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Effects of a Chelated Copper as Growth Promoter on Performance and Carcass Traits in Pigs

  • Zhao, J.;Allee, G.;Gerlemann, G.;Ma, L.;Gracia, M.I.;Parker, D.;Vazquez-Anon, M.;Harrel, R.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.7
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    • pp.965-973
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    • 2014
  • Three studies were conducted to investigate whether a chelated Cu can replace $CuSO_4$ as a growth promoter in pigs. In Exp. 1, a total of 240 piglets (Large White${\times}$Landrace, $7.36{\pm}0.10kg$) were randomly allocated to 1 of 3 treatments with 8 replicates and 10 piglets per pen. Treatments included a NRC control ($CuSO_4$, 6 mg/kg), two Cu supplementations from either $CuSO_4$ or $Cu(HMTBa)_2$ at 170 mg/kg. Pigs fed $Cu(HMTBa)_2$ were 6.0% heavier than pigs fed either the NRC control or 170 mg/kg $CuSO_4$ (p = 0.03) at the end of the experiment. During the 42 days of experimental period, pigs fed $Cu(HMTBa)_2$ gained 9.0% more (p = 0.01), tended to eat more feed (p = 0.09), and had better feed efficiency (p = 0.06) than those fed $CuSO_4$. Compared with the 6 mg/kg $CuSO_4$ NRC control, liver Cu was increased 2.7 times with 170 mg/kg $CuSO_4$ supplementation, and was further increased with $Cu(HMTBa)_2$ (4.5 times, p<0.05). In Exp. 2, a total of 616 crossbred piglets (PIC, $5.01{\pm}0.25kg$) were randomly allocated to 1 of 4 treatments with 7 replicates and 22 piglets per pen. Treatments included a NRC control (from $CuSO_4$), and three pharmaceutical levels of Cu (150 mg/kg) supplemented either from C$CuSO_4$, tri-basic copper chloride ($Cu_2[OH]_3C1$), or $Cu(HMTBa)_2$. Pigs fed $CuSO_4$ or $Cu(HMTBa)_2$ had better feed efficiency (p = 0.01) and tended to gain more (p = 0.08) compared with those fed the NRC control. Pigs fed $Cu_2[OH]_2C1$ were intermediate. Pigs fed $Cu(HMTBa)_2$ had the highest liver Cu, which was significantly higher than those fed ($Cu_2[OH]_3C1$) or the negative control (p = 0.01). In Exp. 3, a total of 1,048 pigs (PIC, $32.36{\pm}0.29kg$) were allotted to 6 treatments with 8 replicates per treatment and 20 to 22 pigs per pen. The treatments included a NRC control with 4 mg/kg Cu from $CuSO_4$, a positive control with 160 mg/kg Cu from $CuSO_4$, and incremental levels of $Cu(HMTBa)_2$ at 20, 40, 80, and 160 mg/kg. During the overall experimental period of 100 days, no benefit from 160 mg/kg $CuSO_4$ was observed. Pigs fed $Cu(HMTBa)_2$ had increased ADG (linear and quadratic, $p{\leq}0.05$) and feed efficiency (linear and quadratic, $p{\leq}0.05$) up to 80 mg/kg and no further improvement was observed at 160 mg/kg for the whole experimental period. Pigs fed 80 mg/kg $Cu(HMTBa)_2$ weighed 1.8 kg more (p = 0.07) and were 2.3 kg heavier in carcass (p<0.01) compared with pigs fed 160 mg/kg $CuSO_4$. In addition, loin depth was increased with increased $Cu(HMTBa)_2$ supplementation with pigs fed 80 mg/kg $Cu(HMTBa)_2$ had the greatest loin depth (p<0.05). In summary, $Cu(HMTBa)_2$ can be used to replace high $CuSO_4$ as a growth promoter in nursery and grower-finisher pigs.

