• Toxicological Research
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• v.34 no.1
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• pp.55-63
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• 2018

As a part of general toxicity studies of Enterococcus Faecalis 2001 (EF 2001) prepared using heat-treatment bacillus mort body EF 2001 in mice, this study examined the toxicity of EF 2001 in single and repeated administrations following the previous report in order to apply this product to preventive medicine. The safety of oral ingestion of EF 2001 was examined in 6-week-old male and female ICR mice with 1,000 mg/kg, 3,000 mg/kg and 5,000 mg/kg body weight/day administrated by gavage of the maximum acceptable dose of EF 2001. The study was conducted using distilled water as a control following the methods for general toxicity studies described in the "Guidelines for Non-clinical Studies of Pharmaceutical Products 2002". As a control, 1) observation of general conditions, 2) measurement of body weight, 3) determination of food consumption, 4) determination of water consumption, 5) blood test and urinalysis and 6) pathological examination were performed for the administration of EF 2001. Mice received EF 2001 for 13 weeks and results were compared with those of the control group that received distilled water. The results of the above examinations revealed no significant differences between control and EF 2001 groups for both males and females. Thus, no notable toxicity was confirmed with single and repeated oral administrations of EF 2001. Oral administration in the above doses did not result in abnormal symptoms or death during the observation period. No abnormalities in blood cell count or organ weights were seen. Without any evidence of toxicity to cells and organs, EF 2001 is speculated to not adversely affect living organisms. The 50% lethal dose of EF 2001 with oral administration in mice is estimated to be greater than 5,000 mg/kg body weight/day for both male and female mice. Therefore, $LD_{50}$ value for animals was 5,000 mg/kg or more.

• Biomolecules & Therapeutics
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• v.20 no.1
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• pp.81-88
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• 2012

Histone deacetylases (HDACs) are enzymes involved in the remodelling of chromatin, and have a key role in the epigenetic regulation of gene expression. Histone deacetylase (HDAC) inhibitors are emerging as an exciting new class of potential anti-cancer agents. In recent years, a number of structurally diverse HDAC inhibitors have been identifi ed and these HDAC inhibitors induce growth arrest, differentiation and/or apoptosis of cancer cells in vitro and in vivo. However, the underlying molecular mechanisms remain unclear. This study aimed at investigating the anti-tumor activity of various HDAC inhibitors, IN-2001, using T47D human breast cancer cells. Moreover, the possible mechanism by which HDAC inhibitors exhibit anti-tumor activity was also explored. In estrogen receptor positive T47D cells, IN-2001, HDAC inhibitor showed anti-proliferative effects in dose-and time-dependent manner. In T47D human breast cancer cells showed anti-tumor activity of IN-2001 and the growth inhibitory effects of IN-2001 were related to the cell cycle arrest and induction of apoptosis. Flow cytometry studies revealed that IN-2001 showed accumulation of cells at $G_2$/M phase. At the same time, IN-2001 treatment time-dependently increased sub-$G_1$ population, representing apoptotic cells. IN-2001-mediated cell cycle arrest was associated with induction of cdk inhibitor expression. In T47D cells, IN-2001 as well as other HDAC inhibitors treatment significantly increased $p21^{WAF1}$ and $p27^{KIP1}$ expression. In addition, thymidylate synthase, an essential enzyme for DNA replication and repair, was down-regulated by IN-2001 and other HDAC inhibitors in the T47D human breast cancer cells. In summary, IN-2001 with a higher potency than other HDAC inhibitors induced growth inhibition, cell cycle arrest, and eventual apoptosis in human breast cancer possibly through modulation of cell cycle and apoptosis regulatory proteins, such as cdk inhibitors, cyclins, and thymidylate synthase.

