• Korean journal of applied entomology
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• v.18 no.4 s.41
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• pp.153-160
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• 1979

The present experiments were carried out to reduce the population density of adult pine gall midge, Thecodiplosis japonensi,s UCHIDA et INOUYE, by means of spray the sticky agent C-4, on the ground, herbacious plantation and foliar leaf of pine trees at Sanbonli,. Anyang, Kyungido. And also the 3 periods of treatment, such as before 2 weeks( I ), before 1 week (II) from the peak emergence period and peak emergence period (III) were applied. The experimental results obtained were as follows. (1) The formula of selected sticky agent C-4 was $70\%$ of castor oil, $25\%$ of damar resin and $5\%$ of carnauba wax, and it showed the best both on stickiness and duration, and lower phytotoxicity to the host plant. (2) The reduction of population densities of the adult PGM were $68\%$ and 78f: in the G-I and G-II treatment plot respectively, which compared to control, on the ground spray. (3) And the reduction of population densities were $63\%$ and about $90\%$ in the P-I and P-II plot respectively when the agent was sprayed on the herbacious plantation. (4) The rates of gall formation were $32.8\%,\;40.8\%\;and\;59.4\%$ in the spray plots of F-I, F-II, and F-III respectively, and there was no significant difference among the upper, middle and lower parts of the treated host plant in the rates of gall formation. (5) The effective stage of sticky agent application were considered as before one week from the peak emergence period in tile all types of treatment.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1993.06a
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• pp.842-844
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• 1993

We present a F.L.C. (Fuzzy Logic Controller) to control of the oscillation frequency of a V.C.X.O. (Voltage Controled Crystal Oscillator). This F.C.X.O. maintains stable its oscillation frequency inside a range of 1 ppm (one part per millon), with temperature between -55$^{\circ}C$ to＋75$^{\circ}C$.

• Herbal Formula Science
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• v.26 no.1
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• pp.13-26
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• 2018

Objectives : This study was carried out to enhance the activity of Iksuyoungjingo, which has the tonify Qi, nourish Yin effect of oriental medicine, and to study the effect of the prescriptions on the immunological activities. Methods : Immunosuppression was induced by methotrexate (MTX) 2 mg / kg, the experimental group was divided into IYGgami-I, IYGgami-I-F, IYGgami-II and IYGgami-II-F groups. Each prescription was administered with drinking water for 20 days, and body weight was measured every 5 days during this period. Leukocyte, $TNF-{\alpha}$, IL-2, IL-6, IgE, spleen weight and body weight were measured Results : In the changes of $TNF-{\alpha}$, IL-2 and IL-6 as pro-inflammatory elements, all of the experimental groups showed a significant increase compared to the control group. In the IgE changes, the IYGgami-I-F, IYGgami-II and IYGgami-II-F groups showed a significant decrease compared to the control group. In the changes of spleen weight, the IYGgami-II-F group showed a significant increase compared to the control group. In the changes of WBC and lymphocytes, the IYGgami-I-F group showed a significant increase compared to the control group. Conclusions : From the above results, it can be observed that the efficacy against immunity is exerted in all of the preparations, and it was confirmed that the efficacy was maintained constant even when utilizing the Rehmannia glutinosa Residue and Poria cocos Bark, and that a more beneficial effect can be exerted in the effectiveness when the fermentation is carried out.

• The Plant Pathology Journal
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• v.38 no.3
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• pp.254-260
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• 2022

Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.

