• Journal of fish pathology
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• v.24 no.2
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• pp.53-64
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• 2011

In quantitative studies of clinical signs, rock bream of adults and juveniles infected with Megalocytivirus IVS-1 isolated from rock bream (Oplegnathus fasciatus) in Korea showed average $4.49{\pm}1.13$ and $4.85{\pm}1.06$ of spleen index respectively. In challenge experiments, Megalocytivirus IVS-1 induced 100% cumulative mortality in both adult and juvenile rock bream. However we found 60% cumulative mortality in juvenile red sea bream (Pagrus major) even after 30 days of injection, which contradicted with the results of other laboratories. Interestingly, IVS-1 infected red sea bream of the same juvenile size with rock bream showed lower spleen index compared to that of rock bream. In real-time PCR, there was continuous increasing of the numbers of viral copies ($2.03{\times}10^7$ copies/mg) in the spleen of juvenile rock bream infected, which were different from those in adult rock bream showing plateau level after reaching to the peak level. Moreover, enlarged cell numbers in the infected spleen were also increased continuously in the juvenile but not in adult of rock bream, even decreased after reaching to peak level. Consequently, significant differences in clinical signs: cumulative mortality. spleen index and viral copy number were found between rock bream and red sea bream, but not between adult and juvenile rock bream. Certainly quantitative expression of clinical sign as in this study may be a way to compare the progression of megalocitiviral disease more accurately in different species or physiological conditions.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.29 no.1
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• pp.11-19
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• 2005

Traditionally Jeju island has a mild oceanic climate throughout the year and famous as worldwide resort area bacause of its pure natural environment and dramatic coastal scenery. But unpredicted coastal erosion problem, caused by variation of environmental conditions from construction of coastal structure and renovation of the existing ports, has raised its head above the water, and is becoming serious these days just like other coastal area in Korea. The phenomena happen here along the seaside of southern part of the island show that severe changes in coastal line from erosion and even witnessed the coastal cliff failure. In advanced countries, coastal engineers and researchers have studied deeply about this kind of problem for a long time. However, as it is not sot active in Korea and lack of research data, there exists difficulties on building protection methods and thoughtless constructions might make it more complicated and fatal to the coastal environment. In this study, we investigated some case studies of other countries and intended to induce and propose some integral protection methods for coastline erosion, considering environmentally sound and water friendly way of developement such as artificial reef, floating breakwater, and double cellblock breakwater. Finally, we made analysis on the proposed methods with numerical model test and evaluation on the feasibility of each method.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.5
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• pp.1-7
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• 2015

Recently, a coaching helps individual to lead and maximize performance. Generally there is a face to face coaching at the current spot of education, and the e-coaching is on the rise. And the face-to-face coaching means that coach and the coachee come face to face directly. And, the effective coaching method is required to supply the weakness and to maximize the strength of the e-coaching using the Internet. Accordingly, we are studying a hybrid coaching to overcome the difficulty and the limit of face-to-face coaching and online coaching. In this paper, we design and implement the career coaching system to provide the face-to-face coaching and the e-coaching for coachee based on the Web service on the Internet. We provide the hybrid coaching using Web service based on the Restful and connect with the database based on the Web to save and manage the profiles and the coaching history of the coach and the coachee. Proposed hybrid coaching system for coachee career improves remote accessibility between the coach and the coachee. This system provides the interaction without the limit of the time and the space, and overcomes the limit of the traditional face-to-face coaching on the online environment.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.335-345
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• 2020

