• 동의생리병리학회지
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• 제27권5호
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• pp.617-624
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• 2013

The purpose of this study is to investigate the protective effects of 25 herbal formulas on acetaminophen (APAP) or D-galactosamine (D-GalN)-induced hepatotoxicity in primary rat hepatocytes. Cell viability was measured using by Cell Counting Kit-8. 15 kinds of herbal formulas significantly reversed the cell viabilities of D-GalN-treated rat hepatocytes compared with D-GalN alone (p<0.05). In particular, 9 herbal formulas (Bangpungtongseong-san, Bojungikgi-tang, Galgeun-tang, Gumiganghwal-tang, Guibi-tang, Sagunja-tang, Samsoeum, Pyeongwi-san and Yijin-tang) showed the potent protective effects. However, 8 herbal formula exerted weak protective effects and 2 herbal formula did not exert effects on hepatotoxicity by D-GalN. On APAP-induced hepatotoxicity, 7 kinds of herbal formulas increased the viabilities of hepatocytes compare with APAP alone (p<0.05). These results could be provide a valuable information for the future in vivo or clinical studies to predict the hepatoprotective effects of herbal formulas.

• 대한본초학회지
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• 제30권6호
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• pp.7-15
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• 2015

Objectives : Rosa laevigata Michx. has been used for the treatment of renal disease in traditional Korean medicine. In this study, we investigated cytoprotective effect of R. laevigata water extract (RLE) against oxidative stress induced by arachidonic acid (AA) + iron.Methods : To evaluate the protective effects of RLE against AA + iron-induced oxidative stress in HepG2 cell, cell viability and changes on apoptosis-related proteins were assessed by MTT and immunoblot analyses. The effects of RLE on reduced glutathione level, production of reactive oxygen species and mitochondrial membrane potential were also monitored. Furthermore, to verify underlying molecular mechanism, NF-E2-related factor 2 (Nrf2) was examined by immunoblot analysis. Additionally, Nrf2 transactivation and its downstream target genes expression were also determined by reporter gene and realtime RT-PCR analyses.Results : RLE pretreatment (30-300 μg/ml) prevented cells from AA + iron-mediated cell death in a concentration dependent manner. In addition, 100 μg/ml RLE inhibited AA + iron-induced glutathione depletion, reactive oxygen species production and mitochondrial dysfunction. RLE accumulated nuclear Nrf2 and also transactivated Nrf2, which was evidenced by antioxidant response element- and glutathione S-transferase A2-driven luciferase activities and mRNA level of glutamate-cysteine ligase catalytic subunit, NAD(P)H:quinone oxidoreductase 1 and sestrin 2. Moreover, protective effect of RLE against AA + iron was abolished in Nrf2 knockout cells.Conclusions : These results indicate that RLE has the ability to protect hepatocyte against oxidative stress through Nrf2 activation.

• 대한본초학회지
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• 제38권5호
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• pp.1-7
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• 2023

Purpose : To compare the skin damage recovery efficacy of natural Angelicae Gigantis Radix extract (N-AGR) and cultivated A. Gigantis Radix extract (C-AGR). Through this, we confirmed whether the quality standards of herbal medicines recorded in the classic books make a difference in the experimental efficacy using epithelial cells. Methods : The quality standards of medicinal herbs in the classic books and the cultivation and processing conditions of two types of A. gigas were compared. After inducing oxidative stress with H₂O₂, cytoprotective property of N-AGR and C-AGR were evaluated through cell viability. Additionally, after wound formation of epithelial JB6 cells, N-AGR and C-AGR were treated to evaluate wound healing efficacy. Result : The natural A, gigas met the excellent quality standards of the classic books. N-AGR inhibited cell death by oxidative stress induced by H₂O₂, and was superior to C-AGR. Both N-AGR and C-AGR showed dose-dependent wound healing efficacy, but N-AGR was significantly superior to C-AGR. Conclusions : Through the oxidative stress of skin and skin wound healing efficacy experiments using epithelial cells, natural A. gigas showed superior efficacy compared to cultivated A. gigas.

• 대한한의학원전학회지
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• 제33권4호
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• pp.67-81
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• 2020

Objectives : This research aims to suggest a automatic data extract method for herbal formula combinations from medical classics' texts. Methods : This research was carried out by using Access of Microsoft Office 365 in Windows 10 of Microsoft. The subject text for extraction was 『Euijongsonik』. Using data sets of herb and dosage terminology, herbal medicinals and their dosages were extracted. Afterwards, using the position value of the character string, the formula combinations were automatically extracted. Results :The PC environment of this research was Intel Core i7-1065G7 CPU 1.30GHz, with 8GB of RAM and a Windows 10 64bit operation system. Out of 6,115 verses, 19,277 herb-dosage combinations were extracted. Conclusions : In this research, it was demonstrated that in the case of classical texts that are available as data, knowledge on herbal medicine could be extracted without human or material resources. This suggests an applicability of classical text knowledge to clinical practice.

