• Title/Summary/Keyword: L-A-S

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The Evidence for Pepsin-Catalyzed Transpeptidation (펩신촉매에 의한 Transpeptide의 생성)

  • 조용권
    • Journal of Life Science
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    • v.8 no.4
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    • pp.410-415
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    • 1998
  • Procine pepsin hydrolysis of hexapeptide L-S-pNF-Nle-A-OMe in the presence of dipeptide L-L generates a new peak on HPLC analysis of reaction mixtures that is not seen when enzyme is incubated with either peptide alone. The peaks can be detected spectroscopically at either 214 or 254 nm, the latter consistent with a new peptide containing the p-nitro-F residue. The data suggest acyl transpeptidation between E(L-S-pNF) and L-L to form L-S-pNF-L-L. Consistent with this inference are (1) the ability of L-L-NH$_{2}$ and inability of Boc-L-L to undergo a similar transpeptidation reaction, and (2) the data from electrospray mass spectrum. This synthesis requires that Nle-A-L-OMe be released before L-S-pNF, an order opposite to that proposed on the basis of product inhibition kinetics. Consistent with this inference are reciprocal solvent isotope effects ; normal isotope effects of 1.736$\pm$0.121 on the formation of Nle-A-L-OMe and 2.281$\pm$0.184 in the formation of L-S-pNF, coupled to an inverse isotope effects of 0.576$\pm$0.045 on the formation of L-S-pNF-L-L. Because transpeptidation precedes faster in D$_{2}$O, the isotopically-sensitive step must occur after release of Nle-A-L-OMe. Isotopically-enhanced transpeptidation is consistent with the Uni-Bi iso memchanism postulated on the basis of an isotope effects on Vmax but not on Vmax/Km$^{1)}$ and confirmed by isotope effects on the onset of inhibition by pepstatin$^{2)}$.

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AN ANALOGUE OF THE HILTON-MILNER THEOREM FOR WEAK COMPOSITIONS

  • Ku, Cheng Yeaw;Wong, Kok Bin
    • Bulletin of the Korean Mathematical Society
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    • v.52 no.3
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    • pp.1007-1025
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    • 2015
  • Let $\mathbb{N}_0$ be the set of non-negative integers, and let P(n, l) denote the set of all weak compositions of n with l parts, i.e., $P(n,l)=\{(x_1,x_2,{\cdots},x_l){\in}\mathbb{N}^l_0\;:\;x_1+x_2+{\cdots}+x_l=n\}$. For any element $u=(u_1,u_2,{\cdots},u_l){\in}P(n,l)$, denote its ith-coordinate by u(i), i.e., $u(i)=u_i$. A family $A{\subseteq}P(n,l)$ is said to be t-intersecting if ${\mid}\{i:u(i)=v(i)\}{\mid}{\geq}t$ for all $u,v{\epsilon}A$. A family $A{\subseteq}P(n,l)$ is said to be trivially t-intersecting if there is a t-set T of $[l]=\{1,2,{\cdots},l\}$ and elements $y_s{\in}\mathbb{N}_0(s{\in}T)$ such that $A=\{u{\in}P(n,l):u(j)=yj\;for\;all\;j{\in}T\}$. We prove that given any positive integers l, t with $l{\geq}2t+3$, there exists a constant $n_0(l,t)$ depending only on l and t, such that for all $n{\geq}n_0(l,t)$, if $A{\subseteq}P(n,l)$ is non-trivially t-intersecting, then $${\mid}A{\mid}{\leq}(^{n+l-t-l}_{l-t-1})-(^{n-1}_{l-t-1})+t$$. Moreover, equality holds if and only if there is a t-set T of [l] such that $$A=\bigcup_{s{\in}[l]{\backslash}T}\;A_s{\cup}\{q_i:i{\in}T\}$$, where $$A_s=\{u{\in}P(n,l):u(j)=0\;for\;all\;j{\in}T\;and\;u(s)=0\}$$ and $$q_i{\in}P(n,l)\;with\;q_i(j)=0\;fo\;all\;j{\in}[l]{\backslash}\{i\}\;and\;q_i(i)=n$$.

ON f-DERIVATIONS FROM SEMILATTICES TO LATTICES

  • Yon, Yong Ho;Kim, Kyung Ho
    • Communications of the Korean Mathematical Society
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    • v.29 no.1
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    • pp.27-36
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    • 2014
  • In this paper, we introduce the notion of f-derivations from a semilattice S to a lattice L, as a generalization of derivation and f-derivation of lattices. Also, we define the simple f-derivation from S to L, and research the properties of them and the conditions for a lattice L to be distributive. Finally, we prove that a distributive lattice L is isomorphic to the class $SD_f(S,L)$ of all simple f-derivations on S to L for every ${\wedge}$-homomorphism $f:S{\rightarrow}L$ such that $f(x_0){\vee}f(y_0)=1$ for some $x_0,y_0{\in}S$, in particular, $$L{\simeq_-}=SD_f(S,L)$$ for every ${\wedge}$-homomorphism $f:S{\rightarrow}L$ such that $f(x_0)=1$ for some $x_0{\in}S$.

