmation. In addition, web-based HABSI surveillance and classification systems use discrete data elements obtained from the LIS, and the LIS-provided data correlates strongly with the conventional infection-control personnel surveillance system. The system was timely, acceptable, useful, and sensitive according to the prevention guidelines. The surveillance system is useful because it can help health care professionals better understand when and where the transmission of a wide range of potential pathogens may be occurring in a hospital. A national plan is needed to strengthen the main structures in HAI prevention, Healthcare Associated Prevention and Control Committee (HAIPCC), sterilization service (SS), microbiology laboratories, and hand hygiene resources, considering their impact on HAI prevention.