Effects of Amino Acids Fermentation By-product on Fermentation Quality and In situ Rumen Degradability of Italian Ryegrass (Lolium multiflorum) Silage

  • Yimiti, W.;Yahaya, M.S.;Hiraoka, H.;Yamamoto, Y.;Inui, K.;Takeda, M.;Tsukahara, A.;Goto, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.5
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    • pp.633-637
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    • 2004
  • The experiment of silage for preservation of fresh Italian ryegrass (Lolium multiflorum) was carried out to examine whether the fermentation quality and microbial degradation in the rumen can be altered by the treatment of amino acids fermentation byproduct (AFB). The plant was ensiled for 40 days with 4 treatments of different ratios of AFB and sugarcane molasses (SCM) mixture. The treatment 2 (T2, AFB:SCM=100:0) and treatment 3 (T3, AFB:SCM=40:60) silages showed higher (p<0.05) concentrations of lactic acids, lower (p<0.05) pH and dry matter (DM) losses than the Control (T1, none additive) and treatment (T4, AFB:SCM=0:100) silages. The treatments 2 and 3 contained higher (p<0.05) DM and crude protein contents in silages compared to treatments 1 and 4 silages. The NDF, ADF and cellulose contents were also lower (p<0.05) in T2, T3 and T4 silages than T1 silage and fresh material before ensiled. The in situ rumen DM, NDF, ADF, hemicellulose and cellulose degradability was also higher (p<0.05) in T2, T3 and T4 silages than T1 silage, while the highest improvement was achieved with addition of AFB:SCM at level of 40:60 at ensiling. The result in this study indicates that the addition of AFB and SCM additives improved the silage fermentation and cell wall degradability of Italian ryegrass silage.

Bidirectional Transient Voltage Suppression Diodes for the Protection of High Speed Data Line from Electrostatic Discharge Shocks

  • Bouangeune, Daoheung;Choi, Sang-Sig;Choi, Chel-Jong;Cho, Deok-Ho;Shim, Kyu-Hwan
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.14 no.1
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    • pp.1-7
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    • 2014
  • A bidirectional transient voltage suppression (TVS) diode consisting of specially designed $p^--n^{{+}+}-p^-$ multi-junctions was developed using low temperature (LT) epitaxy and fabrication processes. Its electrostatic discharge (ESD) performance was investigated using I-V, C-V, and various ESD tests including the human body model (HBM), machine model (MM) and IEC 61000-4-2 (IEC) analysis. The symmetrical structure with very sharp and uniform bidirectional multi-junctions yields good symmetrical I-V behavior over a wide range of operating temperature of 300 K-450 K and low capacitance as 6.9 pF at 1 MHz. In addition, a very thin and heavily doped $n^{{+}+}$ layer enabled I-V curves steep rise after breakdown without snapback phenomenon, then resulted in small dynamic resistance as $0.2{\Omega}$, and leakage current completely suppressed down to pA. Manufactured bidirectional TVS diodes were capable of withstanding ${\pm}4.0$ kV of MM and ${\pm}14$ kV of IEC, and exceeding ${\pm}8$ kV of HBM, while maintaining reliable I-V characteristics. Such an excellent ESD performance of low capacitance and dynamic resistance is attributed to the abruptness and very unique profiles designed very precisely in $p^--n^{{+}+}-p^-$ multi-junctions.

Physiological and psychological effects of forest healing focused on plant fragrance therapy for maladjusted soldiers

  • Kim, Jihye;Sin, Changseob;Park, Jong-ok;Lee, Hyunchae;Kim, Jihye;Kim, Dohyeong;Kim, Sanghee
    • Journal of People, Plants, and Environment
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    • v.24 no.4
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    • pp.429-439
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    • 2021
  • Background and objective: This study verified the physiological and psychological effects of plant fragrance therapy to analyze whether the therapy has a forest healing effect on maladjusted soldiers. Methods: This study was conducted from March 14 to May 31, 2020 according to the Green Camp curriculum for 2 weeks per recruit class. Thus, plant fragrance therapy was implemented twice each for three recruit classes. Each session of the program was carried out for 4 hours from 1 p.m. to 5 p.m. The venue was the garden within the camp and the forest nearby. Forest healing activities using plant fragrances were led by 1 forest education specialist and 1 assistant. The effects of plant fragrance therapy for maladjusted soldiers were analzed analyzed by quantifying physiological and psychological changes through measuring brain waves and stress levels before and after the therapy. Results: As a result of analyzing BRQ and SQ to determine the physiological and psychological healing effect of plant fragrance therapy, both the left and right brain showed higher BRQ and SQ after the therapy with statistical significance. This indicates that plant fragrance therapy on maladjusted soldiers helped positively stabilize their negative psychological and behavioral dispositions, and stabilization of brain waves lowered physical and mental stress and improved self-regulation and immunity. On the other hand, there was no significant difference in ATO. This is the result of analyzing the stress-coping mechanism. Troubleshooting (t = -2.702, df = 61, p = .009), emotional remission (t = -2.173, df = 61, p = .034), pursuit of help (t = -3.286), df = 61, p = .002), and wishful thinking (t = -3.714, df = 61, p < .001) showed statistically significant results. Conclusion: Applying plant fragrance therapy to maladjusted soldiers positively stabilized their negative psychological and behavioral dispositions and helped their self-regulation. Thus, plant fragrance therapy has psychological and physical forest healing effect on maladjusted soldiers.

