• Korean Journal of Medicinal Crop Science
• /
• v.15 no.3
• /
• pp.199-202
• /
• 2007

This study was carried out to provide pest control information for the cultivation of Eleutherococcus senticosus Max. As a result of investigation of the diseases and insect pests, three pathogens and three insect pests were identified from the field sample, respectively. The identified insects pests were aphids, stinkbugs and Bothrogonia japonica. Occurrenre peaks of aphids were occurred on June and August, and generally young leaves and flower buds were injured mainly. The identified diseases were black ring spot caused by Phoma sp., gray mold caused by Botrytis cinerea and leaf blights caused by Rhizoctonia solani. The black ring spot was the most severe disease and was investigated from the beginning of June, and the incidence of the disease was 30% higher than that on September. As a result of growth test under different temperature conditions on PDA, these pathogens showed the best mycelial growth rate between 25 $^{\circ}$C and 30 $^{\circ}$C, and these results indicate that these diseases occur on hat summer season.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.10
• /
• pp.1223-1229
• /
• 2009

To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans), and wheat leaf rust (Puccinia recondita), by electroporation. The recombinant B. velezensis strain was confirmed by PCR using cry1Ac-specific primers. Additionally, the recombinant strain produced a protein approximately 130 kDa in size and parasporal inclusion bodies similar to B. thuringiensis. The in vivo antifungal activity assay demonstrated that the activity of the recombinant B. velezensis strain was maintained at the same level as that of wild-type B. velezensis. Furthermore, it exhibited high insecticidal activity against a lepidopteran pest, Plutella xylostella, although its activity was lower than that of a recombinant B. thuringiensis strain, whereas wild-type B. velezensis strain did not show any insecticidal activity. These results suggest that this recombinant B. velezensis strain can be used to control harmful insect pests and fungal diseases simultaneously in one crop.

• Korean journal of applied entomology
• /
• v.57 no.2
• /
• pp.105-115
• /
• 2018

The comstock mealybug is one of the serious insect pest in pear. It is necessary effective control methods such as attractive sex pheromone. Using sex pheromones is necessary for studying the physiology and ecology of insect pests. In the present study, the sex pheromone of comstock mealybug was used for investigating the flying acts of the adult male. As temperature increased, the development period of eggs shortened, but it was longer at $30^{\circ}C$. The mean number of eggs laid was 482 at $25^{\circ}C$ and males constituted 37% of the experimental population at $30^{\circ}C$. The flight of the male comstock mealybug was mostly restricted to a 2-4 hour period after sunrise, even if condition of light period is changed. Male mealybugs were incapable of upwind flight towards an attractant when air velocity was 1.5 mph. Preference of height was tested in a pear orchard. The ineffective heights of the traps were 2 and 2.5 m, whereas the effective heights were 1 and 1.5 m. Many captured males at 0.5, 1, and 5 m, and the number of captured males were similar in 10, 15, and 20 m. Male mealybugs were captured up to a distance of 50 m, suggesting that it is possible to control the male mealybugs that are more than 50 m away when sex pheromone traps are used.

• Asian Journal of Turfgrass Science
• /
• v.19 no.1
• /
• pp.39-46
• /
• 2005

The 21 strains of Korean entomopathogenic nematodes, {Heterorhabditis bacteriophora Hamyang strain(HbH), Heterorhabditis sp. 202, 205, 217, Heterorhabdiris sp. KCTC 0991BP strain, Steinernema carpocapsae Pocheon(ScP), S. longicaudum Gonaju, S. longicaudum Nonsan, Steinernema sp. 7,24, 52, 55, 60, 64, 206, 207, 209, 210, 219, and 227 strain} were evaluated for the control of a turfgrass insect pest, Exomala orientalis. Heterorhabditis spp. showed higher pathogenicity than Steinernema spp. against 3rd instar larvae of E. orientalis with $55\%$ mortality by Heterorhabditis sp. 202 strain and $50\%$ by HbH and Heterorhabditis sp.205 strain at the rate of 200 infective juveniles per larva 14 days later after treatment. The number of infective juveniles of Korean entomopathogenic nematodes in 3rd instar larvae of E. orientalis was higher in Heterorhabditis spp. than in Steinernema spp.. In general, numbers of produced infective juveniles of three species were much higher, i.e., Heterorhabditis sp.202 strain produced 273,064 infective juveniles, S. carpocapsae Pocheon strain 273,043, and Heterorhabditis sp. 217 strain 248,887, respectively.