Tomato grey mould has been one of the destructive fungal diseases during tomato production. Ten mM of menadione sodium bisulfite (MSB) was applied to tomato plants for eco-friendly control of the grey mould. MSB-reduced tomato grey mould in the 3rd true leaves was prolonged at least 7 days prior to the fungal inoculation of two inoculum densities (2 × 10⁴ and 2 × 10⁵ conidia/ml) of Botrytis cinerea. Protection efficacy was significantly higher in the leaves inoculated with the lower disease pressure of conidial suspension compared to the higher one. MSB-pretreatment was not effective to arrest oxalic acid-triggered necrosis on tomato leaves. Plant cell death and hydrogen peroxide accumulation were restricted in necrotic lesions of the B. cinereainoculated leaves by the MSB-pretreatment. Decreased conidia number and germ-tube elongation of B. cinerea were found at 10 h, and mycelial growth was also impeded at 24 h on the MSB-pretreated leaves. MSB-mediated disease suppressions were found in cotyledons and different positions (1st to 5th) of true leaves inoculated with the lower conidial suspension, but only 1st to 3rd true leaves showed decreases in lesion sizes by the higher inoculum density. Increasing MSB-pretreatment times more efficiently decreased the lesion size by the higher disease pressure. MSB led to inducible expressions of defence-related genes SlPR1a, SlPR1b, SlPIN2, SlACO1, SlChi3, and SlChi9 in tomato leaves prior to B. cinerea infection. These results suggest that MSB pretreatment can be a promising alternative to chemical fungicides for environment-friendly management of tomato grey mould.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.386-391
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• 2005

This study was done to produce a mutated protein inactivated cytotoxicity of Shigatoxin 2e (Stx2e) of E.coli O139 isolates by deletional mutagenesis of Stx2e A subunit gene encoding active-site cleft of enzymatic domain in ST2e holotoxin. Cytotoxicity of the toxoid expressed from the mutant Stx2e gene was compared with wild type Stx2e for development of vaccine candidate. A recombinant plasmid pED18 containing Stx2e gene ot E.coli O139 isolates was used to generate mutation plasmid. Deletion mutagenesis was conducted for Stx2e A subunit gene encoding enzymatically active domain by polymerase chain reaction (PCR) using ot designed primer to induce deletional mutation. DNA sequence analysis was confirmed that the pentamer (Typ 202- Ser 206) that lies within the proposed active-site cleft in the second region was completely deleted. A DNA fragment of 1.1 kb that encode the new mutant Stx2eA gene was inserted into plasmid pRSET vector digested with EcoRV-Hind III and named pEDSET The PEDSET was transformed in E. coli for expression of mutant protein and the protein was confirmed by SDS-PACE and Western-blotting. The protein expressed by the mutant was tested to confirm the reduction of cytotoxic activities on Vero cell using microcytotoxicity assay compared with wild type Stx2e, the cytotoxicity of deletional mutant protein was at least reduced by 3,000-fold on Vero cell.

• Journal of Technologic Dentistry
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• v.33 no.4
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• pp.413-425
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• 2011

Purpose: This study conducted a survey on the elderly with physical debilities, who are in a medically vulnerable social class, to examine closely their demo-sociological characteristics, unmet needs, dental states, and living qualities and satisfaction levels relating to oral health and social supports to them, and also to prepare the basis for effective public medical policies and health improvement programs aimed at improving the quality of life for the elderly with physical debilities. Methods: Twenty-two elderly care facilities within Jeju Special Self-Government Province participated in the survey. Between 11 January and 5 March 2010, a total of 250 elderly persons(65 and over) with physical debilities were interviewed and their dental health was checked. Results: The results of the survey are as follows. The need for social support for dental care of the elderly with physical debilities was high in the medical institution-supported service (49.6%). The unmet needs for physical care were high in bathing (49.6%) and using public transportation (71.6%). More than half of these surveyed had ten or fewer teeth. The survey found that 31.6% of the participants experienced problems eating, due to poor dental health. Concerning quality of life, 30.5% of those surveyed experienced physical pain. Conclusion: In summary, the ages of the survey participants directly relates to the degree of behavioral debility experienced. The more debility a participant exhibits, the greater is the need for social support and dental care. The dental health of a participant directly relates to a higher quality of life. Good dental health of a participant translate to better quality of life. In light of the fact that the elderly with physical debilities suffer from a lack of accessibility to medical care and worse oral health than do other elderly persons, it is essential to increase accessibility to medical institutions that can provide such services as door to door dental care. Current insurance policies, funding for denture insurance, and free denture and denture-upgrade programs desperately need to be expanded. Therefore, to improve effectively the quality of life for the elderly with physical debilities civil dental medical resources should be encouraged to provide inclusive and prevention-focused medical care. In the public domain, door to door dental care services and cooperation with civil dental care resources need to be improved to increase impartial accessibility to dental medical institutions.