Pro-apoptotic Effects of S100A8 and S100A9 on human FIP1L1-PDGFRα+ Eosinophilic Leukemia Cells

  • Lee, Ji-Sook
    • Biomedical Science Letters
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    • v.27 no.2
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    • pp.95-98
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    • 2021
  • The S100 family proteins act as inducers of cancer cell apoptosis and inflammatory mediators. This study examined the pro-apoptotic mechanism caused by S100A8 and S100A9 in human FIP1L1-PDGFRα-positive eosinophilic leukemia cells. S100A8 and S100A9 elicited the death of EoL-1 cells in a time and dose-dependent manner. The activation of PDGFRα was suppressed by a decrease in PDGFRα after treatment with S100A8 and S100A9. Cycloheximide, a translation inhibitor, suppressed PDGFRα expression from 1 h to 5 h, and a co-treatment with S100A8 and S100A9 boosted the decrease in expression. The phosphorylation and expression of STAT5 decreased after treatment with S100A8 and S100A9 in EoL-1 and imatinib-resistant (EoL-1-IR) cells. S100A8 and S100A9 induced the chemotaxis of EoL-1 cells but did not affect the chemoattraction of EoL-1-IR. These findings indicate the cell death mechanism due to S100 family proteins and the development of leukemia therapy using S100A8 and S100A9.

A Study on implementation of Simplify Chua's Circuit without L component (L성분이 없는 간략화 Chua 회로 구현에 관한 연구)

  • Shon, Youngwoo;Bae, Youngchul
    • The Journal of the Korea institute of electronic communication sciences
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    • v.5 no.1
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    • pp.17-22
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    • 2010
  • Generally, there are Chua's Circuit, Lorenz Circuit and Duffing circuit in the chaos circuit. Among these chaos circuits, Chua's circuit is well known to make the electronic parts easily. Chua's circuit is the constitute of the linearelements. These are constitute of Resistor component(R), inductor component(L), capacitor(C), and nonlinear element which is constitute of nonlinear resistor. However, L element have a difficult problem to implement real hardware by using commercial parts. Due to this, it has a saturation characteristic. In this paper, we analyzed the simplified Chua's circuit which is replace L to C by PSPICE program. Because L element has a difficult problem to make a real hardware, L has a saturation characteristic and we also confirm this analysis as the result.

A UNIFORM LAW OF LARGE MUNBERS FOR PRODUCT RANDOM MEASURES

  • Kil, Byung-Mun;Kwon, Joong-Sung
    • Bulletin of the Korean Mathematical Society
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    • v.32 no.2
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    • pp.221-231
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    • 1995
  • Let $Z_1, Z_2, \ldots, Z_l$ be random set functions or intergrals. Then it is possible to discuss their products. In the case of random integrals, $Z_i$ is a random set function indexed y a family, $G_i$ say, of real valued functions g on $S_i$ for which the integrals $Z_i(g) = \smallint gdZ_i$ are well defined. If $g_i = \in g_i (i = 1, 2, \ldots, l) and g_1 \otimes \cdots \otimes g_l$ denotes the tensor product $g(s) = g_1(s_1)g_2(s_2) \cdots g_l(s_l) for s = (s_1, s_2, \ldots, s_l) and s_i \in S_i$, then we can defined $Z(g) = (Z_1 \times Z_2 \times \cdots \times Z_l)(g) = Z_1(g_1)Z_2(g_2) \cdots Z_l(g_l)$.

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Analysis of the Reaction Steps in the Bioconversion of D,L-ATC to L-Cysteine

  • Ryu, Ok-Hee;Shin, Chul-Soo
    • Journal of Microbiology and Biotechnology
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    • v.1 no.1
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    • pp.50-53
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    • 1991
  • The reaction steps involved in the bioconversion of a chemically synthesized precursor, $D,L-2-amino-{\Delta}^2-thiazoline-4-carboxylic$ acid (D,L-ATC), to L-cysteine and the properties of the involved enzymes were investigated. It was found that the conversion consisted of two steps, i. e., D,L-ATC to S-carbamyl-L-cysteine (S-C-L-cysteine) and S-C-L-cysteine to L-cysteine, and the S-C-L-cysteine was an intermediate between them. While the enzymes involved in the reactions were induced by the addition of D,L-ATC as an inducer, S-C-L-cysteine induced only the enzyme involved in the latter step. The conversion of S-C-L-cysteine to L-cysteine could be also carried out in the presence of hydroxylamine and its rate was much faster than that by the corresponding enzyme. On the other hand, L-cysteine (or L-cystine) was decomposed to evolve $H_2S$ by the enzyme considered to be a kind of desulfhydrase. However, hydroxylamine was a perfect inhibitor for this enzyme.

