• Korean Parent-Child Health Journal
• /
• v.8 no.1
• /
• pp.49-63
• /
• 2005

Purpose: The present study is a descriptive research on the content of sex education conducted at home by the mothers of young children who are attending five kindergartens located in Busan, Korea. This study aims to provide basic materials for systematic sex education at home and in the school. Method: Based on the literature review and prior researches, the writer made a questionnaire consisting of 30 items regarding necessities, contents and difficulties of sex education. The 307 questionnaires were collected out of 480 which were distributed by the kindergarten teachers. Data was analysed with frequency and percentage using the SPSS Win 10.0 Program. The main subjects of the study were mothers in their thirties, who understood the necessity of sex education. However, they were lack of enough sexual knowledge in accordance with the transforming sexual culture and lack of suitable materials. They did not plan to educate their children according to their developmental stages, but just taught them verbally rather than using common materials for the situation. Result Regarding the origin of life that children often asks, mothers did not give a scientific and correct response to satisfy their curiosity about sex, saying that a baby was created after marrying a loved one. While more mothers than the prior research responded correctly to the queries of the path of delivery, some mothers did not. It may cause some children to suppress the questions, which may result a distorted sexual knowledge. That is because mothers understood that sex education was to deliver sexual knowledge, but not realized that sex education was a human education carried out throughout life. Mothers fell short of sexual knowledge, capability, and confidence, comparing with swift social changes and children's capacity. As a result, it is necessary to inspire sexual awareness into mothers and educate them through training. It is also needed to secure a training program similar to a teacher training program. Conclusion: It is essential to develop and to provide evaluation tools as well as proper materials in accordance with the children's developmental stage, so that organic management system among home, education institute and society must lead sex education Further, it is necessary to provide teaching methods and materials to mothers regularly, so that the school and home may conduct sex education together to cope with difficulties.

• Korean Journal of Plant Resources
• /
• v.29 no.1
• /
• pp.32-38
• /
• 2016

In order to improved use of Persicaria tinctoria seeds and to get basic information, general composition, fatty acid, mineral, amind acid analysis and physiological activity of methanol extract of Persicaria tinctoria seeds were investigated. Total calories of Persicaria tinctoria seeds were 348.00 kcal/100 g, general composition, carbohydrate, crude protein, crude fat and crude ash consisted of 7.85%, 67.90%, 10.10%, 4.00% and 10.15%, respectively. The amount of saturated and unsaturated fatty acids was showed 0.9048 g/100 g and 2,714 g/100 g, respectively. Minerals contained 100g of Persicaria tinctoria seeds were followed by K (549.5 ㎎), Mg (264.4 ㎎), Ca (216.2 ㎎), Fe (12.1 ㎎), Zn (3.0 ㎎). Total 15 kinds of amino acids were detected, these amino acids displayed higher value in the alanine (1,432.6 ㎎/100 g) and glutamic acid (1,088.8 ㎎/100 g). Total polyphenol and flavonoid contents were 11.08 ㎎/L and 3.56 ㎎/L, respectively. DPPH radical scavenging and ABTS radical scavenging activity in the methanol extract of 1,000 ㎎/L was showed 86.74% and 61.74%, respectively.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.9
• /
• pp.893-902
• /
• 2011

Endophytic fungi are little known for their role in gibberellins (GAs) synthesis and abiotic stress resistance in crop plants. We isolated 10 endophytes from the roots of field-grown soybean and screened their culture filtrates (CF) on the GAs biosynthesis mutant rice line - Waito-C. CF bioassay showed that endophyte GMH-1B significantly promoted the growth of Waito-C compared with controls. GMH-1B was identified as Penicillium minioluteum LHL09 on the basis of ITS regions rDNA sequence homology and phylogenetic analyses. GC/MS-SIM analysis of CF of P. minioluteum revealed the presence of bioactive $GA_4$ and $GA_7$. In endophyte-soybean plant interaction, P. minioluteum association significantly promoted growth characteristics (shoot length, shoot fresh and dry biomasses, chlorophyll content, and leaf area) and nitrogen assimilation, with and without sodium chloride (NaCl)-induced salinity (70 and 140 mM) stress, as compared with control. Field-emission scanning electron microcopy showed active colonization of endophyte with host plants before and after stress treatments. In response to salinity stress, low endogenous abscisic acid and high salicylic acid accumulation in endophyte-associated plants elucidated the stress mitigation by P. minioluteum. The endophytic fungal symbiosis of P. minioluteum also increased the daidzein and genistein contents in the soybean as compared with control plants, under salt stress. Thus, P. minioluteum ameliorated the adverse effects of abiotic salinity stress and rescued soybean plant growth by influencing biosynthesis of the plant's hormones and flavonoids.

