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http://dx.doi.org/10.4217/OPR.2016.38.3.185

Antioxidant Capacity of Crude Extract and Its Solvent Fractions of Arctic Terrestrial Plant Ranunculus heperporeus  

Lee, Jung Im (Division of Marine Bioscience, Korea Maritime and Ocean University)
Kim, Hojun (Division of Marine Bioscience, Korea Maritime and Ocean University)
Seo, Hyo-Won (National Institute of Highland Agriculture, RDA)
Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences Silla University)
Seo, Youngwan (Division of Marine Bioscience, Korea Maritime and Ocean University)
Publication Information
Ocean and Polar Research / v.38, no.3, 2016 , pp. 185-193 More about this Journal
Abstract
In this study, antioxidative potentials of the crude extract and its four solvent fractions from the Arctic terrestrial plant Ranunculus heperporeus were evaluated by using four different activity tests, including the inhibition of intracellular reactive oxygen species (ROS) and lipid peroxidation in Raw 264.7 cells as well as determining the extent of both the scavenging of peroxynitrite ($ONOO^-$) and the oxidative damage of genomic DNA purified from Raw 264.7 cells. Based on a comparative analysis, n-BuOH, and 85% aq.MeOH solvent fractions showed good scavenging effects on the production of intracellcular ROS and inhibited membrane lipid peroxidation and DNA oxidation. In addition, n-BuOH and 85% aq.MeOH fractions exhibited good scavenging effects on both authentic peroxynitrite and one generated from SIN-1. Among the samples tested, the n-BuOH fraction revealed the strongest antioxidant effect.
Keywords
Ranunculus heperporeus; the Arctic terrestrial plant; antioxidant; reactive oxygen species;
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Times Cited By KSCI : 5  (Citation Analysis)
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