• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.457-462
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• 2020

Pancreatic cancer is among the most difficult-to-treat tumors. More than half of patients with this cancer have very few symptoms at the early stages, allowing the development of distant metastases and resistance to cancer treatment. In this study, we found that Juniperus chinensis extract (JCX) decreased the cell viability and migration activity of PANC-1 and SNU-213 pancreatic cancer cells in a dose-dependent manner. JCX increased caspase-3 activation and generation of reactive oxygen species (ROS). N-acetylcysteine treatment blocked JCX-induced ROS generation and the negative effects on pancreatic cancer cell viability. In addition, JCX down-regulated the levels of phospho-focal adhesion kinase (p-FAK) and phospho-extracellular signal-regulated kinase (p-ERK). Together, these results indicate that JCX induces apoptosis in human pancreatic cancer cell lines through ROS production, downregulating FAK/ERK signaling and activating caspase-3. We propose that JCX-derived compounds represent candidates for the development of alternative medicines for the treatment of pancreatic cancer.

• 한국환경생태학회지
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• 제29권1호
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• pp.14-20
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• 2015

We created species richness maps of mammals, birds and plants using "Nnational Ecosystem Survey" data and identified correlations between species richness maps of each taxa. We examine the distribution of species richness of each taxa and calculated conservation priority rank through plotting species-area curves using an additive benefit function in Zonation. The conclusions of this study are as follows. First, plant showed high species richness in Gangwon province and Baekdudaegan, and mammals showed high species richness at eastern slope of Baekdudaegan in Gangwon province unusually and the species richness of mammals distributed equally except Gyeonggi and Chungnam province. However, birds showed high species richness in the west costal because the area is the major route of winter migratory birds. Second, correlation of each taxa's distribution is not significant. Correlation between mammals and birds is positive but correlations between birds and others are negative. Because mammals inhabit in forest but birds mostly live in coastal wetlands and rivers. Therefore, bird's habitats are not shared with other habitats. Third, the probability of mammals occurrence is very low under 25% in species-area curve, others increase proportionally to area. Birds increase dramatically richness at 10% because bird's habitat is concentrated in coastal wetlands and rivers. Plants increased gently species richness due to large forest in Gangwon province. We can calculate the predicted number of species in curves and plan various conservation strategies using the marginal number of species. Finally, high priority ranks for conservation distributed mainly in Gangwon province and Baekdudaegan. When we compared with priority map and terrestrial national parks, the parks were evaluated as high priority ranks. However, the rank of parks away from Baekdudaegan was low. This study has the meaning of selecting conservation priority area using National Ecosystem Survey. In spite of the omission of survey data in national parks and Baekdudaegan, the results were good. Therefore, the priority rank method using species distribution models is useful to selecting protected areas and improving conservation plans. However, it is needed to select protected areas considering various evaluation factors, such as rarity, connectivity, representativeness, focal species and so on because there is a limit to select protected area only using species richness.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권2호
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• pp.103-114
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• 2022

Zoological Parks house exclusive animal species, thus creating a source of education and awareness for visitors. Big cats like tigers and leopards are among the most visited species in zoos globally. However, they often display stressful or stereotypic behaviours. Such behaviours are influenced by multiple factors including visitors, animal history, and captive environment. To understand this impact, we investigated the behavioural response of tigers and leopards to visitation, captive, and biological factors. The behaviour of eight big cats housed in the National Zoological Park, New Delhi, was monitored using focal sampling technique during May and June 2019. We recorded the captive and biological factors and visitor density for the subjects. The study revealed high proportions of inactive and stereotypic behaviours amongst the species. Tigers and leopards were found to perform stereotypic behaviours for 22% and 28% of their time, respectively. Generalised Linear Models revealed a significant variation of stereotypy in association with the factors. Stereotypy was influenced by visitor density, age, sex, breeding history, coat colour, and enclosure design. Adults, males, white-coated, previously bred, and those housed in smaller and simple enclosures display more stereotypy than young, females, normal-coated, unbred, and those housed in larger and complex enclosures, respectively. A high density of visitors induced more stereotypic behaviours amongst the big cats. As providing entertainment and awareness amongst the public is one of the fundamental objectives of the zoo, visitors can not be avoided. Thus, we suggest providing appropriate enrichments that would reduce stereotypies and promote naturalistic behaviours.

