• Applied Microscopy
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• v.39 no.3
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• pp.237-243
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• 2009

Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

• Applied Microscopy
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• v.39 no.3
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• pp.227-236
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• 2009

The ultrastructure of spermatogenesis and sperm in Coreoleuciscus splendidus, belonging to Gobioninae, Cyprinidae was investigated by light and electron microscopes. The testis was located between intestine and air bladder. The size of testis was major axis 1.8 cm, minor axis 3 mm. The testis of C. splendidus contained numerous testicular cysts, and spermatogenesis was non-synchronized in these testicular cysts. In May, the upper area of testis contained with other germ cells and sperm but the lower area of testis contained with matured sperm only. In case of spermatogonia, the nucleus was comparatively large spherical, and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged in a middle piece. The head of matured sperm was a spherical shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm had not lateral fins and 7 outer coarse fibers.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.6
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• pp.1220-1225
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• 1999

Differences in carotenoid composition in the integument of Korean bittering, Cheilognathus signifer and bride bittering, Rhodeus ukekii which are Korean native fresh water fish were compared. Total ca rotenoid contents in the integument of wild Korean bittering was 2.11mg% and composed of 42.6% zeaxanthin, 12.1% diatoxanthin and 12.1% lutein epoxide which are predominant carotenoids and 10.3% cynthiaxanthin, 8.3% zeaxanthin epoxide, 6.4% lutein and 1.5% cryptoxanthin which are minor carotenoids. Total ca rotenoid contents in the integument of wild bride bittering was 4.99mg% during a spawning period but after the spawning period it was decreased to 4.17mg% and carotenoid composition of bride bittering during the spawning period was 46.7% zeaxanthin, 26.5% diatoxanthin and 12.3% lutein which are predominant carotenoids, and 6.2% zeaxanthin epoxide, 3.1% cynthiaxanthin, 2.9% cryptoxanthin and 0.7% canthax anthin which are minor carotenoids. These results indicated that the carotenoid composition of bride bittering during spawning period was very similar to that of Korean bittering and carotenoid composition of bride bittering after the spawning period was 30.5% diatoxanthin, 21.5% cynthiaxanthin and 16.8% zeaxanthin which are predominant carotenoids and 14.0% cryptoxanthin, 11.3% lutein and 3.4% can thaxanthin which are minor carotenoids, indicating that after the spawning period, the content of zeaxanthin was decreased while that of cryptoxanthin and cynthiaxanthin was increased as compared to that of the spawning period. Total carotenoid contents in Korean bittering and bride bittering was relatively higher than that in other species of cyprinidae whereas composition of the carotenoid was similar.

• Korean Journal of Ecology and Environment
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• v.42 no.1
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• pp.134-138
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• 2009

The survival rate of PIT tagged fish was investigated for five Korean Cyprindae fish species, Acheilognathus lanceolatus (n=18, total length : $92.5{\pm}13.1mm$; body weight : $9.6{\pm}4.3g$), Hemibarbus labeo (n=28, TL : $220{\pm}74.4mm$; BW : $91.8{\pm}76.2g$), Zacco koreanus (n=13, TL : $116.5{\pm}23.8mm$; BW : $13.6{\pm}10.6g$), Zacco platypus (n=108, TL : $100.6{\pm}17.8mm$; BW: $8.7{\pm}4.8g$), Opsariichthys uncirostris amurensis (n=6, TL : $161.8{\pm}26.3mm$; BW : $27.5{\pm}18.3g$) with respect to applicability and effectiveness of PIT tagging. The survival rate were daily checked for 30 days. The survival rate was the highest and lowest for Z. koreanus and Z. platypus, respectively. The survival days were greater as fish total length increased. Based on these results, PIT tagging is not effective for Z. platypus, while PIT tagging for fish>150 mm was effect for field research.

• Korean Journal of Ichthyology
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• v.30 no.3
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• pp.161-166
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• 2018

The olfactory organ of Korean endemic fish Microphysogobio yaluensis are described anatomically and histologically, focused on relationship to its habitat and ecology. The paired olfactory organs are located at the dorsal snout, and externally consist of two semicircular nostrils and single nasal flap. They internally have rosette structure with 22 to 24 units of lamellae and the raphe inside the olfactory chamber. The lamella is made up of the sensory and the non-sensory epitheliums. The sensory epithelium has olfactory receptor neurons, supporting cells and basal cells whereas the nonsensory epithelium has stratified epithelial cells, ciliated non-sensory cells and mucous cells with acidic and neutral mucins. These structures might be considered that M. yaluensis has the olfactory organ which corresponds to the high sensitivity for its habitat and ecology, and is usable as a taxonomic key.

