• 한국양식학회지
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• 제20권2호
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• pp.121-126
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• 2007

밀폐순환 유수식 산소소비 측정장치를 이용하여 해수 및 담수에서 사육한 감성돔, Acanthopagrus schlegeli의 개체수에 따른 산소소비 경향을 조사하였다. 해수 및 담수순화된 평균체중 36.4 g의 감성돔을 각각 1마리와 3마리 그룹으로 나누어 수온(15, 20, $25^{\circ}C$), 광주기(12L:12D)를 달리하여 실험하였다. 모든 실험조건에서 감성돔은 명기동안에 산소소비량이 많고, 암기동안에는 적은 뚜렷한 리듬을 보였으며, 수온상승과 비례하여 산소소비량이 증가하였다. 감성돔의 호흡수는 담수돔 보다 해수돔이 많았으며, 수온상승과 비례하여 증가하였다. 수온 15, 20, $25^{\circ}C$에서 개체수가 1마리일 때 해수돔의 산소소비량은 각각 140.0, 174.8, 282.3 mg $O^2{\cdot}kg^{-1}{\cdot}h^{-1}$, 담수돔은 177.5, 307.8, 413.1 mg $O^2{\cdot}kg^{-1}{\cdot}h^{-1}$로 해수돔에 비해 담수돔의 산소소비량이 많았다. 그러나 개체수가 3마리일 때는 해수돔 각각 200.5, 274.7, 339.1 mg $O^2{\cdot}kg^{-1}{\cdot}h^{-1}$, 담수돔 177.5, 307.8, 413.1 mg $O^2{\cdot}kg^{-1}{\cdot}h^{-1}$로 담수돔 보다 해수돔의 산소소비량이 많았다.

• 생태와환경
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• 제40권2호
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• pp.303-317
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• 2007

소규모 담수생태계의 어류군집은 하천의 직, 간접적인 변화에 의해 크게 영향을 받는다. 하천의 변화를 유발하는 요소 중 하나인 도시화에 따른 어류상의 변화를 파악하기 위하여 부산시 일대의 어류상 조사를 실시하였다. 본 조사는 부산시 일대의 하천을 동부, 중부, 서부의 3지역으로 구분하여 2001년부터 2004년에 걸쳐 모두 55개 지점에서 실시하였다. 조사기간 동안 총 19과 46종 3,206개체의 어류가 채집되었고, 우점종은 Rhynchocypris oxycephalus, 아우점종은 Zacco temminckii였다. 수계별로 채집된 어류 중 일차 담수어를 대상으로 집괴 분석을 실시한 결과 전반적으로 동부, 중부, 서부지역이 구분되는 양상을 보였으며, Z. temminckii, R. oxycephalus가 상류를, Carassius cuvieri, Acheilognathus rhombeus, Hemiculter eigenmanni, Micropterus salmoides가 중, 하류를 대표하는 어종이었다. 문헌조사와 본 조사의 자료를 집괴 분석 한 결과 도시화가 진행되었던 동부 기장지역이 부산시 중부지역과 유사한 패턴을 보였다. 향후 하천복원과 동시에 이루어질 어류 복원시 각 하천의 특성과 어류의 지사적 분포 분석을 통하여 적합한 종 선별 및 서식처 복원이 이루어져야 할 것이며, 또한 지속적인 관리를 위한 모니터링이 요구된다.

• 한국동물위생학회지
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• 제21권4호
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• pp.431-437
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• 1998

Argulus spp, a small group of parasitic curstaceans called Branchiura, is particularly important in freshwater acquaculture. We investigated the infection of Argulus to several different species of fishes and therapy effect of masoten. Argulus japonicus was collected from Okjeong lake, Chonbuk province and experlmen-tally infected to freshwater fishes from July to August 1998. The results were as follows ; 1. The shape of a Agulus is an oval and dorsoventrally flattened, with first maxillae and second maxillae, and four pairs of legs. 2. The size of the adult stage of A japonicus was 3.2-5.0$\times$2.3-2.9mm in female and 1.5-3.1$\times$1.6-2.9mm in male. 3. In the trial of experimental infection with A japonicus to non-scaled fishes, Parasilurus asotus and Ictalurus punctalus, were infected and some of them died after severe infection. However, A japonica was not infected at all. 4. Masoten treatment for A japonicus was effective at 0.2 ppm once a day for 3 days and parasites on the surface of fishes disappeared after five days.

