Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development of Molecular Markers Conferring Bacterial Leaf Pustule Resistance Gene, rxp, using Resistant and Susceptible Cultivars in Soybean

콩 불마름병 저항성 및 감수성 품종을 이용한 rxp 유전자 근접 분자표지 개발

  • 양기웅 (국립식량과학원 기능성작물부) ;
  • 이영훈 (국립식량과학원 기능성작물부) ;
  • 고종민 (국립식량과학원 기능성작물부) ;
  • 전명기 (국립식량과학원 기능성작물부) ;
  • 이병원 (국립식량과학원 기능성작물부) ;
  • 김현태 (국립식량과학원 기능성작물부) ;
  • 윤홍태 (국립식량과학원 기능성작물부) ;
  • 정찬식 (국립식량과학원 기능성작물부) ;
  • 백인열 (국립식량과학원 기능성작물부)
  • Received : 2011.05.09
  • Published : 2011.09.30
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Abstract

Bacterial pustule (BP) is a leaf disease of soybean that is most common in Korea. Inoculation of 8ra, pathogen strain, to resistant and susceptible cultivars for finding the BP resistance gene (rxp) was much tried but the sequence of the exact gene is not found. This research performed in order to confirm the rxp gene near molecular marker by using the resistant and susceptible cultivars. Soybean BP resistance gene which related to region of near molecular marker could select the resistant cultivar. For the near molecular marker of rxp, reference genomics data available at sequenced Phytozome was used for designing molecular markers. The rxp was mapped between Satt372 and Satt486 on chromosome 17. According to previous study, rxp released in find mapping 7.2 Mbp to 7.3 Mbp on chromosome 17. In this study, we developed 3 random markers near from 6.6 Mbp to 7.3 Mbp on chromosome 17 identified to increase the genetic resolution of the rxp gene region using resistant and susceptible cultivars. Particularly, Rxp17-700 marker was mostly coincided resistance and susceptible genotype to rxp. This result suggests that Rxp17-700 marker will be more tightly linked to rxp gene.

본 연구는 최근 콩 재배에서 심각한 병으로 대두된 콩 불마름병에 대한 저항성 유전자인 rxp 근접분자표지를 개발하고자 수행하였다. 콩 불마름병은 국내에서 전국적으로 발생하는 심각한 세균병으로 이에 관련하여 세균병 접종을 이용한 저항성 품종과 감수성 품종에 대한 연구가 많이 진행되고 있지만 정확한 유전자의 염기서열이 밝혀져 있지 않고 있다. 본 연구는 콩 불마름병에 저항성 품종 8개체와 감수성 품종 8개체를 이용하여 rxp 유전자 근접분자표지를 확인하기 위하여 수행하였다. 콩 불마름병 저항성 유전자로 알려진 rxp 유전자는 chromosome 17의 Satt486과 Satt372 사이에 있다고 알려져 있으며, 최근 연구결과로 chromosome 17의 7.27-7.30 Mbp 사이에 있다. 연구진은 chromosome 17의 6.6-7.3 Mbp 사이에 random으로 분자표지를 제작하여 저항성과 감수성 품종에서 다형성을 알아보았다. 실험결과로 콩 불마름병관련 근접분자표지 3점을 개발하였고, Rxp17-700 분자표지는 흥미로운 rxp 근접분자표지 임을 확인하였다. 이러한 콩 불마름병 저항성관련 근접분자표지는 앞으로 저항성 품종을 선발하는데 도움이 될 것이다.

