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Hepatoprotective Effects of 25 Herbal Formulas in Primary Rat Hepatocytes  

Jin, Seong Eun (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Jeong, Soo-Jin (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Ha, Hyekyung (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.5, 2013 , pp. 617-624 More about this Journal
Abstract
The purpose of this study is to investigate the protective effects of 25 herbal formulas on acetaminophen (APAP) or D-galactosamine (D-GalN)-induced hepatotoxicity in primary rat hepatocytes. Cell viability was measured using by Cell Counting Kit-8. 15 kinds of herbal formulas significantly reversed the cell viabilities of D-GalN-treated rat hepatocytes compared with D-GalN alone (p<0.05). In particular, 9 herbal formulas (Bangpungtongseong-san, Bojungikgi-tang, Galgeun-tang, Gumiganghwal-tang, Guibi-tang, Sagunja-tang, Samsoeum, Pyeongwi-san and Yijin-tang) showed the potent protective effects. However, 8 herbal formula exerted weak protective effects and 2 herbal formula did not exert effects on hepatotoxicity by D-GalN. On APAP-induced hepatotoxicity, 7 kinds of herbal formulas increased the viabilities of hepatocytes compare with APAP alone (p<0.05). These results could be provide a valuable information for the future in vivo or clinical studies to predict the hepatoprotective effects of herbal formulas.
Keywords
Herbal formula; Hepatoprotection; Primary rat hepatocytes; Cell viability;
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Times Cited By KSCI : 3  (Citation Analysis)
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