• Research in Plant Disease
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• v.18 no.2
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• pp.73-79
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• 2012

Phytophthora katsurae is a fungal pathogen responsible for chestnut ink disease. We designed two duplex primer sets (SOPC 1F/1R+KatI 3F/5R, SOPC 1-1F/1-1R+KatI 3F/5R) to detect P. katsurae. SOPC 1F/1R and SOPC 1-1F/1-1R primer pairs were designed for sequence characteristic amplification regions (SCAR) marker, and KatI 3F/5R primer pair was used for P. katsurae-specific primer designed from internal transcribed spacer (ITS) region. To assess the sensitivity of duplex PCR, genomic DNA was serially diluted 10-fold to make the final concentrations from 1 mg/ml to 1 ng/ml. The sensitivity for two primer sets were 1 ${\mu}g/ml$ and 100 ng/ml, respectively. To find detection limits for zoospores of P. katsurae, each zoospore suspension was serially diluted 10-fold to make the final concentrations from $1{\times}10^6$ to $1{\times}10^2$ cells/ml, and then DNA was extracted. The limits of detection for all of two primer sets were $1{\times}10^5$ cells/ml. All of two primer sets were specific to P. katsurae in PCR detection and did not produce any P. katsurae-specific PCR amplicons from other 16 Phytophthora species used as the control. This study shows that duplex PCR using two primer sets might be a useful tool for rapid and efficient detection of P. katsurae.

• Parasites, Hosts and Diseases
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• v.60 no.3
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• pp.201-205
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• 2022

Babesia microti is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, B. microti cytochrome c oxidase subunit I (cox1) was selected as the target gene, multiple primers were designed, and optimized by a recombinase-aided amplification (RAA) assay. The optimal primers and probe were labeled with fluorescein. The sensitivity of fluorescent RAA (fRAA) was evaluated using gradient diluents of the cox1 recombinant plasmid and genomic DNA extracted from whole blood of B. microti infected mice. The specificity of fRAA was assessed by other transfusion transmitted parasites. The analytical sensitivity of the fRAA assay was 10 copies of recombinant plasmid per reaction and 10 fg/µl B. microti genomic DNA. No cross-reaction with any other blood-transmitted parasites was observed. Our results demonstrated that the fRAA assay would be rapid, sensitive, and specific for the detection of B. microti.

• Mycobiology
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• v.50 no.6
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• pp.475-486
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• 2022

The antifungal activity of thymol against Aspergillus awamori F23 and Botrytis aclada F15 in onions was examined through direct treatment with amended media and gaseous treatment with I-plates (plastic plates containing central partitions). The protective and curative control efficacy of thymol was examined 24 h before and after the inoculation of onion bulbs with the fungal isolates. Mycelial growth, sporulation, and spore germination of the isolates were inhibited on potato dextrose agar amended with various concentrations of thymol or acetic acid (positive control). Overall, thymol produced a stronger inhibitory effect on the mycelial growth and development of the isolates than acetic acid. Following gaseous treatment in I-plates, mycelial growth, sporulation, and spore germination of the isolates were inhibited at higher concentrations of thymol or acetic acid; however, acetic acid showed a little effect on the sporulation and spore germination of the isolates. Following the treatment of onion bulbs with 1000 mg L^-1 of thymol 24 h before and after fungal inoculation, lesion diameter was greatly reduced compared with that following treatment with 0.5% ethanol (solvent control). Onion bulbs sprayed with thymol 24 h before fungal inoculation generally showed reduced lesion diameters by isolate F23 but not in isolate F15 compared with those sprayed 24 h after fungal inoculation. Collectively, thymol effectively inhibited the growth and development of A. awamori and B. aclada on amended media and in I-plates. In addition, spraying or fumigation of thymol is more desirable for effectively controlling these postharvest fungal pathogens during long-term storage conditions.

• 제어로봇시스템학회:학술대회논문집
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• 1994.10a
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• pp.307-312
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• 1994

The authors have been devoted to researches on fuzzy theories and their applications, especially control theory and application problems, for recent years. In this paper, the authors present results on a comparison of optimal solutions between ones of an ordinary-typed mathematical linear programming problem(O.M.I.P. problem) and ones of a Zimmerman-typed fuzzy mathematical linear programming problem (F.M.L.P. problem), and comment about the sensitivity (differences and fuzziness on between O.M.L.P. problem and F.M.L.P. problem) on optimal solutions of these mathematical linear programming problems.

