Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Oogenesis of Microphysogobio yaluensis (Pisces, Cyprinidae) in the Korean Endemic Species

한국고유종 돌마자의 난자형성과정

  • Kim, Jae Goo (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Reu, Dong Suck (Department of Life Science, Cheongju University) ;
  • Park, Jong Yong (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University)
  • 김재구 (전북대학교 자연과학대학 생물학과, 전북대학교 생물다양성연구소) ;
  • 류동석 (청주대학교 이공대학 생명과학과) ;
  • 박종영 (전북대학교 자연과학대학 생물학과, 전북대학교 생물다양성연구소)
  • Received : 2017.09.14
  • Accepted : 2017.12.01
  • Published : 2017.12.31
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Abstract

The oogenesis of the Microphysogobio yaluensis was investigated using light microscopy. Various developmental oocytes appeared in the ovary of the M. yaluensis. The oogenesis is largely divided into four stages: nuclear-chromatin stage, peri-nucleoli stage, vitellogenesis (yolk vesicle and yolk granule stages), and mature stage. The nuclear-chromatin is distributed in a large germinal vesicle as threads. The peri-nucleoli stage has many acidic nucleoli lining at the inner side of the nuclear membrane and an egg envelope just weakly starts. As the oogenesis gradually proceeds, they change to the vitellogenesis stage. The oocyte become to drastically increase and the marginal area of the ooplasm is covered with many vacuoles showing no negative reactions with hematoxylin and eosin staining, called the yolk vesicle stage. Many yolk vesicles-owned oocyte largely increase and as the development continues, its ooplasm is changed from the yolk vesicles to the yolk granules of eosinophilic. At the mature stage, lots of granules merged into a big yolk mass, acidophilic. Even at the mature stage, the egg envelope was still thin between the ooplasm and the follicular layer of the oocyte.

잉어목 모래무지아과에 속하는 한국 고유종 돌마자 Microphysogobio yaluensis 난소 내 생식세포들의 형태학적 특징을 연구하기 광학현미경을 이용하여 조사하였다. 난자형성과정 (oogenesis)은 크게 염색인기 (chromatin-nucleolus stage), 주변인기(peri-nucleolus stage), 난황형성기(vitellogenesis)의 난황포 및 난황구기와 성숙(mature stage)의 단계로 구분되었다. 염색인기에는 배포가 크게 형성되며 실모양의 염색질이 산재되어 있다. 주변인기에는 핵 내에 산성의 인들이 핵막인근에 분포하고 있었으며, 난막(egg envelope)이 형성되기 시작하였다. 이후 난황형성기의 난황포 단계에서는 세포질의 대부분이 텅빈 공포모양의 난황포로 구성되며, 발생이 진행되면서 난황구 단계에서는 난황포 사이에 eosin에 염색되는 난황과립으로 대체되었다. 성숙단계에 도달한 난세포에는 많은 난황구들이 하나의 커다란 난황괴(yolk mass)를 형성하고 있었다. 이 시기의 난세포의 난막은 세포질과 여포세포층 사이에 얇게 형성되었다.

Keywords

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