• Microbiology and Biotechnology Letters
• /
• v.42 no.4
• /
• pp.354-360
• /
• 2014

In this study, the anti-obesity activity of Smilax china methanol extract (SCME) was evaluated using a pancreatic lipase enzyme inhibition assay, and a cell culture model system. Results indicated that, SCME effectively inhibited pancreatic lipase enzyme activity in a dose-dependent manner. Furthermore, SCME significantly suppressed insulin, dexamethasone, 3-isobutyl-1-methylxanthine-induced adipocyte differentiation, lipid accumulation, and triglyceride contents on 3T3-L1 preadipocytes, in a dose-dependent manner. The anti-adipogenic effect was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (C/EBP) ${\alpha}$, $C/EBP{\beta}$, and the peroxisome proliferator-activated receptor ${\gamma}$ gene and protein expressions. Moreover, SCME triggered lipolysis effects dose-dependently on adipocyte. Taken together, these results provide an important new insight into SCME, indicating that it possesses anti-obesity activity through pancreatic lipase inhibition, anti-adipogenic and lipolysis effects. SCME may therefore be utilized as a promising source in the field of nutraceuticals. The identification of active compounds that confer the anti-obesity activities of SCME may be a logical next step.

• Microbiology and Biotechnology Letters
• /
• v.42 no.3
• /
• pp.249-257
• /
• 2014

In this study, the anti-oxidative and anti-obesity activities of Amomum cardamomum L. methanol extract (ACME) were evaluated using DPPH radical scavenging activity assay, pancreatic lipase enzyme inhibition assay, and the cell culture model system. ACME exhibited DPPH radical scavenging activities dose-dependently, with $IC_{50}$ of DPPH radical scavenging activities of ACME being $25.15{\mu}g/ml$. Furthermore, ACME effectively suppressed pancreatic lipase enzyme activity dose-dependently. ACME also significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents, and triggered lipolysis activity on 3T3-L1 preadipocytes in a dose-dependent manner, without cytotoxicity. Their anti-obesity effect was modulated by the cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$ and the peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) gene and protein expressions. Taken together, these results provide an important new insight that A. cardamomum L. possesses anti-oxidative and anti-obesity activities such as pancreatic lipase inhibition, anti-adipogenic, and lipolysis effects. There is therefore potential for its use as a promising component in the field of nutraceuticals and the identification of the active compounds that confer the anti-oxidative and anti-obesity activities of ACME might be an appropriate next step.

• Journal of Ginseng Research
• /
• v.40 no.1
• /
• pp.38-46
• /
• 2016

Background: Blind and excessive application of fertilizers was found during the cultivation of Panax notoginseng in fields, as well as increase in root rot disease incidence. Methods: Both "3414" application and orthogonal test designs were performed at Shilin county, Yunnan province, China, for NPK (nitrogen, phosphorus, and potassium) and mineral fertilizers, respectively. The data were used to construct the one-, two-, and three-factor quadratic regression models. The effect of fertilizer deficiency on root yield loss was also analyzed to confirm the result predicted by these models. A pot culture experiment was performed to observe the incidence rate of root rot disease and to obtain the best range in which the highest yield of root and saponins could be realized. Results: The best application strategy for NPK fertilizer was $0kg/667m^2$, $17.01kg/667m^2$, and $56.87kg/667m^2$, respectively, which can produce the highest root yield of 1,861.90 g (dried root of 100 plants). For mineral fertilizers, calcium and magnesium fertilizers had a significant and positive effect on root yield and the content of four active saponins, respectively. The severity of root rot disease increased with the increase in soil moisture. The best range of soil moisture varied from 0.56 FC (field capacity of water) to 0.59 FC, when the highest yield of root and saponins could be realized as well as the lower incidence rate of root disease. Conclusion: These results indicate that the amount of nitrogen fertilizer used in these fields is excessive and that of potassium fertilizer is deficient. Higher soil moisture is an important factor that increases the severity of the root rot disease.