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Antipathogenic Activity of Lactobacillus plantarum Isolated from Pickled Mulberry Leaf (뽕잎 장아찌로부터 분리된 Lactobacillus plantarum 균주의 유해균 증식 억제 활성)

  • Park, Eun-Hee;Kim, Myoung-Dong
    • Microbiology and Biotechnology Letters
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    • v.44 no.2
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    • pp.163-170
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    • 2016
  • Strains of lactic acid bacteria were isolated from a variety of fermented foods collected in Korea. The strain L2167 showed a strong antipathogenic activity against Bacillus cereus, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus, and Staphylococcus epidermidis. L2167 was identified as Lactobacillus plantarum by sequence analysis of its 16S rRNA gene. Scanning electron microscopy revealed rough and wrinkled morphology of B. cereus, L. monocytogenes, S. Typhimurium, S. aureus, and S. epidermidis cell membranes after treatment with a crude cell extract of L. plantarum L2167, indicating that Lactobacillus plantarum L2167 might destroy the cell membrane of pathogenic bacteria. The optimal temperature and initial medium pH for Lactobacillus plantarum L2167 growth were 35℃ and 5.5, respectively. Lactobacillus plantarum L2167 was more sensitive to NaCl than Lactobacillus plantarum KCTC21004, used as a control strain. Lactobacillus plantarum L2167 is expected to be developed as a prominent starter strain for efficient inhibition of growth of pathogens.

Identification and Tolerance-Test to Digestive Fluids of Lactobacilli Isolated from Korean Liquid Yogurts (국내 액상발효유에서 분리한 유산균의 동정 및 소화관액 내성조사)

  • So, Myeong-Hwan
    • Korean Journal of Food Science and Technology
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    • v.17 no.3
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    • pp.192-196
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    • 1985
  • Eight strains of Lactobacilli(a, b, b', c, d, e, f and g) were isolated from seven Korean liquid-yogurts(A, B, C, D, E, F and G), and identification and tolerance-test to digestive fluids were carried out. Isolate a from yogurt A and isolate a from yogurt E were identified as L. casei, isolate b from yogurt B as L. acidophilus, isolate d from yogurt D as L. bulgaricus, isolate f from yogurt F as L. helveticus, and isolate b' from yogurt B, isolate c from yogurt C and isolate g from yogurt G as L. jugurti, respectively. Isolate f(L. helveticus) and c(L. jugurti) showed high tolerance to artificial gastric juice but didn't to bile acid. Isolate b(L. acidophilus), a(L. casei), and e(L. casei) showed high tolerance to both artificial gastric juice and bile acid, but isolate d(L. bulgaricus), b'(L. jurgurti) and g(L. jugurti) did not.

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SUMMING AND DOMINATED OPERATORS ON A CARTESIAN PRODUCT OF c0 (𝓧) SPACES

  • Badea, Gabriela;Popa, Dumitru
    • Journal of the Korean Mathematical Society
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    • v.54 no.3
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    • pp.967-986
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    • 2017
  • We give the necessary condition for an operator defined on a cartesian product of $c_0(\mathcal{X})$ spaces to be summing or dominated and we show that for the multiplication operators this condition is also sufficient. By using these results, we show that ${\Pi}_s(c_0,{\ldots},c_0;c_0)$ contains a copy of $l_s(l^m_2{\mid}m{\in}\mathbb{N})$ for s > 2 or a copy of $1_s(l^m_1{\mid}{\in}\mathbb{N})$, for any $l{\leq}S$ < ${\infty}$. Also ${\Delta}_{s_1,{\ldots},s_n}(c_0,{\ldots},c_0;c_0)$ contains a copy of $l_{{\upsilon}_n(s_1,{\ldots},s_n)}$ if ${\upsilon}_n(s_1,{\ldots},s_n){\leq}2$ or a copy of $l_{{\upsilon}_n(s_1,{\ldots},s_n)}(l^m_2{\mid}m{\in}\mathbb{N})$ if 2 < ${\upsilon}_n(s_1,{\ldots},s_n)$, where ${\frac{1}{{\upsilon}_n(s_1,{\ldots},s_n})}={\frac{1}{s_1}}+{\cdots}+{\frac{1}{s_n}}$. We find also the necessary and sufficient conditions for bilinear operators induced by some method of summability to be 1-summing or 2-dominated.