Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Transient Expression of Homologous Hairpin RNA Interferes with Broad bean wilt virus 2 Infection in Nicotiana benthamiana

  • Yoon, Ju-Yeon (PVGB, Department of Horticulture and Landscape, College of Natural Science, Seoul Women's University) ;
  • Ryu, Ki Hyun (PVGB, Department of Horticulture and Landscape, College of Natural Science, Seoul Women's University) ;
  • Choi, Seung-Kook (Horticultural & Herbal Environmental Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Gug Sun (Horticultural & Herbal Environmental Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kwon, Soon Bae (Ganwon Agricultural Research and Extension Services) ;
  • Park, Jin Woo (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Palukaitis, Peter (PVGB, Department of Horticulture and Landscape, College of Natural Science, Seoul Women's University)
  • Received : 2012.10.30
  • Accepted : 2012.11.26
  • Published : 2012.12.31
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Abstract

Broad bean wilt virus 2 (BBWV2), genus Fabavirus, subfamily Comovirinae, family Secoviridae, causes damage in many economically important horticultural and ornamental crops. Sequence alignments showed several conserved sequences in 5' non-coding regions (5' NCRs) of RNA 1 and RNA 2 in all BBWV2 strains characterized so far. Based on this observation, we generated a hpRNA construct (pIR-BBWV2) harboring an inverted repeat containing a 210 bp cDNA fragment homologous to 5' NCR portion of BBWV2 RNA 1 to investigate the silencing potential for its ability to interfere with a rapidly replicating BBWV2. Agrobacterium-mediated transient expression of the IR-BBWV2 had a detrimental effect on BBWV2 infection, showing no distinct symptoms in non-inoculated leaves of the agroinfiltrated Nicotiana benthamiana plants. BBWV2 genomic RNAs were not detected by RT-PCR from tissues of both the inoculated leaves and upper leaves of the agroinfiltrated plants. Accumulation of virus-derived small interfering RNAs was detected in the inoculated leaf tissues of N. benthamiana plants elicited by transient expression of IR-BBWV2 indicating that RNA silencing is responsible for the resistance to BBWV2.

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References

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