Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Juniperus chinensis extract induces apoptosis via reaction oxygen species (ROS) generation in human pancreatic cancer cell lines

  • Go, Boram (Department of Biotechnology, College of Applied Life Science, SARI, Jeju National University) ;
  • Han, Song-I (Subtropical/tropical organism gene bank, jeju National University) ;
  • Lee, Jungwhoi (Subtropical/tropical organism gene bank, jeju National University) ;
  • Kim, Da-Hye (Department of Biotechnology, College of Applied Life Science, SARI, Jeju National University) ;
  • Kim, Chang-Sook (Department of Biotechnology, College of Applied Life Science, SARI, Jeju National University) ;
  • Kim, Jae Hoon (Department of Biotechnology, College of Applied Life Science, SARI, Jeju National University)
  • Received : 2020.09.29
  • Accepted : 2020.12.06
  • Published : 2020.12.31
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Abstract

Pancreatic cancer is among the most difficult-to-treat tumors. More than half of patients with this cancer have very few symptoms at the early stages, allowing the development of distant metastases and resistance to cancer treatment. In this study, we found that Juniperus chinensis extract (JCX) decreased the cell viability and migration activity of PANC-1 and SNU-213 pancreatic cancer cells in a dose-dependent manner. JCX increased caspase-3 activation and generation of reactive oxygen species (ROS). N-acetylcysteine treatment blocked JCX-induced ROS generation and the negative effects on pancreatic cancer cell viability. In addition, JCX down-regulated the levels of phospho-focal adhesion kinase (p-FAK) and phospho-extracellular signal-regulated kinase (p-ERK). Together, these results indicate that JCX induces apoptosis in human pancreatic cancer cell lines through ROS production, downregulating FAK/ERK signaling and activating caspase-3. We propose that JCX-derived compounds represent candidates for the development of alternative medicines for the treatment of pancreatic cancer.

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References

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