• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.4
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• pp.254-259
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• 2012

Peeling damage of trees is usually caused by Cervidae such as deer, roe deer because of the lack of food in forests. However, it happens as part of the developing of antlers in Jeju Island when the roe deer try to remove the Velvet-the skin of the antlers. The research area is the Hannam experimental forest (400 m up to 500 m above sea level) of Korea Forest Research Institute in Jeju Island, and the survey was carried out along the 6 km long of forest road with 5 m width on both sides. Twenty five tree species (total 267 stands) are damaged by peeling; 18 (134 stands) deciduous broad-leaved species, 5 (71 stands) in evergreen broad-leaved species, 2 (62 stands) coniferous species. The most common damaged species are in order of Daphniphyllum macropodum, Cryptomeria japonica, Lindera erythrocarpa, Clerodendrum trichotomum, Zanthoxylum schinifolium. Mainly damaged trees are approximately 3~4 years old saplings, and they show the mean height $120.7{\pm}42.4cm$, diameter measured at 5 cm height $1.5{\pm}0.5cm$. The Lowest peeling beginning height is $22.1{\pm}10.1cm$, and the mean length of peeling is $27.5{\pm}10.6cm$. Once the peeling damage happens, the saplings are infected by fungi secondly, and are distorted or dead, therefore the future structure of warm-temperate forests could be in influenced in species. Warm-temperate forest landscape and species change related to the climate change is a rising issue in Jeju Island. However the changes caused by peeling damage also could be an important issue in the natural process of forest environment, afforestation, local nursery and sustainable forest management of Jeju Island.

• The Korean Journal of Pesticide Science
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• v.9 no.1
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• pp.26-34
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• 2005

3D-QSAR studies for the fungicidal activities against resistance phytophthora blight (RPC; 95CC7303) and sensitive phytophthora blight (Phytopthora capsici) (SPC; 95CC7105) by a series of new 2-alkoxyphenyl-3-phenylthioisoindoline-1-one derivatives (A & B) were studieded using comparative molecular similarity indices analyses (CoMSIA) methodology. From the based on the results, the two CoMSIA models, R5 and S1: as the best models were derivated. The statistical results of the models showed the best predictability and fitness for the fungicidal activities based on the cross- validated value ($q^2=0.714{\sim}0.823$) and non cross-validated, value ($r^2_{ncv.}=0.918{\sim}0.954$), respectively. The model R5 for fungicidal activity of RPC generated from the field fit alignment and combination of electrostatic field, H-bond acceptor field and LUMO molecular orbital field. The model S1 (or S5) for fungicidal activity of SPC generated from the atom based fit alignment and combination of steric field and HOMO molecular orbital field. The models also shows that inclusion of H-bond acceptor field (A) improved the statistical significance of the models. From the based graphical analyses of CoMSIA contribution maps, it was revealed that the novel selective character for fungicidal activities between the two fungi by modify of X-sub-stituent on the N-phenyl group and R-substituent on the S-phenyl group will be able to achivement.

• Journal of Korean Society of Forest Science
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• v.79 no.3
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• pp.302-308
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• 1990

This study was conducted to determine the effects of mycorrhizal inoculation and ortet ages on the rooting of Quercus acutissima cuttings. The cuttings taken from 12-week-, 2-year-, 4-year-, and 20-year-old plants were rooted in mid summer in the rooting medium (vermiculite 2 : peatmoss 1 by volume) with or without Pisolithus tinctorius (Pt) and Glomus sp. inocula and IBA under intermittent misting system in an open shed. The average percentages of rooting were about 82%, 49%, 29%, and 13% for cuttings taken from 12-week-, 2-year-. 4-year-, and 20-year-old seedlings, respectively. Pt inoculation, enhanced rooting of cuttings at all age classes, except 12-week-old seedlings, with the highest enhancement(22%) observed in cuttings taken from 20-year-old trees. The highest percentage of rooting in each age group eras 88.9% in 12-week-old seedlings treated with Pt plus 3.000ppm IBA, 75% in 2-Year-old plants with 1,000ppm IBA, 58.3% in 4-year-old plants with 3.000ppm IBA and 22% in 20-year-old plants. The addition of Glomus sp. fungus inoculum failed to enhance rooting. Pt mycorrhizal inoculation enhanced root dry weight, length, and diamter of adventitious roots at cuttings taken from 12-week- and 20-year-old trees, except the cuttings taken from 4-year-old seedlings. Rooted cuttings had more total nitrogen content in the leaves than unrooted cuttings, and the greater rooting response was associated with the higher phosphorus content in the leaves.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.329-339
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• 1996

