• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.2 s.115
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• pp.80-98
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• 2006

This study originated from following questions. What can we understand the conception of deconstruction, which has been the core idea of new discourses developed in various ways since modernism? How can this question be interpreted in landscape design? What is the conceptional frame of integration the prominent hybrid post-genre movements and phenomena? The frame can be epitomized with the deconstruction phenomenon. 'Deconstruction' is the core conception appeared in late or post-modern ages in the embodiment of modernity and can be viewed as an integrating or a hybrid phenomenon between areas or genres in formative arts. Therefore, the author regards the hybrid movements widely witnessed in the post contemporary formative arts as one of the most important indicators of de-constructive signs. It is safe to say that the phenomenon of this integration or hybridism, of course, does not threaten the identity of landscape design but serves as an opportunity to extend the areas of landscape design. One of the consequences of this integration or hybridism is the voluntary participation of users who have been alienated in the production of the meanings of design works and hybrid landscape design with the hybridization of genres that is characterized with transformation in forms. This view is based on the distinction between hybridization of interactions between the designer (the subject) and the user (the object), and hybridization of synesthesia. Generally speaking, this is an act of destroying boundaries of the daily life and arts. At the same time, it corresponds to vanishing of modern aesthetics and emerging of post-contemporary aesthetics which is a new aesthetic category like sublimeness. This types of landscape design tries to restore humans' sensibility and perceptions restrained by rationality and recognition in previous approach and to express non-materialistic characteristics with precaution against excessive materialism in the modern era. In light of these backgrounds, the study aims to suggest the hybrid concept and to explorer a new landscape design approach with this concept, in order to change the design structure from 'completed' or 'closed' toward 'opened' and to understand the characteristics of interactions between users and designs. This new approach is expected to create an open-space integrating complexity and dynamics of users. At the same time, it emphasizes senses of user' body with synesthesia and non-determination. The focus is placed on user participation and sublimity rather than on aesthetic beauty, which kind of experience is called simulacre. By attaching importance to user participation, the work got free from the material characteristics, and acceptance from the old practice of simple perception and contemplation. The boundaries between the subject and object and the beautiful and ordinary, from the perspective of this approach, are vanished. Now everything ordinary can become an artistic work. Western dichotomy and discrimination is not effective any more. And there is 'de-construction' where there is perfect equality between ordinary daily life and beautiful arts. Thus today's landscape design pays attention to the user and uses newly perceived sensitivity by pursing obscure and unfamiliar things rather than aesthetic beauty. Space is accordingly defined to take place accidentally as happening and event, not as volume of shape. It's the true way to express spatiality of landscape design. That's an attempt to reject conventional concepts about forms and space, which served as the basis for landscape design, and to search for new things.

• 대한공업교육학회지
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• v.38 no.1
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• pp.29-48
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• 2013

The purpose of this research is to provide a desirable case of STEAM education utilizing 'Energy and Transportation Technology' unit on the subject of technology home economics. The middle school students learned with the integrated approach based on STEAM integrated education, and through production process they had the opportunity for application and expression in diverse forms. The results of this process to achieve the purpose are as follows. First, as the procedural model to develop STEAM program utilizing 'Energy and Transportation Technology' unit on the subject of technology home economics, the five stages: preparation, development, verification, practice, and evaluation, were presented. The preparation stage was composed of requirement analysis, the selection of program subject, and STEAM program curriculum analysis, the selection and organization of STEAM program lesson. The development stage was composed of the development of lesson plan, multimedia teaching materials, worksheet and worksheet answer key. In the verification stage, the verification of validity by experts was conducted, and in the practice stage, the developed program was applied to the middle school students in the educational field, and in the evaluation stage, based on the evaluations received from learners and teachers, it was revised and supplemented. Second, the STEAM program was developed into the program summary map, lesson plan, multimedia teaching materials, worksheet and worksheet answer, etc., and after the validity was secured through experts' verification, it was revised and supplemented and applied to actual classes. Third, the results of the learners' evaluation of the developed STEAM program showed that the degree of satisfaction with the program was high with the average score of the entire questions being 4.00 on a five-point scale. As the teachers also evaluated the developed STEAM program as very effective, the opinions of learners and teachers were collected and the program was finally improved and completed.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.30 no.1
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• pp.1-9
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• 2020