• Korean Journal of Ichthyology
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• v.18 no.2
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• pp.78-86
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• 2006

We examined the genetic diversity in intra-populations of Korean shiner, Coreoleuciscus splendidus, in six major rivers (Bukhan, Namhan, Geum, Osipcheon, Nakdong and Seomjin river) of Korea based on two different mitochondrial genes, the mitochondrial cytochrome b and the 16S rRNA. Analysis of sequence variation in a 657-bp segment of the mitochondrial cytochrome b gene revealed deep divergence among populations (98.2~99.9%) and high genetic diversity from geographically isolated populations. Intra-specific variation in this 697-bp segment of the 16S rRNA gene sequences was very low and nearly identical. Six isolate populations of C. splendidus showed a high similarity (97.7%~99.7%). This result may be indicative of a complex history of connection and isolation of the rivers in the Korea peninsula.

• Korean Journal of Ichthyology
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• v.29 no.4
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• pp.252-257
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• 2017

The oogenesis of the Microphysogobio yaluensis was investigated using light microscopy. Various developmental oocytes appeared in the ovary of the M. yaluensis. The oogenesis is largely divided into four stages: nuclear-chromatin stage, peri-nucleoli stage, vitellogenesis (yolk vesicle and yolk granule stages), and mature stage. The nuclear-chromatin is distributed in a large germinal vesicle as threads. The peri-nucleoli stage has many acidic nucleoli lining at the inner side of the nuclear membrane and an egg envelope just weakly starts. As the oogenesis gradually proceeds, they change to the vitellogenesis stage. The oocyte become to drastically increase and the marginal area of the ooplasm is covered with many vacuoles showing no negative reactions with hematoxylin and eosin staining, called the yolk vesicle stage. Many yolk vesicles-owned oocyte largely increase and as the development continues, its ooplasm is changed from the yolk vesicles to the yolk granules of eosinophilic. At the mature stage, lots of granules merged into a big yolk mass, acidophilic. Even at the mature stage, the egg envelope was still thin between the ooplasm and the follicular layer of the oocyte.

• Korean Journal of Ecology and Environment
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• v.42 no.3
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• pp.394-403
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• 2009

The fish fauna and community structures were investigated at 15 stations in the midupper region of the Seomjin River watershed from August in 2008 to April in 2009. During the studying period, 42 species belonging to 11 families were collected. Dominant family in the all sites was Cyprinidae (28 species, relative abundance: 66.7%) during the study. Total of 42 species (primary freshwater: 40 species, peripheral freshwater: 2 species) were found with the primary freshwater fishes being the highest. Dominant species was Zacco platypus (22.3%), and subdominant species was Coreoferea herzi (10.8%). Also, Pseudogobio esocinus (9.0%) and Carassius auratus (5.8%) were numerous. There were 17 Korean endemic species (40.5%), including Rhodeus ocellatus, Acheilgnathus koreensis, A. somjinensis, Coreoleuciscus splendidus and Sarcocheillichthys variegatus wakiyae. Among them, Acheilgnathus somjinensis was identified for designation required to protect under a wildlife category. In conclusion, we could define that the Seomjin River watershed has a very good water conditions as habitat of fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.5
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• pp.436-448
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• 1984

The taxonomy of gudgeons of the subfamily Gobioninae in Korea is reviewed and keys to supraspecific taxa (subfamilies and genera) of the subfamily Gobioninae are provided, as well as the synonyms and distribution of the species or subspecies. Abbottina springeri, poorly known in Korea is redescribed with the extention of its known morphological characters and geographical distribution. Squalidus sp. occurring rivers of the Yongdokoship, the Hyongsan, and the Hoeya in the Gyongsangbugdo and in the Gyongsangnamdo differs from three previously described species from Korea in the numbers and structure of lateral line scales, color patterns on the body sides, and eye diameter etc. The genus name of Gonoprcktopterus mylodon should be transferred from Gonoproktopterus to Hemibarbus, because the species is more similar to Hemibarbus longirostris or Belligobio eristrigma than to Gonoproktopterus, an Indian genus of Barbinae. The korean gudgeons of the subfamily Gobioninae are grouped five possible phyletic series, as follow ; Hemibarbus, Pseudorasbora, Sarcocheilichthys, Gnathopogon, and Pseudogobio group. The genus Gobiobotia is not placed in the subfamily Gobioninae but in the subfamily Gobiobotinae in relation to 3 pairs of mental barbels and some osteological characters.

• Korean Journal of Ichthyology
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• v.31 no.2
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• pp.101-109
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• 2019

This study investigated the characteristics of fish communities at the Lake Yedang, in 2013 to 2014 and 2017. During the survey period, there were 29 species of 10 families collected from 3 survey stations. Of them, Cyprinidae occupied 34.5% (14 species), Bagridae, Gobiidae 10.3% (3 species), and Cobitidae, Centrachidae were 6.9% (2 species) in the number of species, respectively. Korean endemic species were 4 species (13.8%) Abbottina springeri, Cobitis nalbanti, Pseudobagrus koreanus, Odontobutis interrupta and accounted for a low rate. There were 3 species (10.3%) exotic species including Carassius cuvieri, Lepomis macrochirus, Micropterus salmoides. As a result of community analysis, dominant index was 0.45~1.00, diversity index was 0.19~2.30 and richness index was 0.41~1.94.