This study is to investigate the effect of mycorrhizal development, growth, nutrient absorption of P. thunbergii seedlings inoculated with Pisolithus tinctorius(Pt. KJ-1) in relation to toxic materials in soil. The concentrations on copper solution applied to the soils were 0, 40, 120, 260, 430ppm. The results are summarized as follows : 1. The germination of P. thunbergii with Pt. increased greater than without ectomycorrhizal fungi. Mycorrhizal development showed a significant decreasing trend at high concentration, and tolerant Cu test with Pt. in agar plate media showed a decreasing trend at a high level. 2. P. thunbergii seedlings inoculated with Pt. showed that the shoot length was significantly promoted at 40, 120ppm copper levels, and that of noninoculated seedlings had the lowest effect in 430ppm copper level. 3. The outer shape of ectomycorrhizal root tips after inoculating Pt. on P. thunbergii seedlings appeared as a monopodial type, a fern-like type, and a cluster-like type at 0ppm, 40ppm levels, but only monopodial type came out at 260ppm, 430ppm copper levels. 4. Root length, no. of juvenile leaves, total length of juvenile leaves, total dry weight, no. of needles and total length of needles of P. thunbergii with Pt. increased greater than those of noninoculated seedling. Growth response of P. thunbergii seedling inoculated with Pt. increased significantly at 40ppm, 120ppm Cu levels. 5. As a result of analysis of growth medium, pH, Na, CEC increased at higher Cu level, and total nitrogen, organic matters, available phosphorous, K, Ca and Mg decreased at 40ppm, 120ppm Cu levels. As a result of an analysis about a copper within soils, it appeared having high Cu-concentration at 0ppm level of an inoculated Pt. and high Cu-concentration in noninoculated Pt. than in inoculated Pt. at higher level. 6. As a result of an analysis of shoot, N, P, and K-concentration were higher in noninoculated seedlings than in inoculated seedlings, and Cu-concentration was higher in inoculated seedlings than in noninoculated seedlings. The analysis of root resulted in a high N-concentration at 40ppm, 120ppm levels, in a high P-concentration in inoculated seedlings and in a high Cu-concentration in noninoculated seedlings. Cu-concentration was significantly higher at root than at shoot.

• Journal of Periodontal and Implant Science
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• v.30 no.4
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• pp.851-870
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• 2000

