• KSBB Journal
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• 제10권1호
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• pp.38-45
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• 1995

Aspergillus oryzae에서 A. nidulans의 glyceral d dehyde-3-phosphate dehydrogenase (gpdA)와 trpC, prmoter의 발현 능력 을 E. coli lacZ gene fusion을 이용하여 비교.분석하였다. A. oryzae 내에서 발현된 E. coli $\beta$galactosidase의 specific activIty를 조사하여 본 결과, gpdA promoter를 가지는 transformant들 에서는 2,000unit/ug of protem 정도의 activity를 보이는 반면, trpC, promater를 가지고 있는 transformant들에서는 10.5~52.3unit/ug of protein 정도의 activity를 보였다. 이 결과로부터 A. oryzae 내에서 A. nidulans의 gpdA promoter가 trpC, promoter에 비해 70 배 정도 더 강한 발현 능력을 보이고 있음을 알 수 있다. Western blot 분석에서도 gpdA promoter를 가지고 있는 transf ormant에서 더 많은 E. coli $\beta$-galactosidase가 발현된 것 을 보여 주고 있다. 또한 southern blot 분석에서는 이러한 강한 발현이 transform된 plasmid의 copy number와 상관 없음을 보여주고 있다.

• Bulletin of the Korean Chemical Society
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• 제33권5호
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• pp.1451-1456
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• 2012

A novel tetrathiafulvalene (TTF)-based main-chain polymer (6TTF-polymer) was successfully synthesized via a condensation polymerization between a newly synthesized dihydroxy TTF derivative and a malonyl chloride, and its chemical structure was characterized by spectroscopic techniques. Molecular weight of the 6TTF-polymer (9,030 g/mol by gel permeation chromatography) was large enough to form the ductile film. The electrochemical and optical properties of the 6TTF-polymer were further estimated by cyclic voltammetry, ultraviolet and photoluminescence spectroscopes. The highest occupied molecular orbital level ($E_{HOMO}$=-4.79 eV) and band-gap energy ($E_g$=1.91 eV) of the 6TTF-polymer suggested that TTF-based polymer could act as a good electron donating material for the optoelectronic applications.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권8호
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• pp.3791-3805
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• 2016

The visual attention mechanism includes 2 attention models, the bottom-up (B-U) and the top-down (T-D), the physiology of which have not yet been accurately described. In this paper, the visual attention mechanism is regarded as a Bayesian fusion process, and a visual attention model based on particle filter is proposed. Under certain particular assumed conditions, a calculation formula of Bayesian posterior probability is deduced. The visual attention fusion process based on the particle filter is realized through importance sampling, particle weight updating, and resampling, and visual attention is finally determined by the particle distribution state. The test results of multigroup images show that the calculation result of this model has better subjective and objective effects than that of other models.

• 대한치과교정학회지
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• 제11권2호
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• pp.57-64
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• 1981

The purpose of this study was to investigate the effect of hydrocortisone in the fusion mechanism of the secondary palatine shelves in the rat. Pregnant rats were injected with 5.0 mg/100 gm body weight of hydrocortisone for 3 days between the 7th day and the 9th day of pregnancy. Between the 14th day md the 18 day of pregnancy, the fetuses were removed and decapitated to be immersed in $10\%$ formalin and Carney's solution. Preparations were stained with alizarin red S, hematoxylin-eosin and alcian blue respectively, and partly were treated for Periodic acid-Schiff reaction. The results were as followings: 1. The incomplete fusion of the palatal epithelium took place though the polarity of epithelial cells. 2. At the edge of shelves the differentiation of mesenchymal cells was observed, but the inter penetration of mesenchyme was not shown. 3. It was considered that the phenomenon of hypocalcification in matrix had relation to the decrease stainability to alizarin red S in shelves. 4. It might be concluded that the connective tissue under epithelium showed the decrease tendency to alcian blue and PAS reaction due to the inhibition of the synthesis of the mucopolysaccharide.

• BMB Reports
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• 제34권4호
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• pp.371-378
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• 2001

The Glycoprotein D (gD) gene of the HSV-1 strain F was cloned, sequenced, recombinated into the HcNPV (Hyphantria cunea nuclear polyhedrosis virus) expression vector and expressed in insect cells. The gD gene was located in the 6.43 kb BamHI fragment of the strainF. The open reading frame (ORF) of the gD gene was 1,185 by and codes 394 amino acid residues. Recombinant baculoviruses, GD-HcNPVs, expressing the gD protein were constructed. Spodoptera frugiperda cells, infected with the recombinant virus, synthesized a matured gX-gD fusion protein with an approximate molecular weight of 54 kDa and secreted the gD proteins into the culture media by an immunoprecipitation assay The fusion gD protein was localized on the membrane of the insect cells, seen by using an immunofluorescence assay The deduced amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. These results indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins.