• 생명과학회지
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• 제26권4호
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• pp.475-483
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• 2016

본 연구는 동의보감을 근거로 선별된 백수오, 지황, 원지 및 석창포로 구성된 복합추출물의 해마신경세포에 대한 보호 및 기억력의 개선효과를 살펴보았다. 복합추출물의 신경보호효과를 검증하기 위해 HT22해마신경세포의 생존율, 세포독성 및 활성산소를 분석하였으며, 허혈성 뇌손상 마우스모델을 이용하여 기억에 대한 동물행동학적 변화와 단백질 발현을 측정하였다. 해마신경세포에서 복합추출물의 전 처리는 glutamate에 의해 유도된 활성산소의 축적을 억제하였으며 세포사멸을 감소시켰다. 허혈성 뇌손상 마우스모델에서 복합추출물은 동물행동학적으로 공간 및 단기 기억능력을 개선시켰다. 뇌허혈로 인해 증가된 p38 MAPK의 인산화는 복합추출물에 의해 현저히 감소하는 반면, 감소된 PI3K와 CREB의 인산화는 현저히 증가하였다. 이를 면역조직화학분석을 통해 복합추출물을 투여한 그룹이 해마에서 발현되는 CREB의 인산화가 현저히 증가되는 것을 확인하였다. 이상의 결과는 복합추출물이 CREB 단백질과 관련된 신경보호 신호기전을 조절함으로써 인지기능을 개선시키는 것으로 사료된다.

• 대한수의학회지
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• 제48권4호
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• pp.469-472
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• 2008

Necropsy was performed on a total of twenty three either white leghorn or ogol chickens which were more than 150 weeks of age. Among twenty three chickens examined, fifteen chickens were laying and the rest eight chickens were non-laying. On necropsy, neoplastic mass in the five chickens among non-laying chickens was found. These neoplastic masses were present mostly in the ovaries and one case in the liver and characterized by multifocal to coalescing 1 to 5 mm tan firm nodular formation. On histopathology, ovarian adenocarcinoma with widespread abdominal seeding and hepatic metastasis was diagnosed in the three chickens. Oviductal leiomyoma was also found in two chickens that had a focal well-demarcated nodules in the oviduct. Taken together, the number with ovarian adenocarcinoma among non-laying chickens over 150 week old was 37.5%. As most animal species do rarely develop ovarian tumors, the high rate of spontaneous ovarian adenocarcinoma in non-laying hen suggest that the hen is a proper model for human ovarian cancer study.

• Clinical and Experimental Pediatrics
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• 제46권6호
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• pp.602-605
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• 2003

저자들은 salmonella group D로 인한 장염 및 패혈증이 동반된 음낭내 농양을 진단하여 항생제와 절개 및 배농으로 치료한 1례를 경험하였기에 보고하는 바이다.

• 한국미생물·생명공학회지
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• 제17권6호
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• pp.634-644
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• 1989