The major goals of periodontal therapy is the functional regeneration of periodontal supporting structures already destructed by periodontal disease as well as the reduction of signs and symptoms of progressive periodontal disease. There have been many efforts to develop materials and therapeutic methods to promote periodontal wound healing. There have been increasing interest on the chitosan made by chitin. Chitin is second only to cellulose as the most abundant natural biopolymer. It is a structural component of the exoskeleton of invertebrates(e.g., shrimp, crabs, lobsters), of the cell wall of fungi, and of the cuticle of insects. Chitosan is a derivative of chitin made by deacetylation of side chains. Many experiments using chitosan in various animal models have proven its beneficial effects. The aim of this study is to evaluate the osteogenesis of chitosan on the calvarial critical size defect in Sprague Dawley rats. An 8 mm surgical defect was produced with a trephine bur in the area of the midsagittal suture. The rats were divided into two groups: Untreated control group versus experimental group with 50mg of soluble chitosan gel. The animals were sacrificed at 2, 4 and 8 weeks after surgical procedure. The specimens were examined by histologic, histomorphometric and radiodensitometric analyses. The results are as follows: 1. The length of newly formed bone in the defects was $102.91{\pm}25.46{\mu}m$, $219.46{\pm}97.81{\mu}m$ at the 2 weeks, $130.95{\pm}39.24{\mu}m$, $212.39{\pm}89.22{\mu}m$ at the 4 weeks, $181.53{\pm}76.35{\mu}m$ and $257.12{\pm}51.22{\mu}m$ at the 8 weeks in the control group and experimental group respectively. At all periods, the means of experimental group was greater than those of control group. But, there was no statistically significant difference between the two groups. 2. The area of newly formed bone in the defects was $2962.06{\pm}1284.48{\mu}m^2$, $5194.88{\pm}1247.88{\mu}m^2$ at the 2 weeks, $5103.25{\pm}1375.88{\mu}m^2$, $7751.43{\pm}2228.20{\mu}m^2$ at the 4 weeks and $8046.20{\pm}818.99{\mu}m^2$, $15578.57{\pm}5606.55{\mu}m^2$ at the 8 weeks in the control group and experimental group respectively. At all periods, the means of experimental group was greater than those of control group. The experimental group showed statistically significant difference to the control group at the 2 and 8 weeks. 3. The density of newly formed bone in the defects was $14.26{\pm}6.33%$, $27.91{\pm}6.65%$ at the 2 weeks, $20.06{\pm}9.07%$, $27.86{\pm}8.20%$ at the 4 weeks and $22.99{\pm}3.76%$, $32.17{\pm}6.38%$ at the 8 weeks in the control group and experimental group respectively. At all periods, the means of experimental group was greater than those of control group. The experimental group showed statistically significant difference to the control group at the 2 and 8 weeks. These results suggest that the use of chitosan on the calvarial defects in rats has significant effect on the regeneration of bone tissue in itself

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.743-750
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• 2006

Poplars and willow were planted to identify suitable species and varieties for landfill reclamation at the Sudokwon Landfill Site in 1997. Survival rate, growth performance, vitality, visible foliar injury by pollutants, fungi, and leaf insects, and stem borer damage have been investigated for 10 clones of 4 poplar species and 2 clones of one willow species from 1997 to 2005. The average survival rates of poplar and willow clones were drastically decreased from 90% in 1997 to 53% in 2005. Among poplar species, Populus alba ${\times}$ P. glandulosa showed the highest mean survival rate of 66%, while Populus koreana ${\times}$ P. nigra var. italica and Populus euramericana were the lowest of 41%, respectively in 2005. Clivus, which is one of the clones from Populus alba ${\times}$ P. glandulosa, showed the highest survival rate of 73%. For mean height, Ec028 clone(P. euramericana) showed the highest of $11.2m{\pm}2.1m$ and followed by Clivus of $11.0m{\pm}2.0m$. Clone 131-27(Salix alba) was the lowest of $7.8m{\pm}1.6m$. Vitality, defoliation, visible foliar damage, and stem borer damage were significantly different among clones. Most of Populus alba ${\times}$ P. glandulosa and Salix alba clones seemed to have strong vitality and to be tolerant to various stresses at the site. However, Populus nigra ${\times}$ P. maximowiczii was sensitive to the stress. We have selected 5 clones in total: Clivus as the best clone for waste landfill reclamation, and additionally two Salix clones 131-25, 131-27 and two clones of Populus alba ${\times}$ P. glandulosa (72-9, 72-16) have been selected. These five clones could be supplied for planting at sites having an environment similar to the Sudokwon Landfill Site.

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.735-742
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• 2006

