• Title/Summary/Keyword: Fusion Weight

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Preparation and Characterization of PLGA Scaffold Impregnated Keratin for Tissue Engineering Application (케라틴이 함유된 조직공학적 PLGA 지지체의 제조 및 특성 분석)

  • Oh, A-Young;Kim, Soon-Hee;Lee, Sang-Jin;Yoo, James J.;Dyke, Mark van;Rhee, John M.;Khang, Gil-Son
    • Polymer(Korea)
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    • v.32 no.5
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    • pp.403-408
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    • 2008
  • Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin is useful as natural protein. We developed the keratin loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds (keratin/PLGA) for the possibility of the application of the tissue engineering using bone marrow mesenchymal (BMSCs). Keratin/PLGA (contents 0%, 10%, 20% and 50% of PLGA weight) scaffolds were prepared by solvent casting/salt leaching method. We characterized porosity, wettability, and water uptake ability, DSC of keratin/PLGA scaffold. We seeded BMSCs isolated from the femurs of rat into the inner core of the hybrid scaffold. Celluar viability were assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) test. We confirmed that keratin/PLGA scaffold is hydrophilic by wettability, and water uptake ability measurement results. In MTT assay results, cell viability in scaffolds impregnated 10 and 20 wt% of keratin were higher than other scaffolds. In conclusion, we suggest that keratin/PLGA scaffold may be useful to tissue engineering using BMSCs.

The Effects of Blend Composition and Blending Time on the Ester Interchange Reaction and Tensile Properties of PLA/LPCL/HPCL Blends

  • Yoon, Cheol-Soo;Ji, Dong-Sun
    • Fibers and Polymers
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    • v.4 no.2
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    • pp.59-65
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    • 2003
  • PLA/LPCL/HPCL blends composed of poly(lactic acid) (PLA), low molecular weight poly($\varepsilon$-caprolactone) (LPCL), and high molecular weight poly($\varepsilon$-caprolactone) (HPCL) were prepared by melt blending for bioabsorbable fila-ment sutures. The effects of blend composition and blending time on the ester interchange reaction by alcoholysis in the PLA/LPCL/HPCL blends were studied. Their thermal properties and the miscibility due to the ester interchange reaction were investigated by $^1{H-NMR}$, DSC, X-ray, and UTM analyses. The hydroxyl group contents of LPCL in the blends decreafed by the ester interchange reaction due to alcoholysis. Thus, the copolymer was formed by the ester interchange reaction at $200^{\circ}C$ for 30-60 minutes. The thermal properties of PLA/LPCL/HPCL blends such as melting temperature and heat of fusion decreased with increasing ester interchange reaction levels. However, the miscibility among the three poly-mers was improved greatly by ester interchange reaction. Tensile strength and modulus of PLA/LPCL/HPCL blend fibers increased with increasing HPCL content, while the elongation at break of the blend fibers increased with increasing LPCL content.

Visible Image Enhancement Method Considering Thermal Information from Infrared Image (원적외선 영상의 열 정보를 고려한 가시광 영상 개선 방법)

  • Kim, Seonkeol;Kang, Hang-Bong
    • Journal of Broadcast Engineering
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    • v.18 no.4
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    • pp.550-558
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    • 2013
  • The infrared and visible images are represented by different information due to the different wavelength of the light. The infrared image has thermal information and the visible image has texture information. Desirable results are obtained by fusing infrared and visible information. To enhance a visible image, we extract a weight map from a visible image using saturation, brightness. After that, the weight map is adjusted using thermal information in the infrared image. Finally, an enhanced image is resulted from combining an infrared image and a visible image. Our experiment results show that our proposed algorithm is working well to enhance the smoke in the original image.