Keywords

Acknowledgement

Supported by : 국립식량과학원

References

  1. Choi IY, Hyten DL, Matukumalli LK, Song Q, Chaky JM, Quigley CV, Chase K, Lark KG, Reiter RS, Yoon MS, Hwang EY, Yi SI, Young ND, Shoemaker RC, van Tassell CP, Specht JE, Cregan PB. 2007. A soybean transcript map: gene distribution, haplotype and single-nucleotide polymorphism analysis. Genetics 176:685-696. https://doi.org/10.1534/genetics.107.070821
  2. Chu Z, Fu B, Yang H, Xu C, Li Z, Sanchez A, Park YJ, Bennetzen JL, Zhang Q, Wang S. 2006 Targeting xa13, a recessive gene for bacterial blight resistance in rice. Theor. Appl. Genet. 112:455-461. https://doi.org/10.1007/s00122-005-0145-6
  3. Fehr WR, Caviness CE, Burmood DT, Pennington JS. 1971. Stages of development descriptions for soybeans, Glycines Max(L.) Merrill. Crop Sci. 11:929-931. https://doi.org/10.2135/cropsci1971.0011183X001100060051x
  4. Hartwig EE, Johnson HW. 1953. Effect of the bacterial pustule on yield and chemical composition of soybeans. Agron J. 45:22-23. https://doi.org/10.2134/agronj1953.00021962004500010005x
  5. Hartwig EE, Lehman SG. 1951. Inheritance of resistance to the bacterial pustule disease in soybeans. Agron. J. 43:226-229. https://doi.org/10.2134/agronj1951.00021962004300050005x
  6. Kim KS. 2003. Development of molecular markers for resistance gene to Xanthomonas axonopodis in soybean. Dissertation, Seoul National University
  7. Kim DH, Kim KH, Van K, Kim MY, Lee SH. 2010. Fine mapping of a resistance gene to bacterial leaf pustule in soybean. Theor. Appl. Genet. 120:1443-1450. https://doi.org/10.1007/s00122-010-1266-0
  8. Kim KS, Van K, Kim MY, Lee SH. 2004. Development of molecular markers for Xanthomonas axonopodis resistance in soybean. Korean J. Crop Sci. 49:429-433.
  9. Kim KH, Kim MY, Van K, Moon JK, Kim DH, Lee SH. 2008. Marker-assisted foreground and background selection of near isogenic lines for bacterial leaf pustule resistant gene in soybean. J. Crop Sci. Biotechnol. 11:263-268.
  10. Laviolette FA, Athow KL, Probst AH, Wilcox JR. 1970. Effect of bacterial pustule on yield of soybeans. Crop Sci. 10:150-151. https://doi.org/10.2135/cropsci1970.0011183X001000020008x
  11. Lee S. 1999. Occurrence and characterization of major plant bacterial diseases in Korea. Dissertation, Seoul National University.
  12. Moon JK, Seo MJ, Ku JH. 2005. Screening of resistant germplasm and development of molecular markers against soybean bacterial pustule disease. Treat. of Crop Sci. 6:149-160.
  13. Narvel JM, Jakkula LR, Phillips DV, Wang T, Lee SH, Boerma HR. 2001. Molecular mapping of Rxp conditioning reaction to bacterial pustule in soybean. J. Hered. 92:267-270. https://doi.org/10.1093/jhered/92.3.267
  14. Palmer RG, Lim SM, Hedges BR. 1992. Testing for linkage between the Rxp locus and nine isozyme loci in soybean. Crop Sci. 32:681-683. https://doi.org/10.2135/cropsci1992.0011183X003200030020x
  15. Seo MJ, Kang ST, Moon JK, Lee S, Kim YH, Jeong KH, Yun HT. 2009. Identification of Quantitative trait loci associated with resistance to bacterial pustule (Xanthomonas axonopodis pv. Glycines) in soybean. Korea J. Breed. Sci. 41(4): 456-462.
  16. Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB. 2004. A new integrated genetic linkage map of the soybean. Theor. Appl. Genet. 109:122-128. https://doi.org/10.1007/s00122-004-1602-3
  17. Van K, Ha BK, Kim MY, Moon JK, Paek NC, Heu S, Lee SH. 2004. SSR mapping of genes conditioning soybean resistance to six isolates of Xanthomonas axonopodis pv. glycines. Korean J. Genet. 26:47-54.
  18. Van K, Kim DH, Cai CM, Kim MY, Shin JH, Graham MA, Shoemaker RC, Choi BS, Yang TJ, Lee SH. 2008. Sequence level analysis of recently duplicated regions in soybean [Glycine max (L.) Merr.] genome. DNA Res. 15:93-102. https://doi.org/10.1093/dnares/dsn001