• Journal of the Korean Geographical Society
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• v.43 no.4
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• pp.477-494
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• 2008

Stable isotope analyses on lake or wetland sediments are useful to reconstruct paleoclimate. Organic and inorganic carbonates obtained from lake sediment are isotopically analyzed to get oxygen and carbon isotopic ratios. Oxygen isotope ratios can be used to quantitatively and qualitatively reconstruct paleo-temperature or humidity while carbon isotope ratios be used to reveal environmental changes around the lake or human impacts on the area. Peat mosses in peat bogs are nice samples for the carbon isotope analysis, which derives paleo-temperature and paleo-atmospheric $CO_2$ changes. In coastal area, the reconstruction of past sea-level is possible because terrestrial originated organic matter is carbon isotopically different from marine originated organic matter. Also, scientists can do research on Asian Monsoon based on the fact that $\delta^{13}C$ of C3 plants and C4 plants are consistently different each other and that they are distributed differently with respect to salinity. In Korea, paleoenvironmental studies using stable isotopes are not popular yet because of low academic interests on the methodology and difficulties of obtaining proper sediment samples. Interesting results can be produced to answer paleoenvironmental questions of Korea if scientists isotopically analyze sediment cores from a paleo-lake such as Hanon in Jeju island, peat bogs such as Mujechi-Neup and Yong-Neup, and coastal wetlands.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.1
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• pp.247-257
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• 2020

Despite the various smoking cessation programs that are available for college students, students have lower rates of quitting smoking than do other age groups. This study identifies the variables associated with continuous abstinence from smoking among college students. This study used the data from the National Tobacco Control Center and 781 college students who participated in the program conducted by the Daejeon Tobacco Control Center from June, 2015 to December, 2016. The results showed that the expiration CO level and the frequency of attending smoking cessation counseling were the significant variables related to the continuous abstinence rate at 4, 12, and 24 weeks. Students who had a low expiration CO level (?10 ppm) had a higher abstinence rate than did the students who had a high expiration CO level (≥10ppm), and the OR was 2.53 at 4-week, 2.33 at 12-week, and 2.13 at 24-week. The ORs for the 4-week, 12-week, and 24-week abstinence rates with one additional counseling session were 12.39, 13.13, and 12.21, respectively. This study suggests the need to increase the number of smoking cessation counseling sessions for effective smoking cessation intervention among college students.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.470-470
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• 2011