Antifungal Effect of Chitosan as Ca2+ Channel Blocker

  • Lee, Choon Geun;Koo, Ja Choon;Park, Jae Kweon
    • The Plant Pathology Journal
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    • v.32 no.3
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    • pp.242-250
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    • 2016
  • The aim of this study was to investigate antifungal activity of a range of different molecular weight (MW) chitosan against Penicillium italicum. Our results demonstrate that the antifungal activity was dependent both the MW and concentration of the chitosan. Among a series of chitosan derived from the hydrolysis of high MW chitosan, the fractions containing various sizes of chitosan ranging from 3 to 15 glucosamine units named as chitooligomers-F2 (CO-F2) was found to show the highest antifungal activity against P. italicum. Furthermore, the effect of CO-F2 toward this fungus was significantly reduced in the presence of $Ca^{2+}$, whereas its effect was recovered by ethylenediaminetetraacetic acid, suggesting that the CO-F2 acts via disruption of $Ca^{2+}$ gradient required for survival of the fungus. Our results suggest that CO-F2 may serve as potential compounds to develop alternatives to synthetic fungicides for the control of the postharvest diseases.

Preparation and Crystallization Behavior of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Grafted with Poly(N-vinylpyrrolidone) (Poly(N-vinylpyrrolidone)이 그래프트된 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) 공중합체의 합성 및 결정화 거동)

  • Wang, Wei;Zhang, Yu;Chen, Yanmo
    • Polymer(Korea)
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    • v.31 no.5
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    • pp.385-392
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    • 2007
  • Poly (N-vinylpyrrolidone) (PVP) groups were grafted onto a poly(3-hydroxybutyrate-co-3-hydroryvalerate) (PHBV) backbone in order to modify its properties and synthesize a novel biocompatible copolymer. The crystallization behavior of PHBV and grafted PHBV was investigated by differential scanning calorimetry (DSC) and polarized optical microscopy (POM). During the cooling-induced crystallization process, the crystallization temperature and the crystallization rate of the grafted PHBV decreased with increasing PVP weight fraction. On the heating scans of all grafted PHBV samples, a new crystallization exothermic peak appeared at almost the same temperature, suggesting the operation of a recrystallization process, while the melting temperature ($T_m$) and the apparent enthalpy of fusion (${\Delta}H_f$) were not affected by graft modification. During the isothermal crystallization process at the same temperature, the presence of side PVP groups decreased the spherulitic growth rate and the spherulitic band spacing with increasing PVP weight fraction in samples.

Properties of Stretchable Electrode Pattern Printed on Urethane Film (우레탄 필름에 인쇄된 신축 가능한 전극 패턴의 특성)

  • Nam, Su-Yong;Kwon, Bo-Seok;Nam, Hyun-Jin;Nam, Ki-Woo;Park, Hyo-Zun
    • Journal of Power System Engineering
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    • v.22 no.1
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    • pp.64-71
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    • 2018
  • Currently, functional patterns are formed by screen printing on stretchable films, and they are applied to wearable and stretchable devices. In this study, three types of silver paste were prepared using three polyester binders with different Tg and molecular weights in order to impart elasticity to the conductive pattern itself. Rheological properties and DSC measurements were performed for each silver paste. Then, each silver paste was screen printing and cured by an IR dryer to evaluate adhesive strength, pencil hardness, resistance and surface shape change according to strain. As a result, it was found that the silver paste using a binder with a low Tg and a high molecular weight has the smallest resistance change depending on the strain. Namely, it was found that it is most preferable to use a binder with a low Tg and a high molecular weight as the stretchable electrode.

Reduction of GPS Latency Using RTK GPS/GNSS Correction and Map Matching in a Car NavigationSystem

  • Kim, Hyo Joong;Lee, Won Hee;Yu, Ki Yun
    • Journal of Korean Society for Geospatial Information Science
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    • v.24 no.2
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    • pp.37-46
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    • 2016
  • The difference between definition time of GPS (Global Positioning System) position data and actual display time of car positions on a map could reduce the accuracy of car positions displayed in PND (Portable Navigation Device)-type CNS (Car Navigation System). Due to the time difference, the position of the car displayed on the map is not its current position, so an improved method to fix these problems is required. It is expected that a method that uses predicted future positionsto compensate for the delay caused by processing and display of the received GPS signals could mitigate these problems. Therefore, in this study an analysis was conducted to correct late processing problems of map positions by mapmatching using a Kalman filter with only GPS position data and a RRF (Road Reduction Filter) technique in a light-weight CNS. The effects on routing services are examined by analyzing differences that are decomposed into along and across the road elements relative to the direction of advancing car. The results indicate that it is possible to improve the positional accuracy in the along-the-road direction of a light-weight CNS device that uses only GPS position data, by applying a Kalman filter and RRF.