Listeria monocytogenes, one of five species in the genus Listeria and the only one currently believed to be pathogenic for humans, is a small gram-positive, nonsporeforming, aerobic, motile and hemolytic rod-shaped bacterium. The bacterium is widespread in the environment, having been isolated from soil, dust, animal feed, water, sewage, almost every type of animal that has been cultured, and asymptomatic humans. L. monocytogenes causes listeriosis, a disease which most often affects humans with a compromised immune system. Included are pregnant woman, infants and adults suffering from such diseases as cancer, cirrhosis of liver or AIDS or are being treated with drugs such as corticosteroids. Listeriosis is manifested by such syndromes as pregnancy infections, granulomatosis infantiseptica, sepsis, meningoencephalitis, and focal infections. Infections, can be treated successfully with penicillin, ampicillin, or erythromycin. However, a mortality rate of about 30％ has occurred in outbreaks of listeriosis. Food-associated outbreaks of listeriosis have been attributed to coleslaw (Canada, 1981), pasteurized milk (U.S., 1983), and soft cheese (U.S., 1985). Presence of L. monocytogenes in various dairy foods has prompted recall of such products from the U.S. market-place. L. monocytogenes also has been found in raw meats and seafood.

• Journal of Chest Surgery
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• 제34권7호
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• pp.574-577
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• 2001

동성애로 인하여 인간면역결핍 바이러스에 감염된 54세 남자가 발열, 우측흉통을 주소로 응급실로 내원하였다. 흉부단순촬영상 우측 흉막삼출액이 관찰되어 폐쇄식 흉관삽입술을 시행하였으며 흉막액 세균배양검사에서 살모넬라와 대장균이 동정되었고 항생제 투여에도 불구하고 증상 호전이 없어 늑막전폐절제술을 시행하였다. 수술 후에 환자상태는 호전되는 양상이었으나 수술 후 10일째 부분발작을 보여 뇌 단층촬영을 시행하였으며 우측 전두엽과 좌측 전측두엽에 여러 개의 종괴가 관찰되었고 수술 후 12일째 의식이 저하되면서 급성 호흡부전으로 수술 후 14일째 사망하였다.

• Archives of Plastic Surgery
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• 제36권6호
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• pp.685-690
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• 2009

Purpose: Autologous fat graft is a widely accepted technique used for soft tissue augmentation. Nonetheless, the use of fat graft is limited by unpredictable survival rates and repeated grafting. To avoid repeated grafting, cryopreserved fat graft technique has recently been widely used. On the other hand, the number of patients with chronic infection(who received cryopreserved fat injection) has currently been increasing. Therefore, this study was focused on the safety of cryopreserved fat injection from the infection. Methods: We collected 150 samples from local aesthetic clinics to examine the safety of cryopreserved autologous fat. To test for microbacterial contaminations of the cryopreserved fat specimens, microbacterial cultures & antibiotics sensitivity tests were performed. Then, we examined possible correlation between the preservation period and donor sites, focused on the results of microbacterial culture. Results: Cultures were positive for Staphylococcus epidermidis in 5 samples(methicillin - resistant Staphylococcus epidermidis in 4 samples), Micrococcus species in 3 samples. An average duration of preservation was 191 days and there was no significant correlation between the duration of preservation and microbacterial growth. Conclusion: Staphylococcus epidermidis was the leading cause of cryopreserved fat contamination, and the resistance to methicillin is common. Based on the above results, aseptic handling of fat during harvesting and preservation appeared to be most important.

• 농촌계획
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• 제24권2호
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• pp.21-29
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• 2018

Riparian management has become important as stream water quality as well as riparian ecosystem gain more public attentions. The objective of this study was to determine riparian widths based on the functions of nutrient removal and wildlife habitat protection and to apply for the Jinwee stream area as a preliminary case. Nitrogen and phosphorus filtration efficiencies were considered in water quality aspect, while the habitat radii of amphibian and reptiles were used for wildlife conservation purpose. In addition, observation of endangered species and human impact on wildlife disturbance in riparian area were also taken into account in determining riparian widths. The stream confluence zone was emphasized by doubling the riparian widths as the focal point for wildlife habitat conservation. As the results, three different levels of riparian widths were proposed depending on the major riparian functions and applied to the Jinwee stream section as the case study. The proposed method can be used to determine riparian width in other stream areas based on different functional focus, ie, water quality or riparian conservation purposes.