• Journal of Gastric Cancer
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• 제5권3호
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• pp.191-199
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• 2005

목적: Helicobacter pylori(H. pylori) 감염이 숙주의 혈관형성을 유도하는 작용은 잘 알려져 있으나, 이러한 혈관형성 유도작용을 억제하는 기전이나 치료법은 아직 연구되지 않았다. 이에 저자들은 HP 감염이 위 점막의 혈관형성에 미치는 영향과 이에 대한 gastric proton pump inhibitor (PPI)의 강력한 억제 효과를 규명하기 위해 본 연구를 시행하였다. 대상 및 방법: H. pylori 양성인 20명의 위염 환자들과 음성인 18명의 위염 환자들을 대상으로 내시경생검으로 수집한 위 점막의 CD34의 발현을 조사하였다. Hypoxia-inducible factor-1 alpha $(HIF-1{\alpha})$와 vascular endothelial growth factor(VEGF)의 발현은 RT-PCR로 측정하였다. H. pylori 감염이 human umbilical vein endothelial cell의 분화에 미치는 영향을 평가하기 위하여 in vitro 혈관형성 실험을 시행하였다. 결과: 면역조직화학 염색 검사상 H. pylori음성 위염에서 보다 H. pylori양성 위염에서 위 점막층의 CD34양성 혈과이 유의하게 많이 발현되었으며, 이러한 결과는 $HIF-1{\alpha}$의 발현과 밀접한 상관관계를 보였다. H. pylori에 감염된 위 점막 세포의 조건화된 배지는 human umbilical vein endothelial cell의 세관 형성을 자극하였다. 그리고 H. pylori 박멸치료에 사용되는 PPI가 HP 유도성 혈관형성을 현저히 억제하는 효과를 발견하였고, PPI의 작용은 H. pylori 유도성 혈관형성의 주된 신호전달 경로인 MAP kinase ERK1/2의 인산화를 억제함으로 이루어졌다. 결론: PPI가 H. pylori 유도성 혈관형성을 억제하다: 결과는 H, pylori가 관련된 암 발생의 예방을 위하여 PPI를 사용한 항혈관형성 치료가 사용될 수 있음을 제시한다.

• 한국축산식품학회지
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• 제28권5호
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• pp.660-666
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• 2008

본 연구에서는 식용유지로 사용되는 돈지, 대두유 및 고추씨유를 $100^{\circ}C$에서 3일간 저장하면서 열안정성을 비교하고, 캡사이신의 첨가가 대두유 및 돈지의 산화안정성 효과에 대하여 실험하였다. 그 결과 돈지>대두유>고추씨유의 순서로 고추씨유가 가장 열에 안정적임을 밝혔다. 가장 열에 안정한 고추씨유를 돈지와 대두유에 혼합했을 때의 저장기간에 따른 산화 정도를 비교하였는데, 돈지에서는 고추씨유 30% 이상에서 산화가 억제되지만, 대두유에서는 그보다 많은 60% 이상이 혼합되어야 산화가 억제되었다. 또한 고추씨유의 주요 성분인 캡사이신을 고추씨유에 함유된 양만큼을 대두유와 돈지에 각각 첨가하여 열처리 했을 때 산화안정성에 미치는 영향도 검토하였다. 그 결과 1,200 ppm과 2,400 ppm의 캡사이신, 또는 0.3% 알파-토코페롤을 대두유에 첨가하였을 때 캡사이신은 대두유의 산화에 아무런 영향을 미치지 못하였고 알파-토코페를은 오히려 산화를 촉진시켰다. 하지만 돈지에 동량의 캡사이신이 첨가되었을 때에는 산화를 효과적으로 억제하였다. 이러한 결과로부터 고추씨유 또는 캡사이신은 돈지를 소재로 하는 튀김유나 분말유지의 가공에 이용하면 매우 좋은 산화억제 효과를 얻을 것으로 사료된다.

• 한국식품저장유통학회지
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• 제20권5호
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• pp.684-690
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• 2013

이 연구의 목적은 소리쟁이 뿌리로부터 얻어진 다양한 용매 추출물의 항산화 활성과 광 피해 억제 효과를 확인하는 것이다. 항산화 활성은 총 페놀 함량 분석과 DPPH 라디칼 소거능, ABTS 라디칼 소거능, 단일항산소 억제 효과로 확인하였다. DPPH 라디칼 소거 활성을 나타내는 $IC_{50}$ 값은 0.005~0.093 mg/mL를 나타내어 소리쟁이 뿌리 추출물의 DPPH 소거 활성이 우수하다는 것을 알 수 있었다. 특히 에틸아세테이트 추출물이 0.005 mg/mL로 높은 DPPH 라디칼 소거 활성을 나타내었는데, 비교 물질로 주로 사용되는 ascorbic acid의 0.010 mg/mL 보다 높은 우수한 항산화력을 가지는 것을 확인하였다. 단일항산소 억제 효과를 나타내는 $QC_{50}$ 값은 부탄올과 에틸아세테이트 추출물의 경우 각각 0.464, 0.365 mg/mL을 나타내어 높은 소거 활성을 보였으며, 대장균 및 DNA와 같은 생체기관의 피해로부터 이들 추출물의 광 피해 억제 효과를 시험한 결과 강한 빛에 의해 야기되는 활성산소의 피해로부터 생물체를 보호해주는 것을 알 수 있었다. 총 페놀 함량도 에틸아세테이트와 부탄올 추출물에서 높은 수준을 나타내었다. 결론적으로 소리쟁이 뿌리의 추출물은 생물체의 산화적 스트레스로부터 야기되는 활성산소를 억제하는 중요한 역할을 하며, 보다 심도 깊은 연구를 진행한다면 새로운 생물 소재로 활용성이 높을 것으로 사료된다.