Objectives: The purpose of this case study is to assess workers' exposure to carbon nanotubes(CNTs) and characterize particles aerosolized during the process of producing CNT-enabled polytetrafuoroethylene(PTFE) composites at a worksite in Korea. Methods: Personal breathing zone and area samples were collected for determining respirable concentrations of elemental carbon(EC) using NIOSH(National Institute for Occupational Safety and Health) Method 5040. Personal exposure to nano-sized particles was measured as the number concentration and mean diameter using personal ultrafine particle monitors. The number concentration by particle size was measured using optical particle sizers(OPS) and scanning mobility particle sizers(SMPS). Transmission electron microscopy (TEM) area samples were collected on TEM grids and analyzed to characterize the size, morphology, and chemistry of the particles. Results: Respirable EC concentrations ranged from 0.04 to 0.24 ㎍/㎥, which were below 23% of the exposure limit recommended by NIOSH and lower than background concentrations. Number concentrations by particle size measured using OPS and SMPS were not noticeably elevated during CNT-PTFE composite work. Instant increase of number concentrations of nano-sized particles was observed during manual sanding of CNT-PTFE composites. Both number concentrations and mean diameters did not show a statistically significant difference between workers handing CNT-added and not-added materials. TEM analyses revealed the emission of free-standing CNTs and CNT-PTFE aggregate particles from the powder supply task and composite particles embedded with CNTs from the computer numerical control(CNC) machining task with more than tens of micrometers in diameter. No free-standing CNT particles were observed from the CNC machining task. Conclusions: Significant worker exposure to respirable CNTs was not found, but the aerosolization of CNTs and CNT-embedded composite particles were observed during handing of CNT-PTFE powders and CNC machining of CNT-PTFE composites. Considering the limited knowledge on the toxicity of CNTs and CNT composite particles to date, it seems prudent to take a precautionary approach for the protection of workers' health.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.1
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• pp.1-6
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• 2004

Agricultural recycling of livestock wastes by composting has lots of beneficial effects on crop production and soil fertility. Most of composts are made from pig manure and water content controller such as saw dust, bark, rice hulls etc. by aerobic processing. But the insufficient supply of saw dust, bark etc. cause the indiscreet use of industrial waste having heavy metals and toxic synthetic chemicals. This research investigated the present quality status of organic by-product fertilizers, and suggested the way of quality interpretation under the statistical approaches based on median, mean and weighted average value. Since the data of one hundred of samples for heavy metal contents (Cd, Pb, Cu, and Cr) and OM/N showed extremely left-skewed distribution, the median was more useful than the mean in representing the characteristics of distribiition for each items. The weighted average value will be a useful index for the quality based on the total amount of producing.

• Cartoon and Animation Studies
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• s.14
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• pp.165-176
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• 2008

In the future there would be many kinds of digital images for many industrial markets. 3D stereoscopic images for variable fields; medical operation, film and animation, broadcasting, internet, game, or design for art and architecture. And many people to work about computer programming, and digital image making will concern about it more and more. However, these kinds works and studies are focused on the professional technical fields like 3D display or computer programming technology so far. To revitalize the market of a variable stereoscopic contents, there should build up the foundation for easy processing of the making stereoscopic images. This paper is based on stereoscopic making skills for anaglyph system. An anaglyph system has an old history about making stereoscopic images, and very simple method to produce the stereoscopic images. Particularly this study is focused on color compositing technique, and camera positioning on the compositing system. It will help optimization of the environments to create 3D motion graphic and animation contents.

