• Journal of Korean Society of Forest Science
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• v.94 no.2 s.159
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• pp.103-107
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• 2005

Typical phytoplasma whiches' broom symptoms were observed in Ash (Fraxinus rhynchophylla Hence) in Korea. The symptoms of the disease were showing abnormally small leaves, shorted internodes and proliferation of shoots. Examination of fluorescent and electron microscopy of leaf midribs revealed numerous phytoplasma bodies localized in the phloem tube cells. The phytoplasmas were detected in all the symptomatic samples by the amplification with phytoplasma specific primer pair P1/P7 consistently, and the expected size was 1.8 kb. However, the phytoplasma DNA was not detected in healthy seedlings. Based on sequence analysis of amplified region, this phytoplasma has close homologies with eqilodium phyllody, mulberry dwarf, and aster yellow phytoplasmas, 99.95%, 99.79% and 99.78%, respectively, This phylogetic analysis indicates that ash witches' broom phytoplasma should be classified in the aster yellow group 16SrVI and clearly distinct from the ash yellow group 16SrVII.

• Mycobiology
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• v.48 no.6
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• pp.518-521
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• 2020

Macruropyxis fraxini has been recorded on several species of Fraxinus in China, Japan, Russia (Far East), and North Korea since its first recorded observation as a rust fungus on F. rhynchophylla in Jilin, China, in 1899. In the Korean Peninsula, the rust fungus was first recorded on F. rhynchophylla in 1935, based on four specimens collected at Mt. Kumgangsan, Gangwondo Province, in the North Korean territory. We confirmed this rust in the Korean Peninsula after 82 years. The rust fungus was identified based on morphological characteristics and a molecular phylogenetic analysis. This is the first record of M. fraxini in South Korea.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.3
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• pp.277-283
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• 2023

Fraxinus rhynchophylla Hance (F. rhynchophylla) is a traditional medicinal plant that has been widely used in East Asia and has been used for chronic bronchitis, bacterial dysentery and improved eyesight. F. rhynchophylla contains various type of coumarins such as esculin, esculetin, fraxin and fraxetin. Esculetin possesses versatile activities including antioxidant, anti-inflammatory, antimicrobial, anticancer properties and improvement of atopic dermatitis. However, there is no research on the process of increasing active components in F. rhynchophylla. The objectives of the present study were to apply biotransformation technology to F. rhynchophylla for increasing the content of esculetin, and enhancing anti-inflammatory and antioxidant activities. F. rhynchophylla extract (FRE) treated with viscozyme L (FRE-VL) showed 3.1 times higher content of esculetin than FRE, and exhibited effects such as increased anti-inflammatory activity and DPPH radical scavenging activity. Based on the these results, it is concluded that biotransformed FRE-VL could be potentially applicable as a new active ingredient in the cosmetic field.

• Journal of Bio-Environment Control
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• v.16 no.2
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• pp.135-141
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• 2007

Although the result of the tree height growth after water treatment, Fraxinus rhynchophylla has little affection by the artificial water treatment, the growth showed decreased tendency as the soil moisture decreased, and F. mandshurica showed high growth in relatively high soil moist $78{\sim}90%$ treated area. The growth of root collar diameter of F. rhynchophylla and F. mandshurica also showed decreased tendency as soil moisture decreased. The changes of biomass according to dry weight of root, stem, leaves of F. rhynchophylla demonstrating statistical significance as moisture contents of soil is lower showing decreased biomass tendency and in the treatment of $78{\sim}90%$(A) moisture content showed more than double the higher biomass compare to the treated area of $18{\sim}30%$(D) moisture contents. Also F. mandshurica showed statistical significance in A and D treatment demonstrating differences among each treatment. This can be purported to have physiological effects like weakening of seedling and softening of tissues including leaves as soil moisture decreased. Ultimately it is regarded to the main reason of unsatisfactory growth for F. rhynchophylla and F. mandshurica that are weak to drought resistance. SLA, which is one of the special traits of leaf area of F. rhynchophylla, didn't show statistical significance between moisture process, it demonstrated decreased tendency as the moisture content interval is minimal. LAR and LWR showed increased tendency while moisture content didn't show statistical significance between treatments as they are minimal.

• Korean Journal of Pharmacognosy
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• v.38 no.3 s.150
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• pp.287-290
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• 2007

Phytochemical investigation of the MeOH extract of the dried stem barks of Fraxinus rhynchophylla Hance (Oleaceae), as guided by cytoprotective activity against tert-butyl hydroperoxide (t-BHP)-induced cell injury in mouse hippocampal HT22 cells, furnished two coumarins, esculetin (1) and fraxetin (2). Compounds 1 and 2 had the significant cytoprotective effects on t-BHP-induced cellular oxidative injury in HT22 cells. Furthermore, compounds 1 and 2 showed potent DPPH radical scavenging effect, exhibiting $IC_{50}$ values of 14.68 and 9.64 ${\mu}M$, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.1
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• pp.89-94
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• 2014

Stem bark extracts of Fraxinus rhynchophylla Hance were found to contain two major bioactive components, esculetin and esculin. Esculetin substantially inhibited melanogenesis in B16F10 melanoma cells, with an $IC_{50}$ value of $2.8{\mu}M$, and reduced melanin synthesis in Melan-A cells. Moreover, esculetin suppressed melanin biosynthesis by inhibiting mushroom tyrosinase activity, with an $IC_{50}$ value of $40{\mu}M$. Taken together, these results suggest that esculetin could serve as an effective skin-lightening agent that inhibits melanin production by regulating the activity of melanogenic enzymes.