• Journal of the Korean Dietetic Association
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• v.9 no.1
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• pp.47-56
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• 2003

Currently, dietary life in Korea is rapidly changing. New food from other countries introduced to the diet with changing economic and social environment. The number of meals away from home is increasing. Also, number of students who served by the school food service are increasing. In this paper, the menu in the school food service are compared between 1995 and 2001. Analysis was done to see changes in the use of Korean traditional foods. Between July, 2001 and August, 2001, by mail 103 schools were surveyed to analyzed menu. Menu from 2001 survey are compared with menu from 1995 survey. The results are following; 1) New menus are introduced in 2001 compared with 1995. 2) The number of menu using animal foods are increasing in 2001. 3) Among cooking methods, stir frying, and deep frying, are used more in 2001 than that of 1995. 4) The number of Korean dish served are decreased in 2001. 5) Students favorite dish are changed. Students like dishes cooked with deep frying method and western foods. With these results, menu of school food services become more westernized and using more frying methods because of students' taste preference. These trend are not recommendable for the students health, because it is known that Korean traditional dishes are more healthful. There should be more research and effort to keep Korean tranditional foods in the menu of the school food service for students health.

• Korean Journal of Environmental Biology
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• v.23 no.1
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• pp.57-63
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• 2005

The phytoplankton community, environmental factors, and rainfall were investigated from July to October in 2001 and 2003 on Dam site in Daechung Reservoir. The monthly average rainfall in the investigated period were 91.3 and 265.3 mm in 2001 and 2003, respectively. The maximum chlorophyll a concentration was observed higher at 131.5 ㎍ L/sup -1/ in 2003 than at 45.4 ㎍ L/sup -1/ in 2001. The cyanobacterial number in 2001 was counted up to over 200,000 cells mL/sup -1/, which was much higher than the maximum number of 49,000 cells mL/sup -1/ in 2003. The relative abundance of cyanobacteria in the phytoplankton community was about 97% in 2001 and 74% in 2003. Microcystis spp. were absolutely dominant species in 2001, while a couple of cyanobacteria such as Oscillatoria spp., Phormidium spp. Chroococcus spp. and Microcystis spp. were dominant species in 2003. Consequently, it seemed that rainfall affected the diversity of phytoplankton species and decreased the density of bloom-forming cyanobacteria.

• Health Policy and Management
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• v.27 no.4
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• pp.372-374
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• 2017

Korea is one of the fastest aging country in the world. The increase in health expenditure is an inevitable problem in an aging country. We examined trend of benefit expenditure in National Health Insurance (NHI) by age group during 2001 to 2016. The benefit expenditure of NHI was 13.0 trillion won (2001) and 48.7 trillion won (2016); that is 3.76 times to 2001. This rapid increase was mostly due to people aged 65 and over. Proportion of beneficiaries aged 65 and over was 6.9% (2001) and 13.7% (2016), benefit expenditure per capita aged 65 and over was 279 thousand won (2001) and 960 thousand won (2016), and utilization days per capita aged 65 and over 21.44 (2001) and 30.23 (2016). This phenomenon was more pronounced in 75 or 85 aged and over. To contain the health costs for older people, the NHI system should be reformed.

• The KIPS Transactions:PartA
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• v.10A no.3
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• pp.239-246
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• 2003

The Verilog parser libary for IEEE Verilog 1364-2001 Standard is developed in the paper. The lexer and scanner are developed and tested to handle "Yerilog-2001" which is the first major update to the Verilog language since its inception in 1984. Also the newly developed XML intermediate format for Verilog-2001 is presented. By using the XML intermediate, it allows the portable and scalable development of various kinds of verilog applications that are mainly focused on semantic manipulation.ipulation.

• Research in Plant Disease
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• v.8 no.4
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• pp.239-244
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• 2002

