• Korean Journal of Microbiology
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• v.3 no.1
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• pp.1-6
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• 1965

Over a period of 1962-64, a transmission-experiment of witchess' broom of jujube tree by stem-grafting was conducted. When stem-grafting of sound scions upon diseased roots or diseased scions upon sound roots were carried out, disease transmission of high rate was witnessed; 99% in the former and, in the latter, 62% of the stocks which saw union by callussing and had new shoots. Even when the diseased scions by stem-grafting or the diseased buds by budding upon sound stock died away, the transmission rate was 21% in stem-grafting and 14% in budding which seems to show that, when tissues of diseased plants and stocks are kept contacted over a certain period, the disease transmission occurs. And when the recovered scions taken from once diseased tree were grafted upon diseased roots, the transmission rate was 100 % and therefore it is presumed that the immunity could not be acquired even under the assumption of complete recovery from the disease. In stem-grafting of the diseased scions upon sound roots, 98% of the scions which were stored in the cellar, overwintered and grafted in spring was diseased, whereas the disease rate of the scions which were cut and grafted in spring was only 33%. It was particularly noteworthy that 90% of the scions in the former case and only 3% in the latter case were diseased as of June 18th approximately 2 months after the actual grafting and then the latter advanced to 33% with the passage of time. It appears that the pathogen in branches and shoots of the diseased trees standing outdoors become inactivated or diminished during winter. Through its symptom, pathological change in tissue, and easy transmission of the disease via stem-grafting, it seems certain that the pathogen of the witches broom disease in jujube tree is a virus.

• Journal of the Korean Wood Science and Technology
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• v.34 no.6
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• pp.1-11
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• 2006

Grafted tissues were investigated using various microscopic techniques. Pinus thunbergii was used as stock and scion and autografted by cleft graft method. Histochemically, grafting processes can be proceeded by four stages: 1) formation of necrotic layer, 2) proliferation of callus, 3) development of neo-cambium from callus, and 4) restoration of new vascular xylem. Necrotic la yer composed of pectin and lignin was gradually degraded during grafting process and disappeared when new union was formed between stock and scion. A large number of starch and lipid bodies in the cytoplasm were also gradually degraded during grafting process and disappeared at the grafting interface. Nucleus and plasmodesmata were not changed. Bubble-like callus was generated from all living parenchyma cells and from the callus. The tracheary elements differentiated from the callus had either reticulate or pit-like thickenings in the secondary walls with bordered pits. Secondary cell wall thickening occurred toward filing to the void parts between reticulated secondary wall. Tracheids formed in the secondary xylem were short with irregular wall thickness. New secondary xylem cells with swirled shapes, which developed in graft union were oriented horizontally and obliquely to axis of the stem.

• Journal of Plant Biology
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• v.11 no.1
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• pp.27-32
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• 1968

In the present experiment as a basic study for establishing a hybrid seed orchard, an observation was made on the survivability of grafts using Pinus rigida as the stock and its hybrids as the sciion, and on the shape and germination of pollen collected from grafted plants. The results are summarized as follows: 1. The hybrid scion of P. rigida.taeda or $P. rigida{\times}(P. rigida.taeda)$ grafted into P. rigida stock showed lower survivability than the homoplastic graftings of P. rigida. 2. Five among the eight eleven-year old scions homoplastically grafted into two-year old stock for the staminate strobile one year after their grafting. 3. There was no difference in the size and germination ability between the pollen collected from homoplastically grafted plant and from the normal one. 4. The pollen collected from the grafted plant is thought to be usable for the control pollination as the germination of such pollen was considerably good.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.78-84
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• 2005

Charactertistics of growth from graft induced three stock of red pepper cultivar were analysed. R-safe rootstock was more higher and vigorous than that of the Yeok kang, Konesian hot cultivar at seedling stage and had good characteristics for grafting in the space of cut surface and the amount of sap released. Numbers of branches were more numerous in the grafted plants than those of non-grafted as grafting affected their growths in the process of branching. There was no distinct difference in plant height among the different rootstock. However the R-safe rootstock showed considerably high growth in the 41st days after grafting. Grafting was effective in the early flowering and the R-safe was the earliest in flowering because of it's good growth under the low temperature.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.73.1-73
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• 2003

In order to cultivate watermelon on farm, grafting of the watermelon seedling to the watermelon stock is necessary because the watermelon root is less viable than the root of watermelon stock. Recently, commercially important watermelon varieties further require a resistant stock against especially CGMMV to control the heavy loss of the total yield of watermelon by CGMMV infection. Therefore, we have set out a project to develop a CGNEMV-resistant watermelon stock. We have successfully transformed dozens of watermelon stocks (gongdae) during last two years especially using a cDNA encoding the coat protein of CGMMV (cucumber green mottle mosaic virus). Recently we have tested levels of resistance of those watermelon stocks against CGMMV infection. For CGMMV inoculation, the leaves of one month old gongdae (T1) were rubbed by carborundum mixed with the CGMMV. A total of 140 plants (T1) were exposed to the CGMMV and we found that ten plants were completely resistant to virus infection. This is the first report that by genetic engineering a cucubitaceae crop resistant to CGMMV infection is ever developed. Further information will be provided in the poster.

