• Korean Journal of Poultry Science
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• v.44 no.3
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• pp.199-209
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• 2017

Mitogen-activated protein kinase (MAPK) signaling pathways play a key role in innate immunity, inflammation, cell proliferation, cell differentiation, and cell death. The main objective of this study was to investigate the expression level of candidate MAPK pathway genes in the intestinal mucosal layer of two genetically disparate chicken lines (Marek's disease-resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE). Using high-throughput RNA sequencing, we investigated 178 MAPK signaling pathway related genes that were significantly and differentially expressed between the intestinal mucosal layers of the NE-afflicted and control chickens. In total, 15 MAPK pathway genes were further measured by quantitative real-time PCR(qRT-PCR) and the results were consistent with the RNA-sequencing data. All 178 identified genes were annotated through Gene Ontology and mapped onto the KEGG chicken MAPK signaling pathway. Several key genes of the MAPK pathway, ERK1/2, JNK1-3, p38 MAPK, MAP2K1-4, $NF-{\kappa}B1/2$, c-Fos, AP-1, Jun-D, and Jun, were differentially expressed in the two chicken lines. Therefore, we believe that RNA sequencing and qRT-PCR analysis provide resourceful information for future studies on MAPK signaling of genetically disparate chicken lines in response to pathogens.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.215-222
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• 2015

The radish (Raphanus sativus L.) is an important Brassicaceae root vegetable crop worldwide. Several studies have been conducted concerning radish breeding. There are major challenges to prevent premature bolting in spring plantings. Here, we performed the characterization of two inbred radish lines which vary in bolting time. "Late bolting radish" (NH-JS1) and "early bolting radish" (NH-JS2) were generated by a conventional breeding approach. The two inbred lines showed different bolting phenotypes depending on vernalization time at $4^{\circ}C$. NH-JS1, the late bolting radish, was less sensitive to cold treatment and the less sensitivity was inversely proportional to the duration of the vernalization. We also measured gene expression levels of the major bolting time related genes in the NH-JS1 and NH-JS2 lines. RsFLC1 plays a central role in the timing of flowering initiation. It is a strong repressor and it's transcript is highly expressed in NH-JS1 compared to NH-JS2 under no treatment and vernalization conditions. RsFRI, a positive regulator of RsFLC, is also highly expressed in NH-JS1 compared to NH-JS2 regardless of vernalization. In contrast, RsSOC1, suppressed by FLC as a floral integrator gene, showed the most difference, a 5-fold increase, between NH-JS1 and NH-JS2 under vernalization conditions. From these results, we conclude that NH-JS1 showed a late flowering phenotype after cold treatment due to the expression differences of flowering time regulator genes rather than difference sensitivity to cold. These results may be useful to understand the control mechanisms of flowering time and may help identify molecular markers for selecting late bolting trait in radish.

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.33-38
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• 2001

Protoplasts isolated from inbred lines of Brassica oleracea L. var capitata (cabbage) and Brassica campestris L. ssp. pekinensis (Chinese cabbage) were fused by PEG-mediated method, and somatic hybrid cells were differentiated into plants. for the identification of somatic hybrid plants, ploidy level, plant morphology, and cytological analysis were performed. All of the regenerated plants derived from fused protoplasts were shown to be 2X-4X, or higher ploidy level, presumably due to somatic hybridization or chromosome doubling. The morphology of leaves, petioles, and flowers showed an intermediate phenotype between Chinese cabbage and cabbage. Chromosome numbers in these somatic hybrids ranged mostly from 33 to 38. According to Genomic in situ hybridization (GISH) pattern, signals from both fusion parents of B.campestris or B.oleracea were detected in different colors when chromosomes of putative somatic hybrids were observed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.254-255
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• 2005

• The Korean Journal of Applied Statistics
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• v.14 no.1
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• pp.59-70
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• 2001

이면교배 실험은 식물이나 동물의 교배를 통해 자식계통의 유전적인 성질을 조사하는데 중용한 역할을 한다. 이면교배에 관한 기존의 연구에서는 p개의 자식계통은 하나의 집합에 속하는 것으로 간주하였다. 이와 달리 새로 육성된 자식계통의 유전적 성질을 평가하고자 하는 경우에는, p개의 자식계통이 각각 p$_1$과 p$_2$개의 자식계통을 갖는 두 개의 집합으로 분리되는 형태의 교배실험을 사용한다(단, p=p$_1$+p$_2$). 이에 본 연구에서는 자식계통이 두 집단으로 분리된 경우에 대해서 Kempthorne의 합동식을 이용하여 두 군간 교배에 대한 블록디자인을 설계하고, 이에 대한 평균효율인자를 계산하는 방법을 제안하고자 한다.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.133-133
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• 2003

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.244-245
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 1996.10a
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• pp.24-25
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• 1996

• Proceedings of the Korean Society of Crop Science Conference
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• 1996.10a
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• pp.28-29
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• 1996

• Proceedings of the Korean Society of Crop Science Conference
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• 1996.10a
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• pp.30-31
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• 1996