• Microbiology and Biotechnology Letters
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• v.36 no.4
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• pp.260-267
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• 2008

The choice of expression vector is very important for industrial production of proteins. Therefore, the systematic mining of promoters over a wider range of genetic resource and/or host is required. We previously reported a novel bidirectional reporting system (pBGR) for the isolation of promoters from metagenome and screened useful promoters that functioned constitutively in E. coli under general culture conditions. Among them, three promoter sequences including each upstream region were amplified by PCR and used to construct new expression vectors. To facilitate subcloning, a multi-cloning site was incorporated into the downstream region of the revere primer sequence. At these sites, GFP, esterase and $\beta$-glucosidase were subcloned and analyzed the constitutive expression ability of new promoter in terms of protein solubility and expression level. As a result, these vectors expressed the proteins constitutively to a level of $2{\sim}3%$ of the total cell protein in soluble fraction (>80 %). This study suggested that excavation of metagenomic promoters for construction of expression vector in a certain strain could provide a way for the development of the expression systems.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.179-188
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• 2017

White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the $7^{th}$ day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.358-363
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• 2014

We was investigated parasporal inclusion proteins change to use industrial medium of new strain Bacillus thuringiensis CAB 565, CAB 566. To confirm medium's oxygen efficient consist of glucose and yeast extract, we was conducted oxygen transfer coefficients (KLa) of medium's concentration and impeller in 20 l-Jar fermentor. When to increase air flow rate and medium concentration, KLa rate is rise. Also it is effective on agitation rate 200 rpm, but KLa rate is decrease when to rise agitation rate. To hold dissolved oxygen rate (upper 50%), Air flow rate is steadily increase on culture to use microsparger. When 16 hour of culture stage, B.t. CAB 565 and B.t. CAB 566 harvested respectively $2.3{\times}10^{10}$, $1.8{\times}10^{10}$ viable cell/ml. When 54 hour, B.t. CAB565, 566 harvested respectively $1.9{\times}10^{10}$, $1.4{\times}10^{10}spore/ml$. To resulting carbon's concentration, It is the most effective that glucose concentration is contained 5% in medium.

• 대한공업교육학회지
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• v.36 no.2
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• pp.25-46
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• 2011

The purpose of this study is to develop a teacher-training program to improve the Technology Home Economics teacher's educational activity effects. To accomplish this goal, this study developed a training program for the Technology Home Economics teacher through the literature study and the research. The conclusion drawn from this study is as follows: First, it needs to develop a systematic teacher training program to raise the professionalism related to the Technology Home Economics teacher's instruction. Second, the developed program for the Technology Home Economics teacher organizes the 46 subjects and 121 educational contents, and this educational program is worthwhile to be facilitated when a teacher-training institution like the Ministry of Eduction, Science and Technology, local education offices and universities organizes a teacher-training program. Third, as the result analyzing teachers' training demands about the educational contents of a Technology Home Economics teacher-training program, it was appeared that the most demand was 'development of professional activities for the Technology Home Economics teacher', and following demands were the 'production of various evaluation tools', the 'effectively epilogue strategy', and the 'technology to increase the learning motivation' in order. Therefore a training program for the Technology Home Economics teacher should deal these educational contents importantly, and a future teacher-training program developer need to refer to them.

• Applied Chemistry for Engineering
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• v.9 no.2
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• pp.286-293
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• 1998

Two methods were used to enhance the adsorption capacity of activated carbons. One is to impregnate activated carbons with chemical compounds which have a good affinity for $CO_2$. The other is to activate by heat-treating after impregnation with KOH on activated carbons(AC). The chemical compounds impregnated on AC were alkali metal, alkaline earth metal, and transition metal chlorides. The adsorption capacity of $CO_2$ on AC impregnated with these metals was less than that of pure AC. These compounds have not the chemical affinity for $CO_2$ and obstruct the micropore of AC. The experiment of breakthrough for $CO_2$ on AC impregnated with KOH showed the increase of the adsorbed amount of $CO_2$ in influent gases containing water vapor. This means that KOH adsorbes $CO_2$ gas. However, the adsorbents impregnated with KOH had not the reproducibility because of the production of $K_2CO_3$ by the reaction of KOH with $CO_2$. The amount of $CO_2$ adsorbed on the heat-treated AC at $800^{\circ}C$ increased with the amount of impregnation. The adsorption capacity of $CO_2$ was the largest when the ratio of weight of KOH to AC equal to 4. The isosteric heat of adsorption was calculated by the equation of Clausius-Clapeyron form adsorption capacity data of $CO_2$ for the temperature change. In addition, the characteristics of $CO_2$ breakthrough curve were surveyed for the change of flow rate and concentration.