• Korean journal of applied entomology
• /
• v.45 no.3 s.144
• /
• pp.357-362
• /
• 2006

Bacillus thuringiensis strains were isolated from the domestic soil and a strain was selected that had a new host range and high toxicity against agriculture insect pest. The 142 samples of soil were sampled from the mountains, paddy fields and patches, in Daejon, Chungnam, Chungbuk and Jeonbuk and used for the investigation. Sixteen B. t strains were isolated from 12 samples among collected samples. There were 11 strains that showed toxical activity on Plutella xylostella (Lepidoptera: Yponomeutidae), 7 steins on Spodoptera litura (Lepidoptera: Noctuidae), 5 strains on Arete coerulea (Lepidoptera: Noctuidae), 5 strains on Culex pipiens pallens (Diptera: Culicidae) among the 16 isolated B. t strains. But there were not any strains that showed activity against Hyphanria cunea (Lepidoptera: Arctiidae) and Sitophilus oryzae (Coleoptera: Rhynchophoridae). And also some of B. thuringiensis strains showed insecticidal activity with 2, 3 or 4 kinds of insects. But there were also 3 strains that did not show any activities to the 6 insects which were used in the experiment. When examined with a phase-contrast microscope, the insecticidal crystal protein produced from 16 selected strains had 13 bipyramidal and 3 spherical shapes. The insecticidal bioactivity of the S. litura showed 100% mortality when there were $1.3{\times}10^{7}\;(cfu/ml)$ of CAB109 isolates.

• Korean journal of applied entomology
• /
• v.44 no.2
• /
• pp.145-150
• /
• 2005

Three Korean isolates of entomopathogenic nematodes, Steinernema carpocapsae KCTC 0981BP (ScK), S. glaseri Dongrae (SgD), and Heterorhabditis sp. KCTC 0991BP (HsK), were evaluated for the control of a turfgrass pest, Popillia quadriguttata. Three days after treatment, all nematodes showed high pathogenicity to adult P. quadriguttata with $97.5\%$ mortality by ScK, $90.8\%$ by HsK, and $80\%$ by SgD at the concentration of 900 infective juveniles per adult. Nematode attachment and infection rate to adult P. quadriguttata were various depending on nematode species and inoculation density. The rate of nematode attachment was $90.8\%$ in HsK, $90.6\%$ in SgD, and $35\%$ in ScK, resfectively at the concentration of 900 infective juveniles per adult. The infection rate that represents the rate of detected nematode from inside insect of body was $97.5\%$ in ScK, $ 80\%$ in both HsK and SgD at the 900 concentration of infected juveniles. The infection rate, however, was decreased to $27.5\%$ in ScK, $72.5\%$ in SgD, but no nematodes was detected in HsK at the concentration of 90 infective juveniles.

• Korean journal of applied entomology
• /
• v.2
• /
• pp.47-54
• /
• 1963

The chestnut gall wasp, Dryocosmus kuriphilus $Y^{ASUMATSU}$, has been recently distributed and chestnut trees have teen seriously damaged by this insects in Korea. However, this insect pest has not been investigated in Korea. This paper gives an account of field and Laboratory observations on the seasonal occurrence and the life history of the chestnut gall wasp, and the damage of the chestnut trees by. its insects. This study was carried out at Chungchungpukdo, Kangwon-do and Kyungi-do in Korea, from 1961 to 1963, inclusive. 1. The Chestnut gall wasp (Dryocosmus kuriphilus $Y^{ASUMATSU}$) has one generathion per year and females are only known. 2. The adult wasps were observed from late June to late July with peak of wasp emergence about early and middle July. Most of new adult wasps were coming out from, the galls in the morning rather than the afternoon. 3. Each wasp produced auerage 198.5 eggs and about 4.89 eggs was observed in each bud of the chestnut tree. 4, The egg period of wasps was about 30 days and they were hatching from July to August. 5. The larval period of wasps were from middle of August to middle of next June and pupation took places about early June to middle of July. 6. The adult wasps were ovipositing into the buds and from early May next year, galls were growing. They caused stunting of growth and no fruiting or dying of the chestnut tries. 7. The first observation of the wasp was made at Chaechun of Chungchung-pukdo in 1958. Up to 1963 this insects are distributed in Kangwon-do, Kyunggi-do, north part of Chungchung-pukdo and north part of Kyungsangpukdo