• 한방비만학회지
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• 제3권1호
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• pp.1-6
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• 2003

Background: Recently, Obesity has become a social problem in all over the world including Korea. Dietary regulation, exercise and behavior modification therapy are used in the treatment of obesity, but until now, there have been many difficulties in treating it. Objectives: To examine in obese pre-menopausal Korean females the short-term efficacy for weight loss of an herbal formula (Slim-diet). Design: A 6-week longituidinal evaluation of a herbal formula (Slim-diet) in 35 pre-menopausal females (baseline age $31.6\;{\pm}\;6.1$) with body mass index > $25kg/m^2$. Body composition was measured using bioimpedence analysis (BIA) and dual energy X-ray absorptiometry (DXA). Anthropometry was done by same observer. Total cholesterol, total lipid, triglyceride, and LDL-cholesterol were measured. Results: Herbal formula (Slim-diet) treatment produced significant loss of weight (P < 0.001), percent of body fat (P < 0.001, both BIA and DXA) and waist circumference (P < 0.001). It also produced significant reduction of total cholesterol and total lipid levels (P < 0.05).

• 대한한의학방제학회지
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• 제27권1호
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• pp.87-100
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• 2019

Objective : This study's purpose was to review the clinical studies of Mahwang-tang (MHT). Method : We searched ten clinical studies of MHT using KISS, RISS, NAVER, PUBMED, Science Direct. Ten papers were sorted by 4 topics, as influenza, pediatric febrile symptoms, contact dermatitis, and adjuvant for chronic hepatitis C. At last we analyzed if these papers are consistent with the keywords of Shanghanlun provisions about MHT. Results : When summarizing these papers again by topic, there were 4 studies on influenza, 1 study on pediatric febrile symptoms, 1 study on contact dermatitis and 4 studies as an adjuvant for chronic hepatitis C. Conclusion : These studies were consistent with terms such as fever, headache, aversion to wind, body pain, pain in the lumbar, articular pain written in Shanghanlun provisions. In addition to this, research on anti-depressive effects of MHT is proceeding systematically. However, studies on nosebleeds and pulses are still underdeveloped and further studies are needed.

• 대한한의학방제학회지
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• 제11권2호
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• pp.29-40
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• 2003

Armeniacae Amarum Semen was well-known as a theraputic agent of asthma by flowing downward of qi and moistening of lung in Oriental Medicine. But there are many other effect in Armeniacae Amarum Semen such as, loosening the bowel to relieve constipation, removing stagnation of qi and food, dispersing accumulation of pathogen, parasiticide. We have been studying what the meaning of Armeniacae Amarum Semen in herbal formula and usage of Armeniacae Amarum Semen is.

• 대한한의학방제학회지
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• 제15권1호
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• pp.107-115
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• 2007

Objectives : The aim of this study is to analyze the prescription used for dysuria in herbal medication. Methods : Investigating the Oriental Medicine textbooks, I found the prescription used for dysuria. So I analyzed the herbal medication for frequency or compositon and classified it. Results : 1. The most used prescription on dysuria is Paljung-san. 2. The order of frequency of herb for treating dysuria is Moktong, Hwalseok, Gamcho, 3. The group of Yisusamseopyak is used for main prescription about 42%.

• 대한한의학방제학회지
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• 제23권2호
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• pp.209-224
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• 2015

Objective : The main purpose of this study is to find a reasonable solutions for utilization of herbal formulas based on the medical classics. Methods : We used official Ten 'Selected Korean Medical Classics(SKMC)'. For input of formulas from SKMC, we had to select proper editions of them. After consultations with experts of 'The Korean Medicine Society for the Herbal Formula Study' and 'Korean Society of Medical History', we chose the proper editions of SKMC, and according to those books, we built formulas database using microsoft office excel sheets, respectively. Results : After whole input procedures, we requested several experts to inspect the results and also, to correct errors in them. Input categories were name of formula, name of classic, classification, original source, composition herb, weight, unit of weight, weight conversion index, converted weight, basic formula, indications, reason for modification, preparing method, taking method and contraindications. Final number of whole formulas in DB was 32,882. Conclusions : We used SKMC and built database using excel program. Through this procedure, we can provide a developmental ways to make a foundation for herbal formulas from the medical classics, so that we can make herbal formulas be standardized or industrialized more efficiently.

• 대한한의학방제학회지
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• 제13권2호
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• pp.71-91
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• 2005

A/J mice injected with urethane(1mg/ g of body weight) develop tumors with distinct histological patterns, which are classified as solid and papillary. I divided the mice into 3 groups; control group treatment with saline, BYA group treatment with herbal formula, Bopheyangyoungjeon 0.3g/kg and BYB groups treatment with herbal formula 1.5g/kg. The administration of herbal medicine was done every day for 56 days. The experimental results from herbal medicine treatment were compared to those from a saline-treated control group. Serial sections of the whole lung(150 to 200 sections per mouse) showed solid and papillary tumors arose from the pulmonary acinus, invading the bronchioles only as the tumors grew. The number of tumor, in the 8-weeks groups, are decreased in the experimental groups compared to control group. COX-2 protein and IGF- I protein expression was more increased in lung tumors of the control group compared to BYA and BYB groups. These results suggest that Bopheyangyoungjeon extract suppress the carcinogenesis of lung in the A/J mouse.