Changes of Inorganic Nutrient Contents in Leaf of 'Niitaka' Pear and Inorganic Nutrient Contents of Leaf Influenced by Meteorological Elements (배 신고 품종의 잎 내 무기성분의 시기별 함량 변화와 잎 내 무기성분 함량에 미치는 기후요인)

  • Kim Ik-Youl;Ryu Jong-Ho;Kim Mi-Young
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.3
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    • pp.192-200
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    • 2005
  • This study was conducted to investigate inorganic nutrient contents in spur leaf and shoot leaf of 'Niitaka' pear during the growing season and leaf inorganic nutrient contents as influenced by meteorological elements. The contents of N, P, K, and Mg were decreased in spur leaf during their vegetative growth, while Ca and Mn increased. The contents of N, P, K, Mg, and Fe showed no differences between spur leaf and shoot leaf. However Ca, Mn, Zn, and Cu contents were higher in spur leaf than those in shoot leaf, but B content was lower in spur leaf than those in shoot leaf. The content of N in shoot leaf was positively correlated with mean temperature, whereas negatively correlated with maximum temperature. The content of P in spur leaf was negatively correlated with maximum temperature. The content of Ca in spur leaf was negatively correlated with mean temperature, whereas positively correlated with maximum and minimum temperature. The contents of Mg and B in shoot and spur leaf were positively correlated with mean temperature, whereas negatively correlated with maximum and minimum temperature. The contents of Ca and Mn showed significant differences between spur leaf and shoot leaf at mid-July to early August. These results suggest that sampling is important to distinguish between spur leaf and shoot leaf for diagnosis of nutrient conditions in pear trees.

Molecular Cloning and Characterization of Chitosanase Gene from Bacillus amyloliquefaciene MJ-1 (Bacillus amyloliquefaciens MJ-1 유래의 chitosanase 유전자의 클로닝 및 특성)

  • Park Chan-Soo;Oh Hae-Geun;Hong Soon-Kwang;Park Byung-Chul;Hyun Young;Kang Dae-Kyung
    • Korean Journal of Microbiology
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    • v.42 no.2
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    • pp.142-148
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    • 2006
  • In order to develop chitosanase for the production of chitosan oligosaccharides, a chitosanase-producing bacterium was isolated from the traditional fermented soybean, Meju, and identified as Bacillus amyloliquefaciene MJ-1. The cloned chitosanase gene, 825 bp in size, encoded a single peptide of 274 amino acids with a estimated molecular mass of 30.9 kDa. The deduced amino acid sequence showed significant homology with microbial chitosanases. The recombinant chitosanase was expressed in Escherichia coli upon induction with isopropyl-D-thiogalactopyranoside, and purified using $Ni^{2+}-NTA$ agarose column chromatography. The maximal activity of the recombinant chitosanase is at pH 5.0 and $60^{\circ}C$. The recombinant chitosanase is stable between pH 5.0 and pH 7.0 at $37^{\circ}C$ for 30 min, and more than 75% of the activity still remain at $80^{\circ}C$ for 30 min incubation.

