Browse > Article

Solubilization of Arabinogalactan by Extrusion from Portulaca oleracea L. and Its In Vitro Antioxidant Activity  

Choi, Ae-Jin (Department of Herbal Crop Research, National Institute of Horiculture & Herbal Science, RDA)
Jee, Ho-Kyun (Bio-Nano Research Group, Korea Food Research Institute)
Ko, Bo Sung (Seoul Pharmal Laboratories, Inc.)
Kim, Yangha (Department of Food Nutrition, Ewha Womans University)
Lee, Soo-Jeong (Department of Food and Nutrition, Bucheon College)
Kim, Chul-Jin (Bio-Nano Research Group, Korea Food Research Institute)
Cho, Yong-Jin (Bio-Nano Research Group, Korea Food Research Institute)
Kim, Chong-Tai (Bio-Nano Research Group, Korea Food Research Institute)
Publication Information
Food Engineering Progress / v.13, no.3, 2009 , pp. 169-175 More about this Journal
Abstract
Water soluble polysaccharides (WSP) and arabinogalactan of Portulaca oleracea L. (POL) were increased after extrusion and commercial cellulase treatment. Arabinose and galactose content increased more about 1.5 times than those of raw POL, and rhamnose also increased about 2.6 times in WSP. High molecular weight fraction (I) of POL depending on extrusion condition including Ext I, Ext II and Ext III degraded into low molecular weight fraction (II) about 37, 29, and 26%, respectively, ranged from 67,000-69,000 Da of molecular weight. Especially, the molecular weight and composition of WSP with extruded, were increased from 9 to 13% in low molecular weight fraction, compared to those of raw POL. Solubilization and degradation of polysaccharides were a directly propotional to specific mechanical energy in POL extrusion. WSP obtained by extrusion at Ext I and Ext II were found to be effective antioxidants in different in vitro assays with regards to 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC). However, these results suggest that WSP obtained using extrusion and subsequent enzymatic treatment may be an effective method to produce arabinogalactan from POL and be used as a functional food ingredients.
Keywords
Portulaca oleracea L.; water soluble polysaccharides; arabinogalactan; extrusion; antioxidant activity;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Garti N, Slavin Y, Aserin A. 1999b. Competive adsorption in O/W emulsions stabilized by the new Portulaca oleracea hydrocolloidand nonionic emulsifiers. Food Hydrocolloid. 13: 139-144   DOI   ScienceOn
2 Amin ES, El-Deeb SM. 1977. Isolation of Portulaca oleracea(REGLA) mucilage and identification of its structure. Carbohyd.Res. 56: 123-128   DOI   ScienceOn
3 Chen J, Shi YP, Liu JY. 2003. Determination of noradrenaline anddopamine in Chinese herbal extracts from Portulaca oleracea L.by high-performance liquid chromatography. J. Chromatogr. A1003: 127-132   DOI   PUBMED   ScienceOn
4 Choi AJ, Kim CJ, Cho YJ, Cha JY, Hwang JK, Kim IH, Kim CT.2007. Characterization of polysaccharides obtained frompurslane (Portulaca olerace L.) using different solvents andenzymes. Food Sci. Biotechnol. 16: 928-934   과학기술학회마을   ScienceOn
5 D'Adamo P. 1996. Larch arabinogalactan. J. Naturopath Med. 6:33-37   ScienceOn
6 Malek F, Boskabady MH, Borushaki MT, Tohidi M. 2004. Bronchodilatoryeffect of Portulaca oleracea in airways of asthmaticpatients. J. Ethnopharamacol. 93: 57-62   DOI   ScienceOn
7 Parry O, Marks JA, Okwuasaba FK. 1993. The skeletal muscleralaxant action of Portulaca oleracea: role of potassium ions. J.Ethanopharmacol. 40: 187-194   DOI   ScienceOn
8 Lim JP, Suh ES. 2000. Hepatoprotective, diuretic and anti-inflammatoryactivities of the extract from Portulaca oleracea Linne.Korean J. Med. Crop Sci. 8: 189-193   ScienceOn
9 Perez S, Mazeau K, Penhoat CH. 2000. The three-dimensionalstructures of the pectic polysaccharides. Plant Physiol. Biochem.38: 37-55   DOI   ScienceOn