• Korean journal of applied entomology
• /
• v.17 no.1 s.34
• /
• pp.41-47
• /
• 1978

Pheromones of several insect species were evaluated in a screening program in terms of their usefulness in pest forecasting and control in Korea. Species included striped rite borer (Chilo suppressalis) and tortricid moths, which attack deciduous fruit, and colding moth. The pheromone of striped rice borer was supplied through the courtesy of the Tropical Products Institute, London. Pheromones of other species were obtained from Cornell University and the Zoecon Corporation of Palo Alto, California. 'rho results of this experiment were as follows: 1. Live traps containing virgin striped rice borer female moths were more effective in attracting male moths than were the pheromone traps. 2. Since the effectiveness of the striped rice borer pheromone decreased dramatically with time, it was difficult to estimate the peak tine of the borer emergence. 3. The primary species trapped in deciduous fruit orchards was the oriental fruit moth, Grapholitha molesta. The traps were baited with the phermones, OFM and LAW. 4. Several moth species were trapped with OBLR. RBLR. SPAR and ArcM phermones but few were trapped with the remaining eight tortricid pheromones. 5. The following tortricid pheromones might be useful for forecasting the species given: OFM, LAW : Grapholitha molesta OBLR : Archips breviprecanus OBLH, RBLR, ArcM : Archippus coreensis Archips fuscocupreanus Hoshinoa longicellana SPAR, TBM ; Phyroderces sp.

• Journal of Bio-Environment Control
• /
• v.28 no.2
• /
• pp.150-157
• /
• 2019

This study was carried out to investigate at the micrometeorology change of the orchard according to the net installation. Two weather stations were installed at the inside of the anti-insect nets(2 mm, 4 mm), one was installed at the outside(control). They were observed the temperature, humidity, wind speed and solar radiation from April to September 2018. Daily mean temperature at the experimental group was higher than control group by $0.2^{\circ}C$. Daily mean humidity at the experimental group was higher than control group by 3.5 to 4.7%. Daily mean the solar radiation at the experimental group(2 mm) was lower than control group by 50%. The wind speed was decreased from 12% to 50% of the external wind speed at 4 mm, and from 25% to 59% at 2 mm.

• Korean journal of applied entomology
• /
• v.62 no.3
• /
• pp.161-169
• /
• 2023

Thrips are usually not exposed to insecticide spray due to hiding at holes, gaps, or crevices of host plants with their relatively small body sizes. This study devised a strategy to use a contact pheromone to suppress the seclusive behavior of the thrips, A contact pheromone identified as 7-methyltricosane (7TM) in the western flower thrips, Frankliniella occidentalis, was added to the diet to test the preference of the thrips. Although 7TM did not change the larval behavior, it significantly induced the avoiding behavior in male adults. In contrast, the contact pheromone was preferred by the female adults. Similar behavioral changes were also observed in another flower thrips, Frankliniella intonsa. Based on the behavioral changes induced by the contact pheromone, a mixture of 7TM and insecticide was applied to thrips infesting hot peppers in field conditions. Compared to spinetoram treatment, its mixture treatment with 7TM enhanced the control efficacy against thrips. Interestingly, 7TM treatment alone also gave slight reduction in F. intonsa density, suggesting a behavioral disruption of thrips by the contact pheromone. This study suggests a novel technology to control insect pests using contact pheromone by suppressing the seclusive behavior to avoid exposure to insecticide spray.