• 대한한의학방제학회지
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• 제15권1호
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• pp.213-228
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• 2007

Objectives : The purpose of this report was to investigate the chemotherapeutic effect of Bujeonghangamtang against cancer cells. Materials and Methods : Various cancer cell lines including PANC-1, C6 glioma, SH-SY5Y, HepG2, and MCF-7 cells, were used. Apoptosis was determined by DAPI nuclei staining and flow cytometry in PANC-1 cells treated with 1 mg/ml Bujeonghangamtang for 48 hr. Expression of cell cycle arrest mediators including, cdc2p34 and cyclin B1 proteins were measured by Western blot analysis. Mitochondrial membrane potential was measured by fluorescence staining with JC-1, rhodamine 123. Result : Bujeonghangamtang induced the apoptosis of PANC-1, which was characterized as nucleic acid and genomic DNA fragmentation, chromatin condensation, and sub-G0/G1 fraction of cell cycle increase. but not C6 glioma, SH-SY5Y, HepG2, and MCF-7 cells. PANC-1 cells were markedly sensitive to Bujeonghangamtang. Treatment with Bujeonghangamtang resulted in the decreased expression of cdc2p34 and cyclin B1. Treatment with Bujeonghangamtang also increased the ROS production and induced mitochondrial dysfunction. Conclusion : Bujeonghangamtang exerted cytotoxicity against human Pancreatic cancer cells via cell cycle arrest-mediated apoptotic signaling including ROS production and mitochondrial dysfunction. Our data suggest that Bujeonghangamtang may be an important modulator of chemosensitivity of cancer cells against anticancer chemotherapeutic agents.

• 식물병연구
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• 제18권4호
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• pp.268-276
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• 2012

Broad bean wilt virus 2 (BBWV2), genus Fabavirus, subfamily Comovirinae, family Secoviridae, causes damage in many economically important horticultural and ornamental crops. Sequence alignments showed several conserved sequences in 5' non-coding regions (5' NCRs) of RNA 1 and RNA 2 in all BBWV2 strains characterized so far. Based on this observation, we generated a hpRNA construct (pIR-BBWV2) harboring an inverted repeat containing a 210 bp cDNA fragment homologous to 5' NCR portion of BBWV2 RNA 1 to investigate the silencing potential for its ability to interfere with a rapidly replicating BBWV2. Agrobacterium-mediated transient expression of the IR-BBWV2 had a detrimental effect on BBWV2 infection, showing no distinct symptoms in non-inoculated leaves of the agroinfiltrated Nicotiana benthamiana plants. BBWV2 genomic RNAs were not detected by RT-PCR from tissues of both the inoculated leaves and upper leaves of the agroinfiltrated plants. Accumulation of virus-derived small interfering RNAs was detected in the inoculated leaf tissues of N. benthamiana plants elicited by transient expression of IR-BBWV2 indicating that RNA silencing is responsible for the resistance to BBWV2.

• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.417-426
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• 2020

Bacillus amyloliquefaciens is an important plant disease-preventing and growth-promoting microorganism. B. amyloliquefaciens WS-8 can stimulate plant growth and has strong antifungal properties. In this study, we sequenced the complete genome of B. amyloliquefaciens WS-8 by Pacific Biosciences RSII (PacBio) Single Molecule Real-Time (SMRT) sequencing. The genome consists of one chromosome (3,929,787 bp) and no additional plasmids. The main bacteriostatic substances were determined by genome, transcriptome, and mass spectrometry data. We thereby laid a theoretical foundation for the utilization of the strain. By genomic analysis, we identified 19 putative biosynthetic gene clusters for secondary metabolites, most of which are potentially involved in the biosynthesis of numerous bioactive metabolites, including difficidin, fengycin, and surfactin. Furthermore, a potential class II lanthipeptide biosynthetic gene cluster and genes that are involved in auxin biosynthesis were found. Through the analysis of transcriptome data, we found that the key bacteriostatic genes, as predicted in the genome, exhibited different levels of mRNA expression. Through metabolite isolation, purification, and exposure experiments, we found that a variety of metabolites of WS-8 exert an inhibitory effect on the necrotrophic fungus Botrytis cinerea, which causes gray mold; by mass spectrometry, we found that the main substances are mainly iturins and fengycins. Therefore, this strain has the potential to be utilized as an antifungal agent in agriculture.