• Phonetics and Speech Sciences
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• v.2 no.4
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• pp.229-238
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• 2010

This study investigates the differences in acoustic parameters in vowel space across hearing loss, gender and vowels. The parameters include F1, F2, Euclidean Distance between vowels, and vowel triangular area comprised of /i/, /a/ and /u/. For this study, 20 hearing-impaired and normal hearing adults as a control group were asked to read 7 Korean vowels (/a, $\wedge$, o, u, w, i, $\varepsilon$/). Subjects' readings were recorded by NasalView and analyzed by Praat. Results showed that F1 were significantly higher in the hearing impaired group than in the normal hearing group, higher in the female group than in male group, and higher in low vowels than in high vowels. And the means of F2 was significantly higher in the hearing impaired group than in normal hearing group, higher in high vowels than in low vowels, and there was no difference between male and female group. Secondly, Euclidean distance between vowels was significantly shorter in the hearing-impaired group than in the normal group. Finally, acoustic vowel space area was significantly smaller in the hearing-impaired group than in the normal hearing group. The hearing-impaired group showed that front vowels tended to be backed and back vowels to be fronted.

• The Korean Journal of Elementary Counseling
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• v.4 no.1
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• pp.215-236
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• 2005

The Purpose of this study is that self-growth program searches effect self-concept and peer-relationship with elementary school student, and advance following assumption to achieve this purpose and achieved study. First, self-concept point of experiment group students who execute self-growth program will be improved is meaning than self-concept point of control group students. Second, peer-relationship point of experiment group students who execute self-growth program will be improved is meaning than peer-relationship point of control group students. Third, effect that self-growth program gets to self-concept may be meaning difference according to sex. Fourth, difference that impact that self-growth program gets in peer- relationship is meaning according to sex may join. Chose fifth-year student 2 class 68 people (experiment group n=34, control group n=34) in I primary school locating to Jeonrabuk-do Iksan-si to verify above construction for study target. Disposal about experiment group executed over 10th for 60-80 minute 2 times in a week because investigator uses reconstructing self-growth program with virtue research paper such as learning program for own growth of Lee-Hyeong-Deuk (1998). In order to verify the effect after experiment, 1 collected materials for estimation by providing the subject children with questionaires about self-concept and peer-relationship before and after the experiment, and then analyzed the average differences in number of marks between the experiment group and the control group before and after the experiment through and by using One-Way ANOVA, and SPSS 11.0 program. The following is the result what I obtained from the above study. First, there was significant difference is between average difference before and after of experiment group and control group which execute self-growth program in self-concept elevation ($F_{(1,66)} =28.734$, p <.001). From the sub-variable, there was significant difference in academic self ($F_{(1,66)}=6.423$, p<.05), Social Self ($F_{(1,66)}=48.331$, p<.001), Physical Self ($F_{(1,66)}=11.074$, p <.01), sentimental self ($F_{(1,66)}=9.402$, p <.01) Second, there was significant difference is average difference before and after of experiment group and control group which execute self-growth program in peer-relationship promotion ($F_{(1,66)}=24.109$, p <.001). From the sub-variable there W3S Significant difference in trust ($F_{(1,66)}=14.507$, p<.001), respect ($F_{(1,66)}=15.271$, p <.001). Third, there was expose that significant difference does not exist in average self-concept before and after by sex of experiment group which executes self-growth program, and was not shown significant difference in sub-vairable. Fourth, there was expose that significant difference of whole peer- relationship and in respect of sub-variable in average peer-relationship before-after by sex of experiment group which execute self-growth program, but significant difference did not appear in trust. Could get conclusion that self-growth program is effect in elementary school student self-concept elevation and peer-relationship promotion according to these study finding, and confirmed possibility that self-growth program may contribute to change emotional special quality of children positively in education spot.