환경부 하수재이용사업은 2007년도 하수도법 개정을 통해 공공하수처리시설 처리수의 재이용 의무화를 시작으로 2010년도 "물의 재이용 촉진 및 지원에 관한 법률"의 시행으로 전국적으로 사업이 확대되고 있다. 제주도 월정하수처리장은 2009년도 하수재이용사업지구로 선정이 되어 2010년도부터 농업용목적의 재이용으로 구체적인 설계와 시공이 추진되고 있는 사업지구이다. 제주도에서는 지하수보존을 위해 대체수자원 개발 방안을 시급히 마련중에 있다. 특히, 제주도 농업용수 종합계획수립(제주도, 2004)에는 하수처리수를 농업용수로 이용하기 위한 계획을 수립할 정도로 지하수 사용량을 줄이기 위한 노력을 진행중에 있으며, 그 일환으로 하수재이용사업을 지속적으로 추진하고 있다. 하수처리수의 농업용수 재이용은 사용된 물을 재이용함으로써 물과 에너지를 절약할 수 있고, 유역 또는 해양으로 배출되는 오염원을 억제하는 장점이 있는 반면에 농산물 생육에 직접 영향을 줄뿐만 아니라 주변의 수질 생태 토양 환경 및 영농인의 보건에도 영향을 주기 때문에 장기적인 관찰과 검증작업이 필요하다. 이 에 서울대와 한국농어촌공사는 안전한 농업용수 재이용기술을 개발하기 위해 장기간 현장시험을 통해 재이용 재배기술과 함께 보건환경에 미치는 영향을 검증하였고 그 개발기술을 월정사업지구에 적용하게 되었다. 월정하수처리장이 위치한 제주 동부의 월정지역은 농지면적이 밭(374ha)과 과수(12ha)등 제주도의 전형적인 농촌마을으로 주요 재배작물은 마늘과 당근, 쪽파, 콩 등으로서 농업기반시설의 미비로 영농에 어려움을 호소하고 있으며, 2006년도에 발생한 가뭄으로 그 해 평균 수학량의 30%가 감소된 바 있는 지역이다. 제주도 농업용수 종합계획수립(2004, 제주도)에서는 10년에 한발을 기준 으로 $43,000m^3$/일의 용수가 부족할 것으로 분석하였으며 최근 $35,000m^3$/day 규모의 상수도 확보사업 계획 수립하였으나 여전히 농업용수가 부족하다. 방류수의 수질은 방류수수질기준을 만족하지만 염분함량이 높아 직접 농업용으로 사용하기에는 적당하기 않고, 농업용재이용방류수 수질기준에 맞도록 재이용시스템을 통해 재처리하여 농업용수로 사용해야 한다. 제주도에서는 이미 제주 서부하수처리장 농업용수 재이용사업(이하 판포재이용사업)'이 완료되어 재이용수를 농업용수로 공급하고 있으며 향후 지속적으로 하수재이용사업이 확대될 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.555-562
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• 2012

PCR was performed to analyze the ${\beta}$-lactamase genes carried by ampicillin-resistant Vibrio spp. strains isolated from marine environments in Korea between 2006 and 2009. All 36 strains tested showed negative results in PCR with the primers designed from the nucleotide sequences of various known ${\beta}$-lactamase genes. This prompted us to screen new ${\beta}$-lactamase genes. A novel ${\beta}$-lactamase gene was cloned from Vibrio alginolyticus KV3 isolated from the aquaculture water of Geoje Island of Korea. The determined nucleotide sequence (VAK-3 ${\beta}$-lactamase) revealed an open reading frame (ORF) of 852 bp, encoding a protein of 283 amino acids (aa), which displayed low homology to any other ${\beta}$-lactamase genes reported in public databases. The deduced 283 aa sequence of VAK-3, consisting of a 19 aa signal peptide and a 264 aa mature protein, contained highly conserved peptide segments specific to class A ${\beta}$-lactamases including the specific amino acid residues STFK (62-65), SDN (122-124), E (158), and RTG (226-228). Results from PCR performed with primers specific to the VAK-3 ${\beta}$-lactamase gene identified 3 of the 36 isolated strains as V. alginolyticus, Vibrio cholerae, and Photobacterium damselae subsp. damselae, indicating the utilization of various ${\beta}$-lactamase genes including unidentified ones in ampicillin-resistant Vibrio spp. strains from the marine environment. In a mating experiment, none of the isolates transfered the VAK-3 ${\beta}$-lactamase gene to the Escherichia coli recipient. This lack of mobility, and the presence of a chromosomal acyl-CoA flanking sequence upstream of the VAK-3 ${\beta}$-lactamase gene, led to the assumption that the location of this new ${\beta}$-lactamase gene was in the chromosome, rather than the mobile plasmid. Antibiotic susceptibility of VAK-3 ${\beta}$-lactamase was indicated by elevated levels of resistance to penicillins, but not to cephalosporins in the wild type and E. coli harboring recombinant plasmid pKV-3, compared with those of the host strain alone. Phylogenetic analysis showed that VAK-3 ${\beta}$-lactamase is a new and separate member of class A ${\beta}$-lactamases.