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http://dx.doi.org/10.7314/APJCP.2012.13.1.377

Screening Peptides Binding Specifically to Colorectal Cancer Cells from a Phage Random Peptide Library  

Wang, Jun-Jiang (Department of Gastroenterology Surgery, Guangdong General Hospital)
Liu, Ying (Department of Obstetrics and Gynecology, Guangdong General Hospital)
Zheng, Yang (Department of Gastroenterology Surgery, First Affiliated Hospital of Shan-tou University Medical College)
Liao, Kang-Xiong (Department of General Surgery, the Jieyang People's Hospital)
Lin, Feng (Department of Gastroenterology Surgery, Guangdong General Hospital)
Wu, Cheng-Tang (Department of General Surgery, Nanfang Hospital, Southern Medical University)
Cai, Guan-Fu (Department of Gastroenterology Surgery, Guangdong General Hospital)
Yao, Xue-Qing (Department of Gastroenterology Surgery, Guangdong General Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.1, 2012 , pp. 377-381 More about this Journal
Abstract
The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.
Keywords
Phage peptide library; LoVo cells; subtraction biopanning; colorectal cancer; M13;
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