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http://dx.doi.org/10.5352/JLS.2016.26.4.475

New Four-herb Formula Ameliorates Memory Impairments via Neuroprotective Effects on Hippocampal Cells  

Ahn, Sung Min (Department of Korean Medical Science, School of Korean Medicine, Pusan National University)
Choi, Young Whan (Department of Horticultural Bioscience, College of Natural Resource and Life Science, Pusan National University)
Shin, Hwa Kyoung (Department of Korean Medical Science, School of Korean Medicine, Pusan National University)
Choi, Byung Tae (Department of Korean Medical Science, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.26, no.4, 2016 , pp. 475-483 More about this Journal
Abstract
The current study was conducted to evaluate beneficial effects of a new formula (CWC-9) using four traditional Oriental medicinal herbs, Cynanchum wilfordii, Rehmannia glutinosa, Polygala tenuifolia, and Acorus gramineus, on hippocampal cells and memory function. To examine the neuroprotective effects of a new four-herb extract, cell viability, cytotoxicity, and reactive oxygen species (ROS) assays were performed in HT22 cells and behavioral tests (Morris water maze and passive avoidance retention), Western blot, and immunohistochemistry were performed in a mouse model of focal cerebral ischemia. In HT22 hippocampal cells, pretreatment with CWC-9 resulted in significantly reduced glutamate-induced cell death with suppression of ROS accumulation caused by glutamate. In a mouse model of focal cerebral ischemia, we observed significant improvement of spatial and short-term memory function by treatment with CWC-9. Phosphorylated p38 mitogen-activated protein kinases (MAPK) in hippocampus of ischemic mice were decreased by treatment with CWC-9, but phosphorylated phosphatidylinositol-3 kinase (PI3K) and cAMP response element binding protein (CREB) were significantly enhanced. By immunohistochemical analysis, we confirmed higher expression of phosphorylation of CREB in the hippocampal neurons of CWC-9 treated mice. These results suggest that new multi-herb formula CWC-9 mainly exerted beneficial effects on cognitive function through regulation of neuro-protective signaling pathways associated with CREB.
Keywords
Focal cerebral ischemia; hippocampal neurons; memory; neuroprotection;
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