• The Korean Journal of Physiology and Pharmacology
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• 제7권4호
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• pp.217-221
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• 2003

To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-$17{\beta}$ on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-$17{\beta}$ did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin, cholecystokinin (CCK), and acetylcholine markedly stimulated pancreatic fluid and amylase secretion. Progesterone initially enhanced secretin-induced amylase secretion, but this stimulatory response declined thereafter to basal value. Moreover, secretin-induced fluid secretion was not affected by infusion of progesterone. Therefore, initial increase of secretion-induced amylase secretion by progesterone seems to be a non-specific action by washout effect of secretin. Estradiol-$17{\beta}$ failed to change the secretin-induced fluid and amylase secretion. Both progesterone and estradiol-$17{\beta}$ did not exert any influence on CCK-induced fluid and amylase secretion. Acetylcholine-induced exocrine secretion of isolated perfused pancreas also was not affected by intra-arterial infusion of progesterone or estradiol-$17{\beta}$. It is concluded from the above results that progesterone could enhance the spontaneous pancreatic fluid and amylase secretion of isolated perfused rat pancreas through non-genomic shortterm action, and that these effects could be masked by more potent stimulants such as secretin, CCK, and acetylcholine.

• Asian-Australasian Journal of Animal Sciences
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• 제28권2호
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• pp.166-170
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• 2015

Foot-and-mouth disease (FMD) is a highly contagious disease affecting cloven-hoofed animals and causes severe economic loss and devastating effect on international trade of animal or animal products. Since FMD outbreaks have recently occurred in some Asian countries, it is important to understand the relationship between diverse immunogenomic structures of host animals and the immunity to foot-and-mouth disease virus (FMDV). We performed genome wide association study based on high-density bovine single nucleotide polymorphism (SNP) chip for identifying FMD resistant loci in Holstein cattle. Among 624532 SNP after quality control, we found that 11 SNPs on 3 chromosomes (chr17, 22, and 15) were significantly associated with the trait at the p.adjust <0.05 after PERMORY test. Most significantly associated SNPs were located on chromosome 17, around the genes Myosin XVIIIB and Seizure related 6 homolog (mouse)-like, which were associated with lung cancer. Based on the known function of the genes nearby the significant SNPs, the FMD resistant animals might have ability to improve their innate immune response to FMDV infection.

• 한국수산과학회지
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• 제44권4호
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• pp.352-358
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• 2011

The presence of ISKNV-like viruses in various freshwater ornamental fish species imported from Asia was confirmed by polymerase chain reaction(PCR) amplification of the ATPase(adenosine triphosphatase) gene. Interestingly, molecular analyses of the Open Reading Frame 25(ORF25) region of these isolates based on the ISKNV(Infectious spleen and kidney necrosis virus) genome revealed the presence of various repetitive sequences. ORF25 repeat sequence length had no effect on cumulative mortality of rock bream Oplegnathus fasciatus challenged with tissue homogenates of infected pearl gourami, Trichogaster leeri; silver gourami, Trichogaster microlepis; blue gourami, or Trichogaster trichopterus. All isolates induce cumulative mortalities after 12 days of infection, confirming that ORF25 polymorphism did not affect the pathogenicity of ornamental fish megalocytiviruses that cross infect rock bream, a seawater fish. Also, no statistically significant differences in spleen index or viral copy number in infected tissues was detected between isolates with varying ORF25 repeat sequence lengths. However, further studies are necessary to fully characterize the functional characteristics of these polymorphisms in megalocytivirus disease in ornamental fishes.

• Ocean and Polar Research
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• 제38권3호
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• pp.185-193
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• 2016

본 연구에서는 북극 육상식물인 R. hyperboreus를 유기용매로 추출하여 얻어진 추출물에 대하여 세포내 ROS와 peroxynitrite 소거활성을 측정하였다. 추출물의 유의적인 소거활성을 확인한 후에 구성성분의 극성에 따른 소거효과의 변화를 보기 위하여 용매분획을 실시하여 n-hexane, 85% aq.MeOH, n-BuOH, water 분획물을 얻었다. 각 용매분획의 폴리페놀 함량을 측정하였으며 그 결과 n-hexane, water, 85% aq.MeOH, n-BuOH 분획의 순서대로 폴리페놀 함량이 증가하였다. 각 용매분획에 대한 항산화 활성을 검색한 결과 n-hexane 분획의 경우에는 세포생존율에 기인한 어느 정도의 활성변화는 있었으나 대체적으로 소거 활성은 폴리페놀 함량에 비례하였다. 폴리페놀 함량이 가장 높은 n-BuOH 분획물이 가장 좋은 항산화활성을 나타내었으며 그 다음 번째로 폴리페놀 함량이 높은 85% aq.MeOH 분획물이 두 번째로 좋은 항산화활성을 나타내었다.