• 한국양식학회지
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• 제12권2호
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• pp.145-153
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• 1999

This study was conducted to determine the protein requirement of ayu (Plecoglossus altivelis). Two replicate groups of fish initially averaging 6.6 g were fed the five isocaloric diets containing different protein level from 29% to 57% in a flow-through freshwater system for 25 days. White fish meal was used as a sole protein source. Weight gain and feed efficiency of fish increased significantly with dietary protein level up to 43% (P＜0.05) with no additional response above this level. Protein and lipid retention, moisture, protein and lipid contents of body were not affected by dietary protein levels (P>0.05). Daily protein intake increased significantly with dietary protein level, whereas protein efficiency ratio of fish fed the 57% dietary protein decreased (P<0.05). The data obtained in this study indicate that a 43% dietary protein level could be recommended for the optimum growth of ayu.

• 한국수산과학회지
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• 제44권4호
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• pp.352-358
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• 2011

The presence of ISKNV-like viruses in various freshwater ornamental fish species imported from Asia was confirmed by polymerase chain reaction(PCR) amplification of the ATPase(adenosine triphosphatase) gene. Interestingly, molecular analyses of the Open Reading Frame 25(ORF25) region of these isolates based on the ISKNV(Infectious spleen and kidney necrosis virus) genome revealed the presence of various repetitive sequences. ORF25 repeat sequence length had no effect on cumulative mortality of rock bream Oplegnathus fasciatus challenged with tissue homogenates of infected pearl gourami, Trichogaster leeri; silver gourami, Trichogaster microlepis; blue gourami, or Trichogaster trichopterus. All isolates induce cumulative mortalities after 12 days of infection, confirming that ORF25 polymorphism did not affect the pathogenicity of ornamental fish megalocytiviruses that cross infect rock bream, a seawater fish. Also, no statistically significant differences in spleen index or viral copy number in infected tissues was detected between isolates with varying ORF25 repeat sequence lengths. However, further studies are necessary to fully characterize the functional characteristics of these polymorphisms in megalocytivirus disease in ornamental fishes.

• 한국수산과학회지
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• 제33권2호
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• pp.119-123
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• 2000

은연어가 운반과정 중에 어느 정도 스트레스를 받는지 조사하기 위하여 어류를 각각 담수와 염수 ($5{\%{\circ}}$)를 채운 수조차에 싫고 10 시간 운반하였다. 운반하기 전후에 혈청 중의 1차 스트레스 지표 물질인 코티졸을 비롯하여 2차 지표물질인 GLC, LAC, TG, CHOL, 전해질 ($Na^+, Cl^-$), 효소 (ALT, AST, LDH) 활성을 측정하여 두 운반구 간의 차이를 비교하였다. 그 결과, 운반작업은 은연어에게 상당한 스트레스를 주는 것으로 확인되었으나, 운반방법에 따라서는 스트레스 반응에 차이가 있었다. 즉, 담수운반구에 비해 염수운반구는 코티졸, GLC, LAC, TG, CHOL, AST 및 ALT가 유의적인 차이를 보이며 낮은 수준이었다. 이것으로 은연어의 운반시에는 담수보다 염수를 사용하는 것이 스트레스를 줄일 수 있다는 것을 알 수 있었다.

• 한국수산과학회지
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• 제36권3호
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• pp.263-269
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• 2003

Morphological development of eggs, larvae and juvenile of the sunrise sculpin, Pseudoblennius cottoides were studed in the field and laboratory at the coastal Dolsan-do, Yeosu-shi from October, 2000 to April, 2001 Egg mass of Pseudoblennius cottoides in peribranchial cavity of Halocynthia hilgendorfi, were observed during late fall to winter in the study area. Fertiliged eggs were spherical in shape, demersal, adhesive, transparent and greenwish yellow color, measuring 1.84 mm (1.83-1.87 mm) in diameter. There were numerous and 17 (15-20) various-sized oil globules accounted in the yolk. Granular materials formed a mass in the yolk. Fertiliged eggs hatched at 301 hr 20 min after morula stage. Newly hatched larvae 6.31 mm (6.24-6.37 mm) in total length (TL), had a large yolk. At 3 days after hatching, the larvae, 6.77 mm (6.69-7.14 mm) in TL came out through the excurrent siphon of Halocynthia hilgendorfi. At 13 days after hatching, the larvae 12.59 mm (12.42-12.63 mm) in TL transformed to postlarval stage. At 32 days after hatching, postlarvae of TL 19.18 mm (19.01-19.46 mm) have reached the juvenile stage.