The objective of this study was to elucidate the tolerance of woody plants to simulated acid rain in relation to mycorrhizal inoculation. Germinating seedlings of Robinia pseudoacacia were planted in 1I pots with autoclaved soil mixture of vermiculite, sand and nursery soil at 1:1:1 ratio. Each pot was inoculated with both crushed root nodules from a wild tree of the same species and commercial arbuscular mycorrhizal inoculum of Glomus intraradices at the time of planting the seedlings. Simulated acid rains at pH 2.6, 3.6, 4.6, and 5.6 were made by mixing sulfuric acid and nitric acid at 3: 1 ratio. Each pot received nutrient solution without N and P, and was also supplied with 180 ml of the one pH level of the acid rains once a week for 50 days. The plants were grown in the green house. At the end of experimental period, plants were harvested to determine contents of chlorophyll, mineral nutrients and net photosynthesis in the tissues, dry weight of the plants, and mycorrhizal infection in the roots. Mycorrhizal infection rate was significantly reduced only at pH 2.6, which meant vitality of G intraradices was inhibited at extremely low pH. Height growth, dry weight production, nodule production and chlorophyll content were increased by mycorrhizal infection in all the pH levels except pH 3.6. Particularly, mycorrhizal inoculation increased root nodule production by 85% in pH 5.6 and 45% in 4.6 treatments. But the stimulatory effect of mycorrhizal inoculation on nodule production was reduced at pH 3.6 and 2.6. Net photosynthesis was increased by mycorrhizal infection in all the pH levels. The phosphorus(P) content in the tissues was increased by 43% in average by mycorrhizal inoculation, which was statistically significant except in pH 2.6. It was concluded that mycorrhizal inoculation of Robinia pseudoacacia would enhance growth and resistance of the plants to acid rain by improving the photosynthesis, phosphorus nutrition, and more nodule production.

• Journal of the East Asian Society of Dietary Life
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• v.18 no.5
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• pp.732-738
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• 2008

This study was carried out to examine information regarding nutritional intake according to intake of health improving agents. For this study, 593 adults were interviewed face-to-face. The average ages of the participants were 58.8 years old in males and 56.1 years old in females. The percentage of individuals taking agents was significantly higher (p<0.05) among the females (22.1%) than the males (14.1%). Men showed a preference for taking animal protein bases restorative food and health supplements, while women preferred health supplements and nutritional supplements as improving agents. However, there were no significant differences between the two groups. The total daily food intake was also not significantly different according to intake of improving agents in the male and female groups. The male subjects who took improving agents showed significantly lower intake of potato and starches, pulses, fungi and mushrooms, seaweeds, oils and fat, as well as seasoning than those of non-users. Female subjects who took these agents also had a significantly lower intake of pulses, nuts and seeds than nonusers. No difference in the daily energy intake was reported in male and female groups whether the individuals were taking improving agents in male and female groups or not. However, in male subjects who took improving agents, there was a significantly lower intake of protein, dietary fiber, carotene, vitamin $B_1$, folate, vitamin C, vitamin E, plant calcium, potassium and plant iron than the male non-users. This study suggests that practical education be emphasized for proper nutrition intake thorough nutritional management according to improving agents.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.417-422
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• 2010

Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at $20{\sim}25^{\circ}C$ and suppressed above $28^{\circ}C$. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of $10^5$, $10^6$, $10^7$ and $10^8$ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and $LT_{50}$ values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity(98.6%) compared to single treatment of L. lecani Btab01 (79.9%).

• Journal of Life Science
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• v.22 no.9
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• pp.1187-1193
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• 2012

This study was conducted to evaluate effects of halogenated compounds on in vitro rumen fermentation characteristics and methane emissions. A fistulated Holstein cow of 650 kg body weight was used as a donor of rumen fluid. Five kinds of halogenated compounds (bromochloromethane (BCM), 2-bromoethane sulfonic acid (BES), 3-bromopropanesulfonic acid (BPS), chloroform (CLF), and pyromellitic diimide (PMDI) known to inhibit methyl-coenzyme M reductase activity were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, protozoa, and fungi were enumerated, and gas production including methane and fermentation characteristics were observed in vitro. The pH values ranged from 6.25 to 6.72 in all the treatments, and these showed a similar level at 48 hr. The total gas production in the treatments showed a similar pattern with C at 48 hr, whereas methane production in the treatments was lower (p<0.05) than C. Concentrations of total volatile fatty acids (VFAs) and propionic acid were higher (p<0.05) in the treatments than in C at 12 hr. Therefore, halogenated compounds (BCM, BES, BPS, CLF, and PMDI) inhibited in vitro methane emissions by inhibiting methanogens in the rumen. Further studies on safety are needed.