한국어병학회지 (Journal of fish pathology)

  • 제24권2호
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  • Pages.53-64
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  • 2011
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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Megalocytivirus 감염 해산 어류에서 나타나는 임상증상의 정량적 변화 분석

Quantitative analysis of the clinical signs in marine fish induced by Megalocytivirus infection

  • 진지웅 (부경대학교 수산생명의학과) ;
  • 조혜진 (수산자원연구소 민물고기 연구센터) ;
  • 김광일 (부경대학교 수산생명의학과) ;
  • 정준범 (제주대학교 해양의생명과학부) ;
  • 박경현 (수자원사업단) ;
  • 정현도 (부경대학교 수산생명의학과)
  • Jin, Ji-Woong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Cho, Hye-Jin (Fisheries Resources Institute) ;
  • Kim, Kwang-Il (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong, Joon-Bum (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University) ;
  • Park, Gyeong-Hyun (Fisheries Resources Agency) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2011.04.06
  • 심사 : 2011.07.14
  • 발행 : 2011.08.31
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초록

Megalocytivirus 감염 시 나타나는 임상적 증상의 정량적인 분석을 위하여, 돌돔에서 분리된 megalocytivirus IVS-1을 돌돔성어와 치어에 인위 감염시켰다. 그 결과 spleen index가 각각 $4.49{\pm}1.13$와 $4.85{\pm}1.06$로 나타 났으며, 이것은 정상배에 비해 3배 이상 증가한 값이었다. 폐사율은 돌돔성어와 치어에서 모두 100% 폐사한 반면 참돔치어는 30일이 지나도 60%의 폐사만 나타나 돌돔폐사율에 비해 낮은 폐사율을 나타냈다. 또한 IVS-1을 감염시킨 빈사상태의 참돔치어는 spleen index가 동일 크기의 돌돔치어 보다 낮은 $1.47{\pm}0.87$을 보였다. Real-time PCR을 이용하여 감염의 진행에 따른 바이러스 농도를 측정한 결과, 돌돔 치어와 성어의 감염 조직 내 바이러스 최고 농도는 각각 $2.03{\times}10^7$ copies/mg과 $2.40{\times}10^7$ copies/mg으로 비슷하였으나 돌돔성어에서 먼저 최고치에 도달하였다. 더구나 비장의 비정형비대세포의 수 역시 돌돔치어의 경우 지속적으로 증가한 반면 돌돔성어의 경우 최고치에 도달 후 오히려 감소히는 경향을 보였다. 본 연구에서는 megalocytivirus 감염 시 조직의 형태적 변화와 조직 내의 바이러스 농도와 같은 임상적인 증상을 정량적인 분석을 통해 나타내었고 이러한 정량적인 수치와 megalocytivirus 감염의 진행 정도와의 상관관계에 대해 조사하였다.

In quantitative studies of clinical signs, rock bream of adults and juveniles infected with Megalocytivirus IVS-1 isolated from rock bream (Oplegnathus fasciatus) in Korea showed average $4.49{\pm}1.13$ and $4.85{\pm}1.06$ of spleen index respectively. In challenge experiments, Megalocytivirus IVS-1 induced 100% cumulative mortality in both adult and juvenile rock bream. However we found 60% cumulative mortality in juvenile red sea bream (Pagrus major) even after 30 days of injection, which contradicted with the results of other laboratories. Interestingly, IVS-1 infected red sea bream of the same juvenile size with rock bream showed lower spleen index compared to that of rock bream. In real-time PCR, there was continuous increasing of the numbers of viral copies ($2.03{\times}10^7$ copies/mg) in the spleen of juvenile rock bream infected, which were different from those in adult rock bream showing plateau level after reaching to the peak level. Moreover, enlarged cell numbers in the infected spleen were also increased continuously in the juvenile but not in adult of rock bream, even decreased after reaching to peak level. Consequently, significant differences in clinical signs: cumulative mortality. spleen index and viral copy number were found between rock bream and red sea bream, but not between adult and juvenile rock bream. Certainly quantitative expression of clinical sign as in this study may be a way to compare the progression of megalocitiviral disease more accurately in different species or physiological conditions.

키워드

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피인용 문헌

  1. Identification and Characterization of Megalocytivirus Type 3 Infection with Low Mortality in Starry Flounder, Platichthys stellatus , in Korea vol.49, pp.1, 2018, https://doi.org/10.1111/jwas.12439
  2. vol.41, pp.8, 2018, https://doi.org/10.1111/jfd.12815
  3. Correlation of virus replication and spleen index in rock bream iridovirus infected rock bream Oplegnathus fasciatus vol.32, pp.1, 2019, https://doi.org/10.7847/jfp.2019.32.1.001
  4. Co-Expression Network Analysis of Spleen Transcriptome in Rock Bream ( Oplegnathus fasciatus ) Naturally Infected with Rock Bream Iridovirus (RBIV) vol.21, pp.5, 2011, https://doi.org/10.3390/ijms21051707