• Journal of Korean Home Economics Education Association
• /
• v.20 no.2
• /
• pp.61-73
• /
• 2008

The purpose of this study was to improve interest and attitude of student's Technology-Home Economics Curriculum and to recognize the change of recognition on Traditional image through Natural Dyeing and Jogakbo making on the unit of making living goods with fabrics in Technology-Home Economics curriculum on first year high school. The abridged result of study following this: First, in Natural Dyeing and Jogakbo making, teaching-learning plan applying LT cooperative studying and learning materials have been developed. Second, after applying the developed lesson plan, the result showed that the change in learning interest and attitude about Technology-Home Economics curriculum was positively improved. After executing the class on Natural Dyeing and Jogakbo making, the interest and concern about tradition were very positively upgraded through the result analyzing the change of recognition on traditional image. Through these results, The Teaching-learning plan and learning materials would show high possibility of application as educational contents about traditional culture in the field of education. After executing the class on making of Natural dyeing and Jogakbo, the learning interest degree and attitude was very positively upgraded, and the recognition on traditional image had been changed to the active and positive recognition.

• Journal of Korean Home Economics Education Association
• /
• v.31 no.4
• /
• pp.63-79
• /
• 2019

The purpose of this study was to suggest valuable maker education programs by implementing and evaluating a Home Economics(HE) maker education program developed based on the content of "Hanbok and Creative Clothing" for high school students. The results of this study are as follows. First, the HE maker education model for high school students was designed and developed. The HE maker education model was developed by integrating and modifying the TMSI model of the maker education model and Laster's HE practical action teaching model. The HE maker education model consisted of 4 steps: tinkering(T: 4-hour class), practical reasoning(P: 3-hour class), making together(M: 4-hour class), and sharing and spreading(S: 1-hour class) with a total of 12-hour lesson plans. The theme of the developed HE maker program is 'Practice and spread of creative traditional culture of life (Hanbok)'. Second, the results of online survey of 240 high school students who participated in this maker class showed that HE maker class had positive effects in the order of experiential(4.26), cognitive(4.22), emotional(4.18), social(4.18), and practical(4.10). It is expected that the findings of this study will contribute to diversifying the curriculum of Home Economics, thereby improving the quality of Home Economics Education.

• KSBB Journal
• /
• v.10 no.4
• /
• pp.349-356
• /
• 1995

To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were $37^{\circ}C$ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.

• Journal of Bio-Environment Control
• /
• v.20 no.4
• /
• pp.311-319
• /
• 2011

Overusing chemical fertilizers involves potassium accumulation in the soil, which can become a toxicity problem in agriculture. This study was conducted to investigate the effect of potassium (K) treatment on growth, physiological characteristics, and morphological changes using Chinese cabbage (Brassica rapa L. ssp. campestris). With high (600 mM) K treatment, the plant growth traits of leaf length, leaf area, and fresh and dry weight of shoots and roots decreased, whereas chlorophyll content increased. As the concentration of K increasing, total N, P, and K increased in leaves, but concentrations of Ca, Mg, and Na decreased. However, Mn, Fe and Zn contents were highest in 100 mM K treatment. Chlorophyll a, b, and carotenoids increased with increasing K concentration. Maximum photochemical efficiency ($F_v/F_m$) was not significant in the all treatments, whereas $CO_2$ assimilation decreased with increasing K level due to stomatal degradation. Total free amino acids increased with the 10 and 100 mM K but decreased at 600 mM K treatments. Therefore, the growth and physiological characteristics of Chinese cabbage ascertained that tolerance up to 100 mM K when grown with nutrient solution in pot culture.