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Development of Molecular Markers Conferring Bacterial Leaf Pustule Resistance Gene, rxp, using Resistant and Susceptible Cultivars in Soybean  

Yang, Kiwoung (Department of Functional Crop, NICS, RDA)
Lee, Yeong Hoon (Department of Functional Crop, NICS, RDA)
Ko, Jong Min (Department of Functional Crop, NICS, RDA)
Jeon, Myeong Gi (Department of Functional Crop, NICS, RDA)
Lee, Byong Won (Department of Functional Crop, NICS, RDA)
Kim, Hyun Tae (Department of Functional Crop, NICS, RDA)
Yun, Hong Tae (Department of Functional Crop, NICS, RDA)
Jung, Chan Sik (Department of Functional Crop, NICS, RDA)
Baek, In Youl (Department of Functional Crop, NICS, RDA)
Publication Information
Korean Journal of Breeding Science / v.43, no.4, 2011 , pp. 282-287 More about this Journal
Abstract
Bacterial pustule (BP) is a leaf disease of soybean that is most common in Korea. Inoculation of 8ra, pathogen strain, to resistant and susceptible cultivars for finding the BP resistance gene (rxp) was much tried but the sequence of the exact gene is not found. This research performed in order to confirm the rxp gene near molecular marker by using the resistant and susceptible cultivars. Soybean BP resistance gene which related to region of near molecular marker could select the resistant cultivar. For the near molecular marker of rxp, reference genomics data available at sequenced Phytozome was used for designing molecular markers. The rxp was mapped between Satt372 and Satt486 on chromosome 17. According to previous study, rxp released in find mapping 7.2 Mbp to 7.3 Mbp on chromosome 17. In this study, we developed 3 random markers near from 6.6 Mbp to 7.3 Mbp on chromosome 17 identified to increase the genetic resolution of the rxp gene region using resistant and susceptible cultivars. Particularly, Rxp17-700 marker was mostly coincided resistance and susceptible genotype to rxp. This result suggests that Rxp17-700 marker will be more tightly linked to rxp gene.
Keywords
Bacterial pustule(BP); BP resistance gene(rxp); Near molecular markers; Soybean;
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