Ectomycorrhizal Development and Growth of Pinus thunbergii Seedlings Inoculated with Pisolithus tinctorius(KJ-1) in Copper Treated Soil

Cu를 처리(處理)한 토양(土壤)에서 Pisolithus tinctorius균(菌)을 접종(接種)한 곰솔유묘(幼苗)의 외생균근발달(外生菌根發達)과 생장(生長)

This study is to investigate the effect of mycorrhizal development, growth, nutrient absorption of P. thunbergii seedlings inoculated with Pisolithus tinctorius(Pt. KJ-1) in relation to toxic materials in soil. The concentrations on copper solution applied to the soils were 0, 40, 120, 260, 430ppm. The results are summarized as follows : 1. The germination of P. thunbergii with Pt. increased greater than without ectomycorrhizal fungi. Mycorrhizal development showed a significant decreasing trend at high concentration, and tolerant Cu test with Pt. in agar plate media showed a decreasing trend at a high level. 2. P. thunbergii seedlings inoculated with Pt. showed that the shoot length was significantly promoted at 40, 120ppm copper levels, and that of noninoculated seedlings had the lowest effect in 430ppm copper level. 3. The outer shape of ectomycorrhizal root tips after inoculating Pt. on P. thunbergii seedlings appeared as a monopodial type, a fern-like type, and a cluster-like type at 0ppm, 40ppm levels, but only monopodial type came out at 260ppm, 430ppm copper levels. 4. Root length, no. of juvenile leaves, total length of juvenile leaves, total dry weight, no. of needles and total length of needles of P. thunbergii with Pt. increased greater than those of noninoculated seedling. Growth response of P. thunbergii seedling inoculated with Pt. increased significantly at 40ppm, 120ppm Cu levels. 5. As a result of analysis of growth medium, pH, Na, CEC increased at higher Cu level, and total nitrogen, organic matters, available phosphorous, K, Ca and Mg decreased at 40ppm, 120ppm Cu levels. As a result of an analysis about a copper within soils, it appeared having high Cu-concentration at 0ppm level of an inoculated Pt. and high Cu-concentration in noninoculated Pt. than in inoculated Pt. at higher level. 6. As a result of an analysis of shoot, N, P, and K-concentration were higher in noninoculated seedlings than in inoculated seedlings, and Cu-concentration was higher in inoculated seedlings than in noninoculated seedlings. The analysis of root resulted in a high N-concentration at 40ppm, 120ppm levels, in a high P-concentration in inoculated seedlings and in a high Cu-concentration in noninoculated seedlings. Cu-concentration was significantly higher at root than at shoot.

곰솔(Pinus thunbergii)유묘(幼苗)에 Pisolithus tinctorius(Pt., KJ-1)를 접종(接種)하고 $CuCl_2$용액을 0, 40, 120, 260, 430ppm 농도(濃度)로 처리수준(處理水準)을 달리하여 균근형성(菌根形成)및 발달(發達), 유묘생장(幼苗生長), 양분흡수(養分吸收)에 미치는 영향(影響)을 생장(生長) 시기별(時期別)(6월(月) 1일(日), 7월(月) 1일(日), 8월(月) 1일(日), 9월(月) 1일(日), 10월(月) 1일(日), 11월(月) 1일(日))로 조사(調査)하여 얻어진 결과(結果)는 다음과 같다. 1. 곰솔종자(種子)의 발아율(發芽率)은 균근접종구(菌根接種區)가 비접종구(非接種區)보다 높았다. 곰솔유묘(幼苗)의 균근형성율(菌根形成率)은 고농도(高濃度)로 갈수록 현저히 감소(減少)하였으며, Pt. 균(菌)의 Cu 내성조사(耐性調査)를 위해 실시한 MMN Agar평판배지실험(培地實驗)에서도 고농도(高濃度)로 갈수록 생장(生長)이 감소(減少)하였다. 2. 곰솔유묘(幼苗)의 묘고생장(苗高生長)에서 접종구(接種區) 40ppm구(區)와 120ppm구(區)에서 생장효과(生長效果)가 크게 나타났으며, 비접종구(非接種區) 430ppm구(區)에서 가장 저조(低調)했다. 3. 균근(菌根)의 외부형태(外部形態)는 0, 40ppm구(區)에서는 monopodial type, fern-like type, cluster-like type이 나타났으나, 260, 430ppm에서는 monopodial type만이 나타났다. 4. 접종구(接種區)에서 근(根)길이, 유기엽(幼期葉)의 수(數), 유기엽(幼期葉)의 총(總)길이, 총건중량(總乾重量), 본엽수(本葉數), 본엽총(本葉總)길이 등(等)이 비접종구(非接種區)보다 높게 나타났으며, 일반적(一般的)으로 접종구(接種區) 40, 120ppm구(區)에서 생장효과(生長效果)가 크게 나타났다. 5. 곰솔유묘(幼苗) 생육배지(生育培地)인 토양분석(土壤分析) 결과(結果)는 pH, Na, CEC는 고농도(高濃度)로 갈수록 증가(增加)하는 경향(傾向)을 나타내었고, 전질소(全窒素), 유기물(有機物), P, K, Ca, Mg는 40, 120ppm구(區)에서 감소(減少)하였다. 토양내(土壤內) Cu 분석결과(分析結果) 0ppm구(區)에서는 접종구(接種區)에서 높게 나타났으며, 고농도(高濃度)로 갈수록 뚜렷하게 비접종구(非接種區)에서 높은 함량(含量)이 나타났다. 6. 지상부(地上部) 분석결과(分析結果) 접종구(接種區)에서 전질소(全窒素), P, K의 흡수(吸收)가 많았고, 비접종구(非接種區)에서 Cu 흡수(吸收)가 많았다. 지하부(地下部) 분석결과(分析結果) 전질소(全窒素)는 생장(生長)이 좋은 40, 120ppm구(區)에서 많은 함량(含量)을 나타내었고, P은 비접종구(非接種區)보다 접종구(接種區)에서 아주 높은 흡수(吸收)가 있었고, Cu는 비접종구(非接種區)에서 높은 함량(含量)을 나타냈다. 그리고 모든 처리구(處理區)에서 지상부(地上部)보다 지하부(地下部)에서 현저(顯著)하게 높은 Cu 함량(含量)이 나타났다.