• Carbon letters
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• 제15권4호
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• pp.290-294
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• 2014

New precursors, poly(acrylonitrile-co-crotonic acid) (poly(AN-CA)) and poly(acrylonitrile-co-itaconic acid-co-crotonic acid) (poly(AN-IA-CA)) copolymers, for the preparation of carbon fibers, were explored in this study. The effects of comonomers with acidic groups, such as crotonic acid (CA) and/or itaconic acid (IA), on the stabilization of nanofibrous polyacrylonitrile (PAN) copolymers were studied. The extent of stabilization, evaluated by Fourier transform infrared spectroscopy, revealed that the CA comonomer could retard/control the stabilization rate of PAN, in contrast to the IA comonomer, which accelerated the stabilization process. Moreover, the synthesized PAN copolymers containing CA possessed higher Mv than those of the IA copolymers and also showed outstanding dimension stability of nanofibers during the stabilization, which may be a useful property for improving the dimensional stability of polymer composites during manufacturing.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.394-399
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• 2002

In this paper, Multisource data classification methods based on Bayesian formula are considered. For this decision fusion scheme, the individual data sources are handled separately by statistical classification algorithms and then Bayesian fusion method is applied to integrate from the available data sources. This method includes the combination of each expert decisions where the weights of the individual experts represent the reliability of the sources. The reliability measure used in the statistical approach is common to all pixels in previous work. In this experiment, the weight factors have been assigned to have different value for all pixels in order to improve the integrated classification accuracies. Although most implementations of Bayesian classification approaches assume fixed a priori probabilities, we have used adaptive a priori probabilities by iteratively calculating the local a priori probabilities so as to maximize the posteriori probabilities. The effectiveness of the proposed method is at first demonstrated on simulations with artificial and evaluated in terms of real-world data sets. As a result, we have shown that Bayesian statistical fusion scheme performs well on multispectral data classification.

• 한국융합학회논문지
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• 제9권12호
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• pp.99-106
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• 2018

의료방사선 방어를 위해 사용되는 차폐 시트의 제조과정에서 인장강도를 유지하면서 차폐 재료의 충전율을 높여 차폐 성능을 향상시키는 방안을 제시하고자 한다. 본 연구에서 제안된 개질제는 고분자 수지재료와의 융합에 있어서 분자량을 높여 재료의 친화성을 높이는 역할을 수행한다. 차폐시트의 산화텅스텐의 충전율과 인장강도, 차폐 성능 등을 평가하였다. 공정과정에서 개질제는 분자량과 밀도를 증가시켰고, 성형 과정에서 퍼짐 형상이 적용되었고, 성능 향상과 재료와의 친화성, 인장강도를 유지하기 위해 사용된 개질제 PMMA는 20%를 혼합할 경우 가장 우수한 효과를 얻을 수 있다. 본 연구에서 제시된 재료의 융합과 개질제를 통해 차폐시트의 대량생산이 가능하며, 향후 경량의 차폐복 제작에 기여할 것입니다.

• Korean Chemical Engineering Research
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• 제46권2호
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• pp.205-210
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• 2008

PLGA는 생분해성 고분자이며 생체적합성을 갖는 고분자로서 약물전달을 위한 연구에 많이 이용되고 있는 고분자이다. 그러나 PLGA를 약물전달에 이용할 때 약물의 급격한 초기방출 이후에 일정기간동안 약물이 방출되지 않는 지연시간이 존재하게 된다. 이러한 문제를 해결하기 위하여 PLGA 웨이퍼에 단량체를 첨가하여 제조하였다. 또한 PLGA를 재결정하여 약물의 급격한 방출 현상을 억제하도록 하였다. SEM을 이용하여 재결정된 PLGA의 형태학적 차이를 관찰하였으며 생체외 약물 방출거동은 HPLC를 이용하여 측정하였다. PLGA의 분해과정에서 단량체의 영향을 알아보기 위하여 GPC를 이용하여 분자량 변화를 측정하였다. 본 연구를 통하여 PLGA의 벌크분해에 기인한 지연시간을 없앨 수 있었으며 약물의 방출 기간을 3일까지 앞당길 수 있었다.

• 한국산업융합학회 논문집
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• 제5권1호
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• pp.27-32
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• 2002

In this study, estimate of Mechanical Properties depending on Injected direction in Polymer for Automobile Application. The used materials is polyamid(nylon6), polypropylene(PP) of a simple component and its mixed, reinforced glass fiber(15%) in polyamid. One side direction of a simple component is highest strength and the two sides direction of multicomponent appear lowest strength. This is caused by effects of weldline, imperfect fusion and impregnation of multicomponent. To improve mechanical properties, it is necessary to minimize of weldline, compatibility, perfect fusion and impregnation in multicomponent polymer.