Among 34 isolates of Colletotrichum spp., 20 from red pepper and 14 from apple, only one isolate 2001-45 was identified as the isolate resistant to sterol biosynthesis inhibiting fungicides. EC$_{50}$ values of the isolate 2001-45 were 153.5, 42.7, 34.0, and 17.1 $\mu\textrm{g}$/ml for myclobutanil, tebuconazole, hexaconazole and nuarimol, respectively, The resistance factor of the isolate 2001-45 against the other isolate 2001-44 to 4 above fungicides was ranged from 17 to 57. However, EC$_{50}$ value of the 2001-45 for prochloraz was 0.07 $\mu\textrm{g}$/ml, which was lower than those of the 2001-44 and the isolate JC24. For the fitness test of the 2001-45, mycelial growth, sporulation on PDA and pathogenicity on fruits were investigated. No difference in mycelial growth was found between 2001-45 and 2001-44, but great difference in sporulation. No symptom was developed by 2001-45 even by wound inoculation of pepper fruit. Therefore, this study indicated that the isolate 2001-45 was inferior to the other isolates in the fitness, although the isolate 2001-45 was highly resistant to sterol biosynthesis inhibiting fungicides.

• Journal of Nutrition and Health
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• v.38 no.4
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• pp.320-326
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• 2005

This study was conducted to estimate dietary choline intake of Koreans according to gender, age and region by using the data on per capita food intake in 1998 and 2001 and the data on choline contents of foods customarily eaten by Koreans. Sixty-four and 97 kinds of food items were used for the estimation of choline intake in 1998 and 2001, respectively. And these corresponded to approximately $90\%$ of the total amount of food intake and energy intake. Based on these food items and choline content, per capita choline intake of Koreans was estimated to be 623.0 mg and 602.4 mg in 1998 and 2001 respectively , 687.2 mg and 659.1 mg in 1998 and 2001 for male, 560.2 mg and 551.0 mg in 1998 and 2001 for female. While the number of foods considered in estimation was higher in 2001 than 1998, choline intake was lower in 2001. The estimated per capita choline intake of male population was higher than that of female population by 108-127 mg, possibly due to the difference in total amount of food intake. The age group with the highest choline intake was different between 2 time points. Although adolescents of 13- 19 years showed the highest intake (712.0 mg) in 1998, mid-aged adults of 30-49 years showed the highest choline intake (662.2 mg) followed by adolescents (645.7 mg) in 2001. In regional comparison, choline intake of the rural population (588.7 mg and 588.3 mg in 1998 and 2001 respectively) was lower than that of population in small to medium-size cities (625.1 mg and 603.9 mg) or in metropolitan cities (637.7 mg and 602.8 mg). This result revealed that per capita choline intake of the Korean population falls within the adequate range with some differences by age, sex and residing area.

• Journal of Korean Society for Quality Management
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• v.29 no.4
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• pp.65-81
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• 2001

ISO 9001:1994 / KS A 9001:1998 was recently revised as ISO 9001:2000 / KS A 9001:2001, so as to be used in non-manufacturing companies as well. In this paper, a model for quality management system in public administration sector was proposed by customizing ISO 9001:2000 / KS A 9001:2001. The proposed model is compatible with ISO 9001:2000 / KS A 9001:2001, and expected to be used as a guideline for those people who are involved in establishing quality administration system in public sector.

• Korean Institute of Interior Design Journal
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• v.22 no.6
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• pp.130-139
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• 2013

The purpose of this study is to analyze the characteristics of Korean sofa design in 2013 and understand the change of it in comparison with the characteristics of sofa in 2001. For the sake of it, this study analyzed the styles, forms, materials, colors and composition of 220 sofas posted on 7 main furniture companies' internet homepage of Korea, and used the results of the previous study for the materials in 2001. As the results of analysis, the style of furniture was almost modern and there was much of lineal in the form of sofa, rather than curved. The percentage of using leather for main material of sofa was greatly increased compared with 2001. In case of minor material, there was difference in each company in percentage of using wood and metal. The use of brown color was greatly increased compared with 2001, and the diversification of color use was reduced. The percentage of using a single color gets increased compared with 2001, but the cases of strong contrast were more than that of weak contrast. The percentage of sofa for 3 people gets lowered compared with 2001, and the case of sofa for 4 people gets greatly increased. In case of auxiliary component, the use of couch and stool, which were almost not used in 2001, gets more increased than sofa for single, with many cases of using the corner, though small. In conclusion, the characteristics of Korean sofa design are diversification of the sofa configuration, the tendency of high-quality sofa, and simplification and concentration of the sofa design.