• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.282-287
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• 2011

Bacterial pustule (BP) is a leaf disease of soybean that is most common in Korea. Inoculation of 8ra, pathogen strain, to resistant and susceptible cultivars for finding the BP resistance gene (rxp) was much tried but the sequence of the exact gene is not found. This research performed in order to confirm the rxp gene near molecular marker by using the resistant and susceptible cultivars. Soybean BP resistance gene which related to region of near molecular marker could select the resistant cultivar. For the near molecular marker of rxp, reference genomics data available at sequenced Phytozome was used for designing molecular markers. The rxp was mapped between Satt372 and Satt486 on chromosome 17. According to previous study, rxp released in find mapping 7.2 Mbp to 7.3 Mbp on chromosome 17. In this study, we developed 3 random markers near from 6.6 Mbp to 7.3 Mbp on chromosome 17 identified to increase the genetic resolution of the rxp gene region using resistant and susceptible cultivars. Particularly, Rxp17-700 marker was mostly coincided resistance and susceptible genotype to rxp. This result suggests that Rxp17-700 marker will be more tightly linked to rxp gene.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.664-668
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• 2009

"Boram" is a new $F_1$ hybrid of rapeseed (Brassica napus L.) with early maturing, high yield and high oleic acid. This cultivar was developed from the cross between Mokpo-CGMS (male steile line) and 8634-B-1-3-1-8 (restore line) for the production of edible oil and biodiesel in 2006. Yield trials were conducted from 2007 to 2008 and regional adaptation trials were examined at four locations in 2008. "Boram" has green and parted leaf, yellowish flower, and black seed coat. The ripening period of "Boram" is similar to "Sunmang", but 3 days faster than "Tammiyuchae". The average seed yield of the regional adaptation trials was 424 kg/10a, which was 11 and 48% higher than "Sunmang" and "Tammiyuchae" variety. Total oil content of "Boram" was 44.2%. In fatty acid composition, "Boram" has no erucic acid. However, oleic acid content is 68.5%, which is 3.7 and 5.4% higher than "Tammiyuchae" and "Sunmang". And total glucosinolate content was 2.27mg/g. Therefore this variety is recommended as a leading variety at southwestern areas including Jeonnam, Jeonbuk and Kyeongnam provinces and Jeju island of Korea.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.220-223
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• 2010

A FA intersectional hybrid lily cultivar 'Cherry Pink' was developed in 2006 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) Korea. The cross and immature embryo rescue was conducted between female parents Lilium FA hybrid 'Supia' and male parents L. Asiatic hybrid 'A94-9' by cut style pollination method (CSM) at Suwon in 1999. The first selection was done and was tentatively named as 'FA01-17' in 2001. After in vitro multiplication, bulbing production of 'FA01-17' line, growth and flowering characteristic tests were conducted from 2002 to 2005. The evaluation of characteristics and consumer preferences were surveyed at a lily flower show of NIHHS at Taean in 2006. 'Cherry Pink' flowered in the middle of June and grew more than 120 cm stem in length. Flowers bloomed facing upward, a little spotted in petals and deep pink (RHS, RP27D). The pollen of 'Cherry Pink' was sterile. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. It was needed to control Botrytis disease in wet season.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.3
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• pp.246-268
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• 2019

Soybean [Glycine max (L.) Merr.] is a species of legume native to East Asia. The interactions between climatic conditions and genetic characteristics are known to affect the agricultural performance of soybean. Therefore, the present investigation was conducted to identify the main elements affecting the agricultural performances of 11 soybean varieties/lines from China [Harbin ($45^{\circ}12^{\prime}N$), Yanji ($42^{\circ}53^{\prime}N$), Dalian ($39^{\circ}30^{\prime}N$), Qingdao ($36^{\circ}26^{\prime}N$)] and the Republic of Korea [Suwon ($37^{\circ}16^{\prime}N$), and Jeonju ($35^{\circ}49^{\prime}N$)]. The days to flowering (DTF) of soybeans with the e1-nf and e1-as alleles and the E1e2e3e4 genotype, except in 'Keumgangkong', 'Tawonkong', and 'Duyoukong', were relatively short compared to those of soybeans with other alleles. Although DTF of the soybeans was highly correlated with all climatic conditions [negative: precipitation, average temperature (AVT), accumulated temperature; positive: day-length (DL)], days to maturity and 100-seed weight of the soybeans showed no significant correlation with any climatic conditions. The soybeans with a dominant Dt1 allele, except 'Tawonkong', had the longest stem length (STL). Moreover, STL of the soybeans grown in the test fields showed a positive correlation with only DL; however, the results of our chamber test that was conducted to complement the field tests showed that STL of soybean was positively affected by AVT and DL. Although soybean yield (YLD) showed positive correlations with latitude and DL (except L62-667, OT89-5, and OT89-6), the response of YLD to the climatic conditions was cultivar-specific. Our results show that DTF and STL of soybeans grown in six different latitudes are highly affected by DL, and AVT and genetic characteristic also affect DTF and STL.