• 생태와환경
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• 제49권2호
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• pp.130-141
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• 2016

This study was conducted to elucidate the structure of river ecosystem in the watershed of the Seom River and Hoengseong Reservoir located in Hoengseong of Kangwon Province from February to October 2007. Topics of the survey were mainly rainfall, discharge, water quality in abiotic factors and attached algae, benthic macroinvertebrates, fish, birds and mammals of flora and fauna in a biotic factors, respectively. Specifically, the Seom River could be seen as a typical flow rate of the stream is controlled to the effect of the dam. Basic water qualities were great to seasonal effects, it was relatively clean. Diatom Achnanthes, Cymbella, Gomphonema, and Navicula were distributed predominantly in the periphytic algae. Benthic macroinvertebrates were mostly aquatic insects and freshwater shellfish, the aquatic insects were abundant Ephemeroptera and Trichoptera. Freshwater fish was the dominant Zacco platypus, fish species varied toward the downstream. Birds were mainly observed in four species, and species Egretta garzetta, which was distributed in a wide area of the Seom River. In mammals, Lutra lutra of Mustelidae was identified that the number of inhabit widely. In aspects of the ecological trophic level, the Seom River was maintained at a relatively stable state in the producer and the consumer relationship. The results of this study will expected to be utilized as a useful data for understanding the structure and function of the lotic and lentic ecosystems.

• 생태와환경
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• 제44권4호
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• pp.364-372
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• 2011

The pale chub (Zacco platypus) is generally found in Asian countries, such as Korea, Japan, and China. Nevertheless, very little information exists about the genes involved in the metabolism of xenobiotics in this species. This species is useful in monitoring the environmental impact on various pollutants in freshwater as a sentinel fish species. We cloned the full-length cDNA sequence of xenobiotic metabolizing cytochrome P450 1A (CYP1A) gene from Z. platypus and characterized it. Tissue distribution and timedependent induction of CYP1A were studied by real-time RT-PCR. Induction pattern of CYP1A was studied by exposing the fish to an arylhydrocarbon receptor agonist, ${\beta}$-naphthoflavone (BNF). The liver showed the highest level of expression in basal state as well as BNF- treated fish. However, appreciable levels of expression were also recorded in Gill and kidney and the least level of expression was observed in the eye. The results of the time-course study revealed an induction in the liver, brain, and gills after 6 h and 12 h in most of the tissues. This study provides an insight into the xenobiotics metabolizing system of Z. platypus and offers baseline information for further research related to biomarker, stress, and adaptive response of this ecologically important fish species in the freshwater environment.

• Parasites, Hosts and Diseases
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• 제51권1호
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• pp.107-114
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• 2013

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, and Centrocestus formosanus) were detected. O. viverrini was detected in 35 fish (14.5%), and their density was 252 per infected fish (Luang Prabang, 88 metacercariae in 5 fish; Khammouane, 187 in 6 fish; Saravane, 303 in 24 fish). H. taichui was found in 102 fish (42.1%), and their density was 485 per infected fish (Luang Prabang, 260 metacercariae in 38 fish; Khammouane, 1,084 in 23 fish; Saravane, 359 in 41 fish). H. yokogawai was detected in 92 fish (38.0%), and their density was 222 per infected fish (Luang Prabang, 362 metacercariae in 17 fish; Khammouane, 126 in 20 fish; Saravane, 214 in 55 fish). Metacercariae of C. formosanus were found in 8 fish (3.3%), and their density was 3 per infected fish. In the present study, it has been confirmed that FBT metacercariae, in particular, H. taichui, H. yokogawai, and O. viverrini, are highly prevalent in fish from Luang Prabang, Khammouane, and Saravane Province, Lao PDR.