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Histochemical and Cytochemical Studies on the Secondary Xylem Formation during Grafting Process of Pinus thunbergii  

Kim, Jong Sik (Department of Forest Products and Technology, College of Agriculture, Chonnam National University)
Lee, Kwang Ho (Department of Forest Products and Technology, College of Agriculture, Chonnam National University)
Kim, Yoon Soo (Department of Forest Products and Technology, College of Agriculture, Chonnam National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.34, no.6, 2006 , pp. 1-11 More about this Journal
Abstract
Grafted tissues were investigated using various microscopic techniques. Pinus thunbergii was used as stock and scion and autografted by cleft graft method. Histochemically, grafting processes can be proceeded by four stages: 1) formation of necrotic layer, 2) proliferation of callus, 3) development of neo-cambium from callus, and 4) restoration of new vascular xylem. Necrotic la yer composed of pectin and lignin was gradually degraded during grafting process and disappeared when new union was formed between stock and scion. A large number of starch and lipid bodies in the cytoplasm were also gradually degraded during grafting process and disappeared at the grafting interface. Nucleus and plasmodesmata were not changed. Bubble-like callus was generated from all living parenchyma cells and from the callus. The tracheary elements differentiated from the callus had either reticulate or pit-like thickenings in the secondary walls with bordered pits. Secondary cell wall thickening occurred toward filing to the void parts between reticulated secondary wall. Tracheids formed in the secondary xylem were short with irregular wall thickness. New secondary xylem cells with swirled shapes, which developed in graft union were oriented horizontally and obliquely to axis of the stem.
Keywords
grafting process; necrotic layer; callus; starch; lipid bodies; neo-cambium; secondar y xylem; tracheary elements; secondary cell wall formation;
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