• Food Science and Biotechnology
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• v.16 no.6
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• pp.988-993
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• 2007

The protective effect of the water extract of Fraxinus rhynchophylla leaves (FLE) was determined using an animal model of acetaminophen (AAP)-induced nephrotoxicity. The BALB/c male mice used in this study were divided into 3 groups; the normal, AAP-administered, and FLE-pretreated AAP groups. A single dose of AAP induced necrosis of renal tubules and congestion along with edema to a remarkable degree as observed by hematoxylin and eosin stain, and also increased the numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive renal tubular epithelial cells. Blood urea nitrogen and plasma creatinine levels were determined to be significantly higher in the AAP group than in the normal group. However, FLE pretreatment resulted in an attenuation of renal tubule necrosis. Regeneration and dilatation of renal tubules were noted, and the numbers of TUNEL-positive cells were reduced in the FLE-pretreated groups. In an effort to detect the bioactive compounds exerting protective effects in FLE, the analysis of phenolic compounds via gas chromatography/mass spectrometry (GC/MS) were performed, and identified esculetin and esculin. The present study indicates that these compounds may exert a protective effect against AAP-induced nephrotoxicity.

• Korean Journal of Environment and Ecology
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• v.18 no.2
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• pp.150-157
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• 2004

To investigate the vegetation structure of mountain ridge from Suryeong to Sosagogae, 10 plots(500$m^2$) set up with random sampling method were surveyed. Three groups, Quercus dentata-Fraxinus rhynchophylla community, Quercus mongolica-Fraxinus rhynchophylla community, Quercus mongolica community, were classified by cluster analysis. Quercus mongolica was found as a major woody plant species in the ridge area from Suryeong to Sosagogae. Quercus dentata and Fraxinus rhynchophylla were occupied partly in lower elevation. Species diversity(H') of investigated groups was ranged from 1.7295∼2.6525 and it was similar to that of the ridge area of the national parks in Baekdudaegan. Rare and endangered species, Rhododendron tschonoskii recorded from the list of the Forest Administration distributed between the rocks on the top of the Sambongsan, the long-term habitat monitoring might be required.

• Journal of the Korean Wood Science and Technology
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• v.26 no.4
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• pp.56-60
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• 1998

The bark of Fraxinus rhynchophylla, one of ash trees grown in domestic, was collected, extracted with acetone-$H_2O$(7:3, v/v) and freeze dried to give 50g of ethylacetate soluble powder. A portion of the freeze dried powder was chromatographed on a Sephadex LH-20 and a TSK 40F column using a series of aqueous methanol and ethanol-hexane mixture as eluents. The extractives contained a large amount of coumarin derivatives in addition to a small amount of ester compounds such as ligstroside and oleuropein which have not been isolated from this tree species yet. Some spectrometric analyses such as NMR and FAB-MS including TLC were performed to identify the structures of the isolated ester compounds.

• Korean Journal of Medicinal Crop Science
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• v.21 no.1
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• pp.7-19
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• 2013

This study was carried out to select new pelleting binder and material for seeds from Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. The optimum treatments of the various concentrations and species of priming agents to improve seed germination of both woody medicinal plants were also estimated. Germinability was increased when the seeds of Fraxinus rhynchophylla Hance was soaked in -1.0 MPa of PEG6000 solution at $15^{\circ}C$ for 4 days significantly, the optimum treatment for improving germination of Alnus sibirica Fisch. ex Turcz was observed when the tested seeds was soaked in 100 mM of KCl at $15^{\circ}C$ for 4 days. The influence of physical and chemical properties of pelleting solid materials, the mixture of gypsum, diatomaceous earth, dalma ceramic and vermicuolite (6:1:1:1 ratio) were found as the best pelleting materials for Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. seeds. To satisfy the requirements of absorption and compatibility for multi-layer seed pelleting, SGPA (Starch-grafted cross-linked polyacrylates) hydrogel was prepared using starch, acrylonitrile, ceric ammonium nitrate, nitric acid, methyl alcohol and potassium hydroxide. The resulting SGPA hydrogel showed high water absorption but not plant compatibility. It suggested that seed pelleting using pelleting materials and SGPA hydrogel (multi-layer coating) after priming agent treatment is to increase germinability and seedling growth and it can reduced irrigation labours and can also save seed.