• Journal of Korean Society of Forest Science
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• v.40 no.1
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• pp.51-56
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• 1978

In the present study an experiment on the interspecific grafting of European and American filberst on the root stock of Korean filbert (C. heteropylla & C. sieboldiana) by means of veneer graftings in the green house was carried out and those survival percentage and growth was also investigated. The obtained results can be summarized as follows. 1. Interspecific grafting of European and American filberts on root stock of Korean filbert (C. heterophylla & C. sieboldiana) was 97%~98.3% of survival. 2. Those Interspecific grafts was higher compatibilities and nonsukering of root stocks of Korean filberts in the 1~2 years old. 3. Those mean growth of interspecific grafts was 106.7cm~111cm of height and 11.7mm~14.6mm of diameters at the stem base.

• Journal of Sericultural and Entomological Science
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• v.5
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• pp.1-5
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• 1965

The purpose of this work is to investigate the effects of picking leaves in autumn on the non-budding and survival of grafting. The results are as follows. 1) The leaf picking in autumn causes an increase of the non-budding ratio in the following spring. When tue whole leaves picking is compared with the control, the non-budding ratio increases 46 percent. In case of tone leaves on a single stem are picked in various tray, the non-budding ratio of picked portion is higher than that of non-picked. 2) The leaf picking in autumn with winter bud being grafted on stock in the next spring, decrease the survival of grafting. In case some leaves on a single stem are picked in various way, the winter bud of picked part is always poor in survival. 3) In the event of leavf picking in autumn, the yield always decreases 7∼20 percent, especially this phenomenon becomes serious in the under half part of the stem(20%) and whole leaves picking(12％) treatments.

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.68-75
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• 1982

This study was carried out to promote percent survival of the walnut seedling grafting. The hardwood scions of the walnut were grafted on the nures seed-stock of the Juglans mandshurica Mat in an electric heating bed, then planted in field. The results obtained from the study were as follows : The optimum time of scion cpllection was from January to February. The best medium of the seed bed was sandy soil. The best grafting time was form the early to the 20the of the march. When the grafted seedling in the heating bed was trans-planted on filed 90percent of the seedlings was survived until autmn. The percent grafting on the elective heating bed was 90%. Crown gall occuring frequently in chestnut nurse graft was not appeared in juglans mandshurica Max grafted seedling of after outplanting. The grafted seedlings have not shown any physiological defects but developed normaly 3 years since grafting.

• Korean journal of applied entomology
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• v.3
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• pp.1-5
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• 1964

Symptom of the mosaic disease of black locust which is presently widespread in Korea was observed and transmission of the disease was investigated by means of grafting Since its symptom was typically mosaic and its transmission was easily accomplished, the disease was considered to be caused by a virus. In investigation of transmission by grafting, it was found out that the discase was easily transmitted in both of the following cases, i. e., healthy seedlings grafted with diseased scions and diseased roots with healthy scions. In the healthy seedlings plus diseased scions grafting, some grafted seedlings were purposedly deprived of the diseased scions after, a certain length of time(10∼30 days), while some were left grafted. The former case showed much higher transmission rate than the latter case, but no fluctuation in rate of transmission by the length of contact period(10-30 days) was recognized. And even when the union between stock and diseased scion by callusing was not successful, the transmission was withnesed. On the basis of the symptom, transmission by grafting, and possibility of transmission by sap inoculation(not published yet), it is supposed that the mosaic virus belongs to the group of the black locust mosaic virus that has been reported in southeastern Europe by Atanasoff(1935) and Milinko et al(1961). It is very likely that the witches' broom of black locust recorded by Myung-O Kim et al(1961) in Korea is the mosaic disease.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.45-50
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• 2005

Virus-free stocks was produced by the combination of the heat treatment of virus infected plant and shoot-tip grafting (STS). To produce virus-free stocks, the plants infected with citrus viruses were used for virus-free stock production using the modified method of STG in thermotherapy at $40^{\circ}C$ for 16 hours in the light, and at $30^{\circ}C$ for 8 hours of darkness for 4 weeks. Trifoliate orange (P. trifoliata) were used as rootstock seedling for STG. Percentages of virus-free stocks against citrus tristeza virus (CTV), satsuma dwarf virus (SDV) and citrus tatter leaf virus (CTLV) were $75.7\%,\;100.0,\%\;82.6\%$ respectively. Shoot tip size for successful STG were as small as possible. Less than $0.3\;\cal{mm}$ of shoot tips gave the hight efficiency of virus free plants but survival rates were low. And, survival rate after shoot-tip culture was analyzed and the rates were dependant on the cultivars; Yuzu cultivar showed the hight survival rate ($74.6\%$) and early satsuma mandarin (Iwasagi) was $13.3\%$ as the lowest cultivar. But citrus trees were not succeed to grown, turned brown, and died.