Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

A molecular Genetic Variation among Intra-poplations of Korean shiner, Coreoleuciscus splendidus Mori (Cyprinidae)

한국산 쉬리, Coreoleuciscus splendidus (잉어과)의 종내 집단간 분자 유전 변이

  • Song, Ho-Bok (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Park, Gab-Man (Department of Parasitology, Kwandong University College of Medicine)
  • 송호복 (강원대학교 자연과학대학 생명과학부) ;
  • 박갑만 (관동대학교 의과대학 의학과)
  • Received : 2006.02.03
  • Accepted : 2006.05.24
  • Published : 2006.06.30
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Abstract

We examined the genetic diversity in intra-populations of Korean shiner, Coreoleuciscus splendidus, in six major rivers (Bukhan, Namhan, Geum, Osipcheon, Nakdong and Seomjin river) of Korea based on two different mitochondrial genes, the mitochondrial cytochrome b and the 16S rRNA. Analysis of sequence variation in a 657-bp segment of the mitochondrial cytochrome b gene revealed deep divergence among populations (98.2~99.9%) and high genetic diversity from geographically isolated populations. Intra-specific variation in this 697-bp segment of the 16S rRNA gene sequences was very low and nearly identical. Six isolate populations of C. splendidus showed a high similarity (97.7%~99.7%). This result may be indicative of a complex history of connection and isolation of the rivers in the Korea peninsula.

한국산 쉬리, Coreuleuciscus splendidus의 종내 집단간 유전자 다양성을 알기 위해 6개 주요강(북한강, 남한강, 금강, 오십천, 낙동강, 섬진강)으로부터 채집된 개체를 대상으로 16S rRNA 유전자와 미트콘드리아 cytochrome b 유전자에 근거하여 비교 분석하였다. 미트콘드리아 cytochrome b 유전자의 657 bp 길이의 염기서열 분석결과, 6개 집단간에 차이는 98.2~99.9%로 나타났으며 지리적으로 격리된 집단간에 높은 유전적 다양성을 보였다. 16S rRNA 유전자는 697 bp의 염기서열을 얻었으며, 종내 변이는 큰 차이가 없이 거의 동일하였다. 16S rRNA 유전자의 6개 집단간에는 97.7%에서 99.7%의 높은 유사성을 보였다.

Keywords

Acknowledgement

Supported by : Kwandong University

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