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Preparation and Characterization of PLGA Scaffold Impregnated Keratin for Tissue Engineering Application  

Oh, A-Young (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Soon-Hee (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Lee, Sang-Jin (Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard)
Yoo, James J. (Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard)
Dyke, Mark van (Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard)
Rhee, John M. (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Khang, Gil-Son (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Publication Information
Polymer(Korea) / v.32, no.5, 2008 , pp. 403-408 More about this Journal
Abstract
Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. Keratin is useful as natural protein. We developed the keratin loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds (keratin/PLGA) for the possibility of the application of the tissue engineering using bone marrow mesenchymal (BMSCs). Keratin/PLGA (contents 0%, 10%, 20% and 50% of PLGA weight) scaffolds were prepared by solvent casting/salt leaching method. We characterized porosity, wettability, and water uptake ability, DSC of keratin/PLGA scaffold. We seeded BMSCs isolated from the femurs of rat into the inner core of the hybrid scaffold. Celluar viability were assayed by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) test. We confirmed that keratin/PLGA scaffold is hydrophilic by wettability, and water uptake ability measurement results. In MTT assay results, cell viability in scaffolds impregnated 10 and 20 wt% of keratin were higher than other scaffolds. In conclusion, we suggest that keratin/PLGA scaffold may be useful to tissue engineering using BMSCs.
Keywords
keratin; scaffold; bone marrow mesenchymal stem cells (BMSCs);
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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