Estimation of fresh weight for chinese cabbage using the Kinect sensor (키넥트를 이용한 배추 생체중 추정)

  • Lee, Sukin;Kim, Kwang Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.205-213
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    • 2018
  • Development and validation of crop models often require measurements of biomass for the crop of interest. Considerable efforts would be needed to obtain a reasonable amount of biomass data because the destructive sampling of a given crop is usually used. The Kinect sensor, which has a combination of image and depth sensors, can be used for estimating crop biomass without using destructive sampling approach. This approach could provide more data sets for model development and validation. The objective of this study was to examine the applicability of the Kinect sensor for estimation of chinese cabbage fresh weight. The fresh weight of five chinese cabbage was measured and compared with estimates using the Kinect sensor. The estimates were obtained by scanning individual chinese cabbage to create point cloud, removing noise, and building a three dimensional model with a set of free software. It was found that the 3D model created using the Kinect sensor explained about 98.7% of variation in fresh weight of chinese cabbage. Furthermore, the correlation coefficient between estimates and measurements were highly significant, which suggested that the Kinect sensor would be applicable to estimation of fresh weight for chinese cabbage. Our results demonstrated that a depth sensor allows for a non-destructive sampling approach, which enables to collect observation data for crop fresh weight over time. This would help development and validation of a crop model using a large number of reliable data sets, which merits further studies on application of various depth sensors to crop dry weight measurements.

Analysis of Factors Affecting the Periplasmic Production of Recombinant Proteins in Escherichia coli

  • Mergulhao, Filipe J.;Monteiro, Gabriel A.
    • Journal of Microbiology and Biotechnology
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    • v.17 no.8
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    • pp.1236-1241
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    • 2007
  • Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of the bacterial culture were analyzed regarding the expression and periplasmic secretion of the recombinant proteins. It was found that secretion was not affected by the size of the translocated peptide (17-42 kDa) and that the highest periplasmic production values were obtained on the exponential phase of growth. Moreover, the highest periplasmic values were obtained in minimal medium, showing the relevance of the culture medium composition on secretion. In silico prediction analysis suggested that with respect to the five proteins used in this study, those that are prone to form ${\alpha}$-helix structures are more translocated to the periplasm.

Effect of Coating Weight on the Laser Weldability in the Welding of Aluminized Steels (레이저용접에서 알루미늄 도금량이 용접성에 미치는 영향)

  • Kim Ki Chol;Cha Joon Ho
    • Korean Journal of Materials Research
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    • v.14 no.1
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    • pp.1-8
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    • 2004
  • Laser weldability of aluminized steels for deep drawing application has been investigated. Test coupons for Nd:YAG laser welding and $CO_2$ laser welding were prepared trom the commercial steels. According to the test results, total penetration and back bead width of aluminized steels were sensitive to the welding conditions. Bead width at the half thickness of the overlap joint, however, was rather constant. Laser weldability of aluminized steels was superior to that of zinc coated steel. Weld microstructure revealed that overlap zone adjacent to the fusion line was filled with coated materials, which was thought to be desirable to protect weld from crevice corrosion. The aluminum coated materials was also found in the weld metal. Practically no spattering was observed in the laser welding of aluminized steels even when the welding was performed without joint gap. In the welding of zinc coated steel, however, spattering was so severe that it was difficult to get the acceptable weld. Bead quality of aluminized steel laser weld was smooth and stable.