Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Fishborne Trematode Metacercariae in Luang Prabang, Khammouane, and Saravane Province, Lao PDR

  • Rim, Han-Jong (Department of Parasitology, Korea University College of Medicine) ;
  • Sohn, Woon-Mok (Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Yong, Tai-Soon (Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Eom, Keeseon S. (Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Min, Duk-Young (Department of Microbiology and Immunology, Eulji University College of Medicine) ;
  • Lee, Soon-Hyung (Korea Association of Health Promotion) ;
  • Hoang, Eui-Hyug (Korea Association of Health Promotion) ;
  • Phommasack, Bounlay (Department of Hygiene and Prevention, Ministry of Public Health) ;
  • Insisiengmay, Sithat (Department of Hygiene and Prevention, Ministry of Public Health)
  • Received : 2012.05.06
  • Accepted : 2012.07.02
  • Published : 2013.03.15
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Abstract

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, and Centrocestus formosanus) were detected. O. viverrini was detected in 35 fish (14.5%), and their density was 252 per infected fish (Luang Prabang, 88 metacercariae in 5 fish; Khammouane, 187 in 6 fish; Saravane, 303 in 24 fish). H. taichui was found in 102 fish (42.1%), and their density was 485 per infected fish (Luang Prabang, 260 metacercariae in 38 fish; Khammouane, 1,084 in 23 fish; Saravane, 359 in 41 fish). H. yokogawai was detected in 92 fish (38.0%), and their density was 222 per infected fish (Luang Prabang, 362 metacercariae in 17 fish; Khammouane, 126 in 20 fish; Saravane, 214 in 55 fish). Metacercariae of C. formosanus were found in 8 fish (3.3%), and their density was 3 per infected fish. In the present study, it has been confirmed that FBT metacercariae, in particular, H. taichui, H. yokogawai, and O. viverrini, are highly prevalent in fish from Luang Prabang, Khammouane, and Saravane Province, Lao PDR.