10 Jee HK, Cho YJ, Kim CT, Jang YS, Kim CJ. 2006. Increase ofsolubility ginseng radix by extrusion cooking. Korean J. FoodSci. Technol. 38: 361-368   ScienceOn
11 Dubois M, Gilles KA, Hamilton JK, Rebers, PA, Smith F. 1956.Colorimetric method for determination of sugars and relatedsubstances. Anal. Chem. 28: 350-356   DOI
12 Liu L, Howe P, Zhou YF, Xu ZQ, Hocart C, Zhang R. 2000.Fatty acids and β-carotene in Australian pulsane (Portulaca oleracea)varieties. J. Chromatogr. A. 893: 207-213   DOI   ScienceOn
13 Sakai N, Inada K, Okamoto M, Shizuri Y, Fukuyama Y. 1996.Portuloside A, a monoperpene glucoside, from Portulaca oleracea.Phytochemistry 42: 1625-1628   DOI   ScienceOn
14 Chan K, Islam MW, Kamil M, Radhakrishman MNMZ, HabibullahM, Attas A. 2000. The analgesic and anti-inflammatoryeffects of Portulaca oleracea L. subsp. sativa (Haw.) Celak. J.Ethanopharmacol. 73: 445-451   DOI   ScienceOn
15 Bae JH. 1999. Effect of Portulaca oleracea extract on removingnicotine component of tabacco. J. Korean Soc. Food Sci. Nutr.28: 670-612   ScienceOn
16 Yu KW, Hwang JH. 2001. Characterization of bone marrow cellproliferating arabinogalactan through peyer's patch cells fromrhizomes of atractylodes lancea DC. J. Food Sci. Nutr. 6: 180-186   ScienceOn
17 Theander O, Aman P, Westerlund E, Anderson R, Pettersson D.1995. Total dietary fiber as neutral sugar residues, uronic acidresidue, and Klason lignin (The Uppsala method): Collaborativestudy. J. AOAC Int. 78: 1030-1044   PUBMED   ScienceOn
18 Choi EM, Kim AJ, Hwang JK. 2005. Enhanced immune cellfuctions and cytokine production after in vitro stimulation witharabinoxylans fraction from rice bran. Food Sci. Biotechnol. 14:479-486   과학기술학회마을   ScienceOn
19 Habtemarian S, Harvey AL, Waterman PG. 1993. the musclerelaxant properties of Portulaca oleracea are associated withhigh concentrations of potassium ions. J. Ethanopharmacol. 40:195-200   DOI   ScienceOn
20 Na K, Yun JM, Choi MJ, Hwang JK. 2004. Extraction of pecticpolysaccharides from extruded ginseng fiber. Food Sci. Biotechnol.13: 486-489   ScienceOn
21 Eriksson I, Andersson R, Aman P. 1997. Extraction of pecticsubstances from dehulled rapeseed. Carbohydr. Res. 301: 177-185   DOI   ScienceOn
22 Seo Y, Shin J, Cha HJ, Kim YA, Ahn JW, Lee BJ, Lee DS. 2003.A new monoterpene glucoside from Portulaca oleracea. Bull.Korean Chem. Soc. 24: 1475-1477   DOI   ScienceOn
23 Mohamed A, Hussein A. 1994. Chemical composition of purslane(Portulaca oleracea). Plant Food Hum. Nutr. 45: 1-9   DOI   ScienceOn
24 Van den Berg R, Haenen GRMM, Van den Berg H. 1999. Applicabilityof an improved Trolox equivalent antioxidant capacityassay for evaluation of antioxidant capacity measurements ofmixtures. Food Chem. 66: 511-517   DOI   ScienceOn
25 Sridhar R, Lakshminarayana G. 1993. Lipid classes, fatty acids,and tocopherols of leaves of six edible plant species. J. Agric.Food Chem. 41: 61-63   DOI
26 Lee TB. 1999. Illustrated flora of Korea. Hyangmunsa, Seoul,Korea, p. 324
27 Sridhar R, Lakshminarayana G. 1993. Lipid classes, fatty acids,and tocopherols of leaves of six edible plant species. J. Agric.Food Chem. 41: 61-63   DOI
28 Garti N, Aserin A, Slavin Y. 1999a. Portulaca oleracea gum andcasein interactions and emulsion stability. Food Hydrocolloid.13: 127-138   DOI   ScienceOn
29 Lee MS, Kim CT, Kim CJ, Cho YJ, Kim YH. 2006. Effects ofPortulaca oleracea L. extract on lipolysis and hormone sensitivelipase (HSL) gene expression in 3T3-L1 adipocytes. Korean J.Nutr. 39: 742-747   ScienceOn
30 Omara-Alwala T, Mebrahtu T, Prior DE, Ezekwe M. 1991.Omega-three fatty acids in pulsane (Portulaca oleracea) tissues.JAOCS 68: 198-199   DOI
31 Kelly GS. 1999. Larch arabinogalactan: Clinical relevance of anovel immune-enhancing polysaccharide. Alter. Med. Rev. 4:96-103   PUBMED   ScienceOn