Hypochlorite treatment of polyamide membrane for improved reverse osmosis performance

  • Shao, P.;Kurth, C.J.
    • Membrane and Water Treatment
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    • v.4 no.1
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    • pp.69-81
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    • 2013
  • The pH-dependent inter-conversion of the three free chlorine species ($Cl_2$, HOCl, OCl-) present in the aqueous hypochlorite solution was theoretically investigated. Each species was found overwhelmingly present in a characteristic pH range. Hypochlorite treatment of the polyamide membrane was carried out over these pH ranges and various membrane responses were observed. As pH is less than 8, membrane tends to be N-chlorinated by $Cl_2$ and HOCl, and N-chlorinated membrane showed reduced water permeance and salt rejection. As pH rises to 10-12, $OCl^-$ appears to be the dominating chlorine species. Membrane hydrolysis was found to well interpret the improved water permeance and salt rejection. When the pH is between 8-10, both N-chlorination and hydrolysis contribute to the response of the membrane, and the treated membrane showed improved salt rejection but reduced water permeation. Excessive hydrolysis occurred while the membrane was treated at pH 13 for the much stronger alkalinity.

Electrical and Optical Properties of p-type ZnO:P Fabricated by Ampoule-tube Vapor-state Diffusion

  • So, Soon-Jin;Oh, Sang-Hyun;Park, Choon-Bae
    • Transactions on Electrical and Electronic Materials
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    • v.9 no.1
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    • pp.24-27
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    • 2008
  • ZnO has intensively attracted interest for the next generation of short wavelength LEDs and semiconductor lasers. However, for the development and application of the devices based on this material, the fabrication of p-type ZnO thin films is pivotal. Generally, the process of preparation of ZnO is unavoidably accompanied by the natural donor ions such as interstitial Zn ions and oxygen vacancy ions that show n-type electrical property and make fabrication of p-type ZnO to be a hard problem. On this study, to realize stable high-quality p-type ZnO thin films, the undoped ZnO thin films were diffused with P in vapor state. The ZnO:P thin films showed high-quality p-type properties electrically and optically.

Optimized Germination Conditions and Human p53 Expression of Rice Embryo (쌀눈 발아의 최적조건 확립 및 p53 항암 유전자의 발현)

  • Pih, Kyung-Tae;Choi, Ju-Youn;Kim, Keun-Cheol
    • Journal of Life Science
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    • v.25 no.2
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    • pp.158-163
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    • 2015
  • Rice embryo is more abundant than endosperms in nutrients such as proteins, lipids, and vitamin B1. In this study, we constructed p53 plasmid that could be expressed in a plant system, and investigated optimal germination conditions in a variety of media. For construction of p53 plasmid, we performed p53 amplification from pCDNA-p53, subcloned to TA cloning vector, and then reconstructed into pGEM-CaMV plant expression vector. On the other hand, we prepared a variety of imbibition buffers and complete media for efficient germination of the rice embryo. Imbibition buffers prepared with different concentrations of salt or detergent showed no significant effect on germination efficiency. We prepared further culture media, such as solid agar, liquid media, and paper towel to establish the optimal conditions. Rice embryo showed germination rates of more than 70% in the solid medium, more than 60% in the paper towel medium, but less than 25% in liquid media, although germination rate did not differ with varying concentrations of salt and sucrose in culture media. Under the optimal germination conditions, we introduced the p53 plasmid using imbibition method, and finally detected human p53 gene expression in the germinated rice embryo. This method might present a novel, practical approach for evaluating efficient gene expression utilizing imbibition method in rice embryo.

A study on exposed aggrigate P.C. in field plant cast - case study for core building, VOTRAKON project - (골재노출 P.C제품의 현지생산에 관한 소고 -보트라콘 프로젝트의 코아빌딩을 중심으로-)

  • 이학영
    • Journal of the Korean Professional Engineers Association
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    • v.19 no.2
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    • pp.34-42
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    • 1986
  • These brief studies and reports of the use of exposed aggrigate precast concrete in practice are combined with a description of some notable co-ordination such as shop drawing, manufacturing and erection in the field construction. Above mentioned exposed aggrigate P. C. means architectural P. C. which was used to the VOTRAKON site in Riyadh, Saudi Arabia. Fabricated P. C. on the site without autoclaving and steam curing plant had been successfully carried out on this project-core building, conference room and others. The project designed by HOPE/VTN international INC. which is located in San Diego, California U.S.A. stands for vocational training and related support facilities contract. We had to submit shop drawings showing complete information for fabrication and installation of P. C. unit reinforcement. Also we should be indicated member dimension and cross section, location size and type of necessary for erection. We can delineate the following characteristic results. One of the most important things how to handle exposed aggrigate P.C. unit as specified was quality assurance and co-ordination for shop drawing, manufacturing and erection.

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