Keywords

References

  1. WHO. Food-borne trematode infections in Asia. Report Joint WHO/FAO Workshop, Hanoi, Vietnam. 2002.
  2. Chai JY, Murrell KD, Lymbry A. Fishborne parasitic zoonoses: status and issues. Int J Parasitol 2005; 35: 1233-1254. https://doi.org/10.1016/j.ijpara.2005.07.013
  3. Neveu R. Laos, the horizons travel guide series. Extra Image, Bangkok, Thailand. 1999.
  4. Lao National Geographic Department. Lao geographic atlas. Vientiane, Lao PDR. 2000.
  5. Rim HJ, Chai JY, Min DY, Cho SY, Eom KS, Hong SJ, Sohn WM, Yong TS, Deodato G, Standgaard H, Phommasack B, Yun CY, Hoang EH. Prevalence of intestinal parasite infections on a national scale among primary schoolchildren in Laos. Parasitol Res 2003; 91: 267-272. https://doi.org/10.1007/s00436-003-0963-x
  6. Chai JY, Park JH, Han ET, Guk SM, Lin A, Kim JL, Sohn WM, Yong TS, Eom KS, Min DY, Hwang EH, Phommasack B, Insisiengmay B, Rim HJ. Mixed infections with Opisthorchis viverrini and intestinal flukes in residents of Vientiane Municipality and Saravane Province in Laos. J Helminthol 2005; 79: 283-289. https://doi.org/10.1079/JOH2005302
  7. Chai JY, Han EK, Guk SM, Shin EH, Sohn WM, Yong TS, Eom KS, Lee KH, Jeong HG, Ryong YS, Hoang EH, Phommasack B, Insisiengmay B, Lee SH, Rim HJ. High prevalence of liver and intestinal fluke infection among residents of Savannakhet Province in Laos. Korean J Parasitol 2007; 45: 213-218. https://doi.org/10.3347/kjp.2007.45.3.213
  8. Sornmani S, Pathammavong O, Bunnag T, Impand P, Intarakhao C, Thirachantra S. An epidemiological survey of human intestinal parasites in Vientiane, Laos. Southeast Asian J Trop Med Pub Hlth 1974; 5: 541-546.
  9. Ditrich O, Scholz T, Giboda M. Occurrence of some medically important flukes (Trematoda: Opisthorchiidae and Heterophyidae) in Nam Ngum water reservoir. Southeast Asian J Trop Med Public Health 1990; 21: 482-488.
  10. Giboda M, Ditrich O, Scholz T, Viengsay T, Bouaphanh S. Human Opisthorchis and Haplorchis infections in Laos. Trans R Soc Trop Med Hyg 1991; 85: 538-540. https://doi.org/10.1016/0035-9203(91)90248-W
  11. Pholsena K, Sayaseng B, Hongvanthong B, Vanisaveth V. The prevalence of helminth infection in Ban Nanin, Laos. Southeast Asian J Trop Med Public Health 1991; 22: 137-138.
  12. Kobayashi J, Vannachone B, Xeutvongsa A, Manivong K, Ogawa S, Sato Y, Pholsena K. Prevalence of intestinal parasitic infection among children in 2 villages in Lao PDR. Southeast Asian J Trop Med Public Health 1996; 27: 562-565.
  13. Chai JY, Hongvanthong B. A small-scale survey of intestinal helminthic infections among the residents near Pakse, Laos. Korean J Parasitol 1998; 36: 55-58. https://doi.org/10.3347/kjp.1998.36.1.55
  14. Kobayashi J, Vannachone B, Sato Y, Manivong K, Nambanya S, Inthakone S. An epidemiological study on Opisthorchis viverrini infection in Lao villages. Southeast Asian J Trop Med Public Health 2000; 31: 128-132.
  15. Scholz T, Ditrich O, Giboda M. Larval stages of medically important flukes (Trematoda) from Vientiane Province, Laos. Part I. Metacercariae. Ann Parasitol Hum Comp 1990; 65: 238-243.
  16. Rim HJ, Sohn WM, Yong TS, Eom KS, Chai JY, Min DY, Lee SH, Hoang EH, Phommasack B, Insisengmay S. Fishborne trematode metacercariae detected in freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean J Parasitol 2008; 46: 253-260. https://doi.org/10.3347/kjp.2008.46.4.253
  17. Search FishBase: http://www.fishbase.org/search.php.
  18. Rim HJ. Opisthorchiasis. In Steele JH ed., CRC Handbook Series in Zoonoses, Section C: Parasitic Zoonoses, Vol. III (Trematode Zoonoses). Boca Raton, Florida, USA. CRC Press. 1982, p 109-121.
  19. Kaewkes S. Taxonomy and biology of liver flukes. Acta Trop 2003; 88: 177-186. https://doi.org/10.1016/j.actatropica.2003.05.001
  20. Thamavit W, Bhamarapravati N, Sahaphong S, Vajrasthira S, Angsubhakorn S. Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini infected Syrian golden hamsters. Cancer Res 1978; 38: 4634-4639.
  21. Vatanasapt V, Tangvoraphonkchai V, Titapant V, Pipitgool V, Viriyapap D, Sriamporn S. A high incidence of liver cancer in Khon Kaen Province, Thailand. Southeast Asian J Trop Med Public Health 1990; 21: 489-494.
  22. Pearson JC. A revision of the subfamily Haplorchinae Looss, 1899 (Trematoda: Heterophyidae). I. The Haplorchis group. Parasitology 1964; 54: 601-676. https://doi.org/10.1017/S003118200008269X
  23. Nath D, Pande BP. Identify of the three heterophyid metacercariae infesting some of the freshwater fishes. Current Sci India 1970; 39: 325-331.
  24. Velasquez CC. Observations on some Heterophyidae (Trematoda: Digenea) encysted in Philippine fishes. J Parasitol 1973; 59: 77-84. https://doi.org/10.2307/3278575
  25. Pande V, Premvati G. Development of metacercariae of Haplorchis spp. in chicks. Indian J Parasitol 1977; 1: 165-172.
  26. Sohn WM, Eom KS, Min DY, Rim HJ, Hoang EH, Yang Y, Li X. Fishborne trematode metacercariae in freshwater fish from Guangxi Zhuang Autonomous Region, China. Korean J Parasitol 2009; 47: 249-257. https://doi.org/10.3347/kjp.2009.47.3.249
  27. Pearson JC, Ow-Yang CK. New species of Haplorchis from Southeast Asia, together with keys to the Haplorchis-group of heterophyid trematodes of the region. Southeast Asian J Trop Med Public Health 1982; 13: 35-60.
  28. Pandey KC. Studies on metacercariae of freshwater fishes of India. 1. On the morphology of metacercaria of Haplorchis yokogawai (Katsuta, 1932) Chen, 1936. Proc Nat Acad Sci India, Sect. B 1966; 36: 437.
  29. Pande BP, Shukla RP. Metacercarial cyst of Haplorchis pumilio, it's development in experimental mammals and 2 other heterophyid infection of freshwater fishes and their zoonotic significance. Indian J Animal Sci 1972; 42: 971-978.
  30. Kliks M, Tantachamrun T. Heterophyid (Trematoda) parasites of cats in North Thailand, with notes on a human case found at necropsy. Southeast Asian J Trop Med Public Health 1974; 5: 547.
  31. Fahmy MA, Mandour AM, El-Naffar MK. Successful infection of dogs and cats by Prohemistomum vivax Sonsino, 1893 and Haplorchis yokogawai Katsuta, 1922. J Egyptian Soc Parasitol 1976; 6: 77.
  32. Premvati G, Pande V. On Centrocestus formosanus (Nishigori, 1924) Price, 1932 and it's experimental infection in White Leghorn chicks. Jpn J Parasitol 1974; 23: 79-84.
  33. Yanohara Y. On analysis of transmission dynamics of trematode infection. 1. Centrocestus formosanus infection in Miyakojima. Jpn J Parasitol 1985; 34: 55-70.
  34. Han ET, Shin EH, Phommakorn S, Sengvilaykham B, Kim JL, Rim HJ, Chai JY. Centrocestus formosanus (Digenea: Heterophyidae) encysted in the freshwater fish, Puntius brevis, from Lao PDR. Korean J Parasitol 2008; 46: 49-53. https://doi.org/10.3347/kjp.2008.46.1.49

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