• Animal cells and systems
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• v.5 no.2
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• pp.133-137
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• 2001

Long terminal repeats (LTRs) of the human endogenous retrovirus K family (HERV-K) have been found to be coexpressed with sequences of genes closely located nearby. We examined transcribed HERV-K LTR elements in human brain tissue. Using cDNA synthesized from mRNA of the human brain, we performed PCR amplification and identified ten HERV-K LTR elements. These LTR elements showed a high degree of sequence similarity (92.4-99.7%) with the human-specific LTR elements. A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-K LTR elements could be divided into two groups through evolutionary divergence. Some HERV-K LTR elements (HKL-B7, HKL-B8, HKL-B10) belonging to the group II from human brain cDNA were closely related to the human-specific HERV-K LTR elements. Our data suggest that HERV-K LTR element are active in the human brain; they could conceivably play a pathogenic role in human diseases such as psychosis.

• Molecules and Cells
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• v.40 no.9
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.

• Molecules and Cells
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• v.27 no.3
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• pp.313-319
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• 2009

The dual-vector system-II (DVS-II), which allows efficient display of Fab antibodies on phage, has been reported previously, but its practical applicability in a phage-displayed antibody library has not been verified. To resolve this issue, we created two small combinatorial human Fab antibody libraries using the DVS-II, and isolation of target-specific antibodies was attempted. Biopanning of one antibody library, termed DVFAB-1L library, which has a $1.3{\times}10^7$ combinatorial antibody complexity, against fluorescein-BSA resulted in successful isolation of human Fab clones specific for the antigen despite the presence of only a single light chain in the library. By using the unique feature of the DVS-II, an antibody library of a larger size, named DVFAB-131L, which has a $1.5{\times}10^9$ combinatorial antibody complexity, was also generated in a rapid manner by combining $1.3{\times}10^7$ heavy chains and 131 light chains and more diverse anti-fluorescein-BSA Fab antibody clones were successfully obtained. Our results demonstrate that the DVS-II can be applied readily in creating phage-displayed antibody libraries with much less effort, and target-specific antibody clones can be isolated reliably via light chain promiscuity of antibody molecules.

• Journal of the Korean Society for information Management
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• v.36 no.1
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• pp.73-94
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• 2019

The purpose of this study is to investigate the quality of service that affects satisfaction of special library users and to suggest ways to improve the function and role of special library. The data were collected by survey and interview method conducted with 111 library users in 6 local research institute using modified questionnaires based on LibQUAL+. The results revealed that human quality factor had more influence on library service satisfaction than space and information quality factor. Also, there were statistically significant influence between library service satisfaction and three sub service factors, such as service attitude, space convenience, and information usability. The interviewers indicated problems of special library service in lack of data, inactivation of digital data, space problem, data sharing problem, and lack of experts. Finally this study proposed several suggestions to improve service quality of special library of local autonomous entity research institution.

• Journal of the Korean housing association
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• v.14 no.3
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• pp.127-136
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• 2003

The purpose of this study is to establish concept about a plan direction of preschooler's library, and provide a design type of preschooler's library. As a result, the following findings were obtained: 1) For the preschooler's reading area, it should be segregated between schooler and preschooler. Because there is a difference between a library use action and a development characteristic, it needs to be segregated to avoid conflict using library for both of those groups. 2) The space composition of preschooler's library stands up, and of a tube considers a traffic line of a user according to the function and systematizes a child, and to cause an interest of preschoolers. 3) Furnitures and equipment of preschooler's library must arrange in consideration of human body size of a user in a tube.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2005.10a
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• pp.219-220
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• 2005

This present study purposed to propose a plan of building a future oriented school library that supports teachers' teaching with various multimedia and Internet materials applicable in class immediately, helps students' self directed learning activities, and plays its role as a cultural center of the local community. As for research methods, this study examines the basic directions and characteristics of school libraries defined by the Ministry of Education and Human Resources Development, and investigates the current state of school libraries. In addition, we survey cases of library plans in Incheon, and suggest standards for planning and propose a plan. Ultimately, we establish a library plan for S Elementary School in Incheon and execute the plan and, in the course, derive ideas for satisfying students' demands with regard to their sentiment, stable and flexible layout and flow lines, facilities and space within the library, etc. What is more, we create indexes for library planning so that school libraries can contribute to various cultural activities and the development of local community beyond their functions as school library facilities.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.1
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• pp.377-381
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• 2012

The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.829-832
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• 2001

Phage display technique as a new antibody production method can express the protein on the minor coat of phage particle in a library constructed by utilizing a recombination of genes coding the variable regions of immunoglobulin. This new method is particularly advantageous in producing antibodies against toxic substances and compounds with low immunogenicities. We first confirmed the concept of antibody expression on the phage particle by selecting a positive control of the phage library (e.g., Griffin.l donated from MRC center in England). The library was then employed to produce antibodies specific to human serum albumin via repetitive bio-panning procedure. The mean affinity of the antibodies selected gradually increased along with the number of bio-panning, which demonstrated that the phage display method could produce monocloanl antibodies with high affinities.

• Journal of the Korean Society for Library and Information Science
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• v.38 no.3
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• pp.277-296
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• 2004

This paper is to analyze the impact of changes n academic library management environment on information services. For this purpose. based on literature reviews. various factors affecting information services due to changes in management environment such as human resources, information resources, facilities, budget and information technologies are investigated. Also, in order to substantiate this work, librarians in diverse university libraries were surveyed. According to our survey results, The conclusion is as follows. That is, the most to least affecting factors on information services in university libranes are human resources, information technologies and facilities, respectively.

• Journal of Korean Library and Information Science Society
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• v.36 no.1
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• pp.459-477
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• 2005

The purpose of this study is to investigate a fundamental model for categories and relationships among concepts in library and information science field in order to construct an ontology based upon the knowledge construct of human being. Questionnaire and interview were involved for this study, The results shows that students' knowledge structure was different from KDC and DDC schemes. Students had an object oriented knowledge structure and provided four relationship models. These model could provide a fundamental data for ontology construction. Also, it shows that student academic grades influenced their knowledge structures.