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.375-382
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• 2007

The vascular plants of the studied area in the Mt. Seokbul appeared to be 342 taxa; 96 families, 249 genera, 293 species, 2 subspecies, 43 varieties and 3 forms and 1 cultivar. Based on the list of the rare plants by the Forest Research Institute, 2 taxa existed in the studied areas; Lloydia triflora(Preservation priority order; No. 169), Aristolochia contorta(No. 151) and based on the list of Korean endemic plants, 7 taxa existed; Cephalotaxus koreana, Carex okamotoi, Populus tomentiglandulosa(Planted species), Indigofera koreana, Weigela subsessilis. Rhododendron yedoense var. poukhanense, Forsythia koreana (Planted species). Specific plant species by floral region were total 18 taxa; Wistaria floribunda(Planted species) in class IV, Lloydia triflora, Acer palmatum in class III, 15 taxa (Cephalotaxus koreana, Quercus variabilis, Ulmus parvifolia, Aristolochia contorta, Pyrus ussuriensis, etc.) in class I. The naturalized plants in this site were 11 families, 26 genera, 32 species, 2 varieties, 34 taxa, and naturalization rate was 9.9% of all 342 taxa vascular plants. The plant communities in the Mt. Seokbul were classified as; Pinus densiflora community(No. 1, 3, 4) and Pinus thunbergii community(No.2).

• Korean Journal of Plant Resources
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• v.21 no.3
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• pp.178-183
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• 2008

The growth and yield performance of 19 new com hybrids were evaluated. Results showed that all hybrids had a superior growth performance in the drained-paddy than in the upland field except for daeyul ${\times}$ cheongdo and hyoryeong ${\times}$ cheongdo in plant height, cheongdoyeop ${\times}$ wx-3 in tassel1ength, and cheongdo (black) ${\times}$ wx-3 in number of tassel branch. The same hybrids, except cheongdoyeop ${\times}$ wx-3, obtained lower ear quality in drained-paddy field compared to upland in terms of ear weight, and ear and grain setting length. The highest yield in the drained-paddy and upland fields was obtained in the hybrids ks5wx ${\times}$ ks6wx ${\times}$ cheongdo (1,633.3kg ${\cdot}$ $10a^{-1}$) and daeyul ${\times}$ wx-3 (1,516.7kg ${\cdot}$ $10a^{-1}$), respectively. Highest yield among the wx-3 crosses was obtained in daeyul which was 1,583.3kg ${\cdot}$ $10a^{-1}$ and 1,516.7kg ${\cdot}$ $10a^{-1}$ in drained-paddy and upland field, respectively. For the crosses of wx-8, highest yields were recorded in the cultivar bugye50 (1,466.7kg ${\cdot}$ $10a^{-1}$) and seokgu12 (1,384.6kg ${\cdot}$ $10a^{-1}$) for drained-paddy and upland field, respectively. In the case of cheongdo, highest yields were obtained in ks5wx ${\times}$ ks6wx (1,633.3kg ${\cdot}$ $10a^{-1}$) and seokgu14 (1,111.1kg ${\cdot}$ $10a^{-1}$) for drained-paddy and upland field, respectively. Result also showed that the drained-paddy soil had better physicochemical properties than the upland. The relatively high performance in terms of growth parameters and yield of com hybrids planted in the drained-paddy field is in agreement with the higher organic matter and micro-element content of drained-paddy field.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.4
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• pp.177-181
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• 1975

The pot experiment was conducted primarily to clarify the relationship between lime application and they amount of potassium for the basal and top dressings on paddy rice. (Orizae sativa L. cultivar; variety Jinheung) The soils mixed with lime and without lime were separately filled in to 0.2827 a. open-bottom concretepots. The soil with lime was treated with slaked lime, at two weeks before transplanting to correct soil pH. around 7.0. The total amount of potassium 12 kilograms per 10a was applied by two or three split doses during transplanting, 19 days after transplanting and primodial stages. The results of experiment obtained are summarized as follows: 1. The application of potash in the rate of 4kg per 10a or less for basal dressing and the remaining amount of potash for top dressing at 19 days after transplanting and primodial stage or one top dressing, at primodial stage have increased the yield of paddy with increased number of panicles per hill, milliequivalent ratio of $K^-/Ca^{++}+Mg^{++}$ in leaves in the later stage of growth, number of ripened grains per panicle, and rate of grain maturity. 2. On the other hand, the greater the amount of potassium given for the basal dressing (over 4kilograms per 10a), the smaller number of tillers and panicles were resulted. And the tendency was considered chiefly due to high salt concentration at the early stage of rice growth.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.278-286
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• 2021

Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.431-448
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• 2021

We decided the efficient application timings of organo-synthetic insecticides for controlling the first generation larvae of O. furnacalis through investigations of insect stage-specific densities, damage aspects in maize, and effects of insecticides. A waxy maize cultivar, Ilmichal, was cultivated from April 20 (sowing) to July 26 (harvest, dough stage of maize) in Suwon, 2016. The maximum and 50% cumulative catch dates of the overwintering generation adults in the sex pheromone trapping were May 29 and May 31, respectively. Most of the first generation larvae finished their occurrence till the early reproductive stage of maize. The first generation larvae fed on leaves inside the whorl leaves before tassel and stem development of maize, sequentially moved to tassel and stem, and then moved finally to stem and ear parts. In the results of insecticide applications at different dates, the 9-11 leaf stage (June 10~17) and the 6-7 leaf stage (June 3) of maize were the most efficient application timings for direct spray of Etofenprox EC to maize, and for application of Carbofuran granules onto soil surface, respectively, which resulted in suppression of tunnelling damages. The timings for the two insecticides were 12-19 days and 5 days after the adult maximum catch date, respectively. Those timings after the 50% cumulative adult catch date were advanced 2 days.

• Genes and Genomics
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• v.40 no.12